Unit 6 - Gene Expression and Regulation - topic 1 Flashcards
in the 1950’s Rosalind Franklin performed _______ of DNA
X-Ray Crystallography
Edwin Chargaff found that
amount of adenine = amt of thymine
amount of cytosine = amt of guanine
purines
double ring structure
A, G
pyrimidines
single ring structure
C, U, T
hydrogen bonds
what base pairs are held together by
adenine and thymine have
two hydrogen bonds
cytosine and guanine have
three hydrogen bonds
Rosalind Franklin found
helix shape
Chargaff found
base pairing
Watson and Crick combined the findings of Franklin and Chargaff to create the first
3D double helix model of DNA
DNA is a
double stranded helix
backbone
sugar-phosphate
DNA strands are
antiparallel
one strand runs 5’ to 3’, other runs in opposite (3’ to 5’)
5’ end
free phosphate group
3’ end
free hydroxyl group
DNA is a primary source of
heritable information
genetic information is
stored in and passes from one generation to the next through DNA
exception to key function of DNA
RNA is the primary source of heritable information in some viruses
eukaryotic cells
DNA found in nucleus
linear chromosomes
prokaryotic cells
DNA is in nucleoid region
chromosomes are circular
prokaryotes (and some eukaryotes) also contain
plasmids
plasmids replicate
independently from the chromosomal DNA
plasmids
small, circular DNA molecules that are separate from the chromosomes
plasmids contain genes that may be
useful to the prokaryote when it is in a particular environment, but may not be required for survival
plasmids can be
manipulated in laboratories
plasmids can be removed from
bacteria, then a gene of interest can be inserted into the plasmid to form recombinant plasmid DNA
when the recombinant plasmid is inserted back into the bacteria
the gene will be expressed
bacteria can
exchange genes found on plasmids with neighboring bacteria
once DNA is exchanged
the bacteria can express the genes acquired. helps with survival of prokaryotes
RNA
ribonucleic acid
contains ribose
single stranded
A=U and C=G
DNA
deoxyribonucleic acid
doesn’t contain ribose
double stranded
A=T and C=G