Unit 6 - Gene Expression and Regulation - topic 1 Flashcards

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1
Q

in the 1950’s Rosalind Franklin performed _______ of DNA

A

X-Ray Crystallography

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2
Q

Edwin Chargaff found that

A

amount of adenine = amt of thymine
amount of cytosine = amt of guanine

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3
Q

purines

A

double ring structure
A, G

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4
Q

pyrimidines

A

single ring structure
C, U, T

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5
Q

hydrogen bonds

A

what base pairs are held together by

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6
Q

adenine and thymine have

A

two hydrogen bonds

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7
Q

cytosine and guanine have

A

three hydrogen bonds

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8
Q

Rosalind Franklin found

A

helix shape

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9
Q

Chargaff found

A

base pairing

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10
Q

Watson and Crick combined the findings of Franklin and Chargaff to create the first

A

3D double helix model of DNA

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11
Q

DNA is a

A

double stranded helix

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12
Q

backbone

A

sugar-phosphate

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13
Q

DNA strands are

A

antiparallel
one strand runs 5’ to 3’, other runs in opposite (3’ to 5’)

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14
Q

5’ end

A

free phosphate group

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15
Q

3’ end

A

free hydroxyl group

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16
Q

DNA is a primary source of

A

heritable information

17
Q

genetic information is

A

stored in and passes from one generation to the next through DNA

18
Q

exception to key function of DNA

A

RNA is the primary source of heritable information in some viruses

19
Q

eukaryotic cells

A

DNA found in nucleus
linear chromosomes

20
Q

prokaryotic cells

A

DNA is in nucleoid region
chromosomes are circular

21
Q

prokaryotes (and some eukaryotes) also contain

A

plasmids

22
Q

plasmids replicate

A

independently from the chromosomal DNA

23
Q

plasmids

A

small, circular DNA molecules that are separate from the chromosomes

24
Q

plasmids contain genes that may be

A

useful to the prokaryote when it is in a particular environment, but may not be required for survival

25
Q

plasmids can be

A

manipulated in laboratories

26
Q

plasmids can be removed from

A

bacteria, then a gene of interest can be inserted into the plasmid to form recombinant plasmid DNA

27
Q

when the recombinant plasmid is inserted back into the bacteria

A

the gene will be expressed

28
Q

bacteria can

A

exchange genes found on plasmids with neighboring bacteria

29
Q

once DNA is exchanged

A

the bacteria can express the genes acquired. helps with survival of prokaryotes

30
Q

RNA

A

ribonucleic acid
contains ribose
single stranded
A=U and C=G

31
Q

DNA

A

deoxyribonucleic acid
doesn’t contain ribose
double stranded
A=T and C=G