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NAS2 Challenge Exam Prep > Unit 4: Cells > Flashcards

Unit 4: Cells Flashcards

1
Q

Organelle function: Cell membrane/plasma membrane

A

Surrounds the cell; Keeps stuff in and out

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2
Q

Organelle function: Cytoplasm

A

Internal cell fluid which holds all other structures inside the cell

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3
Q

What are cell membranes composed of?

A

Phospholipids

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4
Q

What are phospholipids comprised of?

A

Hydrophilic head: Glycerol + Phosphate
Hydrophobic tails: 2 fatty acids (one saturated, one unsaturated)

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5
Q

What’s the polarity of the phosphate head?

A

Polar covalent bonds.
Hydrophilic

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6
Q

What’s the polarity of the fatty acid tails?

A

Nonpolar
Hydrophobic

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7
Q

What’s the organization of the phospholipid bilayer?

A

Head – Tails – Tails – Head

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8
Q

Do phospholipids interact with one another in the bilayer?

A

There are no chemical bonds between individual phospholipids.
A very flexible and fluid mosaic
Allows for movement of membrane and proteins embedded in the membrane

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9
Q

In what way does the cell membrane allow transport?

A

Selective permeability
The hydrophobic interior (brought to us by the hydrophobic fatty tails) prevents the transport of most molecules
It allows lipids and other small nonpolar molecules (such as O2) and very small polar molecules (such as H2O)

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10
Q

What are the two structure types of proteins that are contained in the cell membrane?

A

Integral and Peripheral
Integral proteins stick out on either end (span the entire membrane)
Peripheral proteins just stick out on one end

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11
Q

What’s the function of this protein: Receptors

A

Outside substances bind to them

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12
Q

What’s the function of this protein: Enzymes

A

Speed up the chemical reactions on the inside of outside of the cell

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13
Q

What’s the function of this protein: Aquaporins

A

Move water inside or outside the cell

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14
Q

What’s the function of this protein: Channels

A

Move other substances inside or outside the cell

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15
Q

What is cytoplasm comprised of?

A

Cytosol: water, ions, enzymes
Organelles

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16
Q

Organelle function: Nucleus

A

Holds the DNA
Houses the nucleolus
Bound by a double membrane nuclear envelope
Has nuclear pores to allow transport of particular molecules in/out

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17
Q

What is the nucleolus?

A

Where ribosomes are made and RNA is transcribed

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18
Q

What is chromatin?

A

A complex of DNA and proteins that forms the chromosomes within the nucleus
Exists in two forms: Euchromatin (less condensed) and Heterochromatin (condensed)

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19
Q

What are ribosomes?

A

Made of RNA and protein- rRNA
Make proteins using the info from RNA

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20
Q

Organelle function: Mitochondria

A

Contains its own DNA and ribosomes
Site of cellular respiration

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21
Q

What is cellular respiration?

A

The process of breaking glucose down to get energy

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22
Q

Organelle function: Peroxisomes

A

Breaks down toxic molecules

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23
Q

Organelle function: Vesicles

A

How proteins and other chemicals move around a cell
A membrane bubble
Travels using the endomembrane system:
- Smooth/rough ER
-Golgi apparatus

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24
Q

Organelle function: Smooth Endoplasmic reticulum (ER)

A

Houses enzymes to break down drugs and poisons; synthesizes lipids
Further away from the “maze”

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25
Q

Organelle function: Rough Endoplasmic reticulum (ER)

A

Helps with protein synthesis, folding, and sorting
Has ribosomes on its surface (making it appear “rough”)

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26
Q

Organelle function: Golgi apparatus

A

Assists with protein folding and modification
Repackages proteins into vesicles and sends them towards the cell membrane

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27
Q

What are lysosomes?

A

The digestive system of the cell, breaks down molecules for parts

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28
Q

Cytoskeleton composition from smallest to largest:

A

Microfilaments –> Intermediate filaments –> microtubules
These are all involved in cell structure and molecule movement within the cell

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29
Q

Microtubules?

A

Used as a scaffolding during cell growth/division

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30
Q

Microfilaments?

A

Strengthen and change the shape of a cell

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31
Q

Intermediate filaments?

A

Main framework of cells

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32
Q

What organelles organize the microtubules?

A

Centrosomes made up of 2 centrioles

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33
Q

Which cellular extensions are on the inside versus outside of the cell?
Microvilli, cilia, flagella

A

Microvilli are finger-like projections on the inside of the cell membrane to increase surface area
Cilia and flagella are on the outside of the cell

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34
Q

What are cilia?

A

Short and fringe-like
Has coordinated movements like a wave to move particles in a certain direction

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35
Q

What’s an example of cilia?

A

Cells lining the trachea

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36
Q

What are flagella?

A

Longer structures that exist by themselves or in pairs
Function like a propeller

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37
Q

What’s an example of a flagella?

A

Sperm cells

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38
Q

What is the break down of a 1% NaCl solution? (What is its concentration?)

A

1 molecule of NaCl for every 99 water molecules

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39
Q

When there’s a concentration gradient…

A

There’s a different concentration of molecules on one side of a membrane

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40
Q

Define: Hypertonic, Isotonic, Hypotonic

A

Hypertonic: a solution with higher solute concentration
Isotonic: a solution with equal solute concentration
Hypotonic: a solution with less solute concentration

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41
Q

When do molecules NOT move from an area of high concentration to low concentration?

A

If a barrier is present restricting movement

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42
Q

Diffusion is…

A

Molecules moving down a gradient from high concentration to low concentration
More molecules will get bumped out than those that get bumped in

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43
Q

Solution A is 30% sugar- is the solution hypothonic/hyper/iso/none?

