Unit 6 Flashcards
Explain the reason for the variation in cell shape
Function form are interrelated.
Describe the two ways of studying cells
Microscopy:
-light
Brightfield, fluorescent, phase contrast
-electron
S.E.M. And T.E.M
Biochemistry:
Cell fractionation
Can separate organelles or protein
Complexes. Cintrifical force diagram on slides
Describe prokaryotes cells
Prokaryotes – end domain bacteria, and domain archaea
Average diameter of two micro meters
Nearly all have DNA in a nucleode region(no nucleus), circular chromosomes, phospholipid based plasma membrane, 70s ribosomes, cytoplasm
Some have: a proteoglycan cell wall, glycocalyx, fimbriae, flagella
Describe eukaryotic cells
Eukaryotes – domain eukaryote; kingdoms, and Animila, Plantia, Protista , fungi
10 to 100 µm in diameter varying shapes, and sizes
Nearly all have: plasma membrane, DNA in the nucleus, linear chromosomes, 80s ribosomes, internal membranes and membrane bound organelles, mitochondria
Some have chloroplasts (plants and some protists), cell walls(Cellulose in plants chitin in fungi), vacuoles for storage, Celia, flagella
Venn diagram, comparing contrast, prokaryote and eukaryote cells
Be able to identify basic regions/structure of a eukaryote cell
Plasma membrane nucleus, cytoplasm
Describe the structure of the plasma membrane
The plasma membrane is described by the fluid Mosé model
The plasma membrane is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell
What is the role of phospholipids proteins and carbohydrates in the plasma membrane?
Describe what is meant by the term selectively permeable
The lipid membrane provides a barrier to large molecules and charged molecules/ions
The lipid membrane does not provide a barrier to nonpolar molecules, such as lipids or steroids or molecules like H2 and CO2
Be able to describe the structure and function of the nucleus
The functions of the nucleus are
DNA storage
Replication
Transcription
RNA production
mRNA, rRNA, tRNA production
What are the main organelles in the cytoplasm and their functions?
Mitochondria: the powerhouse of the cell, responsible for producing ATP through cellular respiration
Endoplasmic reticulum: network of membranes involved in protein and lipid synthesis. It has two types: rough and plasmic reticulum (ribosomes) and smooth endoplasmic reticulum (no ribosomes)
Golgi Apparatus: processes, swords, and ships proteins and lipids for secretion or use within the cell
Lysosomes: contain enzymes that digest and recycle cellular waste and foreign materials
Ribosomes: structures responsible for protein synthesis by translating mRNA
Proxy: breakdown fatty acids, and detoxify harmful substances like hydrogen peroxide
Centrioles: Involved in organizing microtubials and care important during cell division (mitosis and meiosis)
Ribosome structure
Structure
Two subunits(one larger one smaller)
Made of rRNA and proteins
Subunits assemble after mRNA binds
Bacteria have 70 S ribosomes, smaller than eukaryote 80 s ribosomes
Ribosome function
Located in the cytoplasm
Translates mRNA information to build proteins
Sometimes the mRNA sequences make the ribosome dock to the rough endoplasmic reticulum and make proteins in the end membrane system
Describe the structure of the endomembrane system
Parts:
Nuclear envelope
Smooth and rough endoplasmic reticulum
Golgi apparatus
Lysosomes
Vacuoles
Describe the function of the endomembrane system
Functions:
Many functions
But primary functions is to make, modify, and transport port proteins Destined for the Plasma membrane, the endomembrane system, or the extra cellular space
Describe the structure of the smooth endoplasmic reticulum
Extensive membrane bound compartment
Continues with nuclear envelope
Lacks bound ribosomes
Describe the function of the smooth endoplasmic reticulum
Steroid production
Detoxification
Calcium storage
Carbohydrate metabolism
Describe the structure of the rough endoplasmic reticulum
Many ribosomes attached to the outer surface
Cisternae- flattened, stacked membrane sacks, forming the main structure of the RER
Continues the nuclear envelope
Describe the structure of the Golgi apparatus
Stacks of flattened cisternae and associated vesicles. Has a cis and trans. Proteins and lipids from the endoplasmic reticulum enter at the cis face exit at the trans face
Describe the functions of the rough endoplasmic reticulum
Ribosome docking
Folding the protein (chaperones)
Initial glycosylation
Transport to Golgi
RER also makes Phospholipids
Describe the function of the Golgi apparatus
Post transitional modifications (primarily glycosylation)
Packaging and sending proteins
Retrograde transport of the Golgi
Describe the function of lysosomes and plasma memory
Low pH digestive structure
Breaks down misfed endomembrane proteins
Breakdown and engulfed substances/bacteria
PM functions are related to the endomembrane
Site of exocytosis
Vesicles fused to plasma membrane to deliver TM proteins or ECM proteins
Site of endocytosis, phagocytosis
Glycosylated proteins must work on the surface for cell communication and other functions
Acton protein is used in the cytoplasm. Describe how the acting gene produces the act and protein. No locations of events.
The epidermal growth factor receptor(EGFR) is a trans membrane protein embedded in the plasma membrane. Describe how the EFR gene produces the protein. No locations.