A

None! We have to compare it to another solution

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44
Q

Solution A is 30% sugar, solution B is 10% sugar…

A

Solution A is hypertonic to solution B

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45
Q

Inside the cell is 98% water, outside the cell is 70% water

A

The cell is hypertonic to surroundings

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46
Q

When molecules don’t require energy for assistance when passing through a membrane….

A

Passive transport

47
Q

When molecules require some energy for assistance when moving through a membrane…

A

Active transport

48
Q

Passive transport is always from ___ to ___

A

High to low concentration

49
Q

What is simple diffusion?

A

Movement of small nonpolar molecules freely through the membrane (passively transported)
Ex. O2, CO2

50
Q

What is facilitated diffusion?

A

Movement of polar molecules with the assistance of a protein
Ex. glucose
It’s still a passive transport as the solute is moving down the concentration gradient

51
Q

What is osmosis?

A

Movement of water (even though its polar) across a membrane (passive transport)

52
Q

Active transport is always from ___ to ___

A

Low to high concentration

53
Q

What is primary active transport?

A

A form of active transport where ATP molecules are required to move from a low to high gradient (“against the gradient” NOT with)
Uses chemical energy

54
Q

What is an example of primary active transport?

A

The sodium-potassium pump, where sodium ions are moved to the outside of the cell and potassium ions are moved to the inside of the cell

55
Q

What is secondary active transport?

A

A form of active transport where one molecule is required to move down its gradient to allow/power a different molecule to move up the gradient

56
Q

Example of secondary active transport?

A

The sodium-glucose pump, where cotransport by active sodium enzymes facilitate glucose moving upstream (2 sodiums for 1 glucose)

57
Q

What is vesicular transport?

A

Active transport of large molecules too big to pass through a cell membrane or transport protein. It requires the use of vesicles

58
Q

What is exocytosis?

A

Movement of molecules out of a cell (export)

59
Q

What is endocytosis? What are its subcategories?

A

Movement of molecules into a cell (import).
Phagocytosis (large molecules)
Pinocytosis (large volumes of extracellular fluid)

60
Q

What is transcytosis?

A

A combination of endocytosis and exocytosis to move a molecule through a cell

61
Q

What are the 5 nitrogenous bases found in nucleotides?

A

Adenine, Thymine
Cytosine, Guanine, Uracil

62
Q

Which nitrogenous bases are pyrimidines?
Which are purines?

A

Pyrimidines: C, T, and U
Purines: A, G

63
Q

The complementary nitrogenous bases form what kind of bonds?

A

Hydrogen bonds
A-T
G-C

64
Q

DNA copying is called…

A

Replication

65
Q

Before DNA replication, chromosomes are made of one piece of double stranded SNA. After replication, replicated chromosomes are made of two pieces of identical double-stranded DNA called…

A

Sister chromatids

66
Q

Both replicated and unreplicated chromosomes count as ___ chromosome

A

One

67
Q

In RNA, what nitrogenous base is changed to uracil?

A

Thymine is changed to Uracil

68
Q

How do the functions of DNA and RNA differ?

A

DNA stores information
RNA uses information (to make proteins, catalyze reactions, etc.)

69
Q

What are the three major types of RNA?

A

Messenger (mRNA): Encodes proteins
Transfer (tRNA): Assists in protein synthesis (translation)
Ribosomal (rRNA): Builds ribosomes

70
Q

Fill in the blanks:
DNA –> RNA –> Protein
_____ ______ ______

A

Replication, Transcription, Translation

71
Q

DNA Replication vs Transcription:
Final molecules?

A

DNA Replication: 2 identical DNA molecules
Transcription: 1 RNA molecule that is not identical to the original DNA molecule

72
Q

DNA Replication vs Transcription:
Nucleotides used?

A

DNA Replication: A-T, G-C
Transcription: A-U, G-C

73
Q

DNA Replication vs Transcription:
Number of DNA strands copied?

A

DNA Replication: 2
Transcription: 1

74
Q

DNA Replication vs Transcription:
Enzyme used?

A

DNA Replication: DNA polymerase
Transcription: RNA polymerase

75
Q

DNA Replication vs Transcription:
Region copied?

A

DNA Replication: the whole DNA molecule
Transcription: a small segment; one gene

76
Q

Where does transcription take place?

A

DNA –> RNA takes place in the nucleus

77
Q

What are the steps in transcription?

A
  1. Initiation
  2. Elongation
  3. Termination
78
Q

What is the initiation stage?

A

RNA polymerase binds to the promoter (the DNA sequence indicating the start of a gene)

79
Q

What is the elongation stage?

A

The DNA double helix opens at a promoter
RNA polymerase builds an RNA strand complementary to one strand of DNA (pairing A with U not T)

80
Q

What is translation?

A

RNA is used to make a protein via the ribosomes in the cytoplasm/on the rough ER
The ribosomes read the mRNA sequence, and corresponding amino acids are bonded together to make the protein

80
Q

What is the termination stage?

A

RNA is released from DNA at the terminator sequence, the DNA goes back together
The RNA is now ready to make a protein

81
Q

How many kinds of amino acids are there?

A

20

82
Q

How many nucleotides make one amino acid? What are they called?

A

3; a codon

83
Q

Which RNA reads mRNA and brings the correct amino acid in translation?

A

Transfer RNA (tRNA)
It has 2 attachment sites: one for the amino acid, and one where it attaches to the mRNA inside the ribosome (Anticodon)

84
Q

Where do the amino acids come from to make proteins?

A

From the cytoplasm, either from nutrients or they are made in the cell

85
Q

What is the translation start codon?

A

Met

86
Q

What kind of bond forms between the starting codon and the following amino acid?

A

Peptide bond

87
Q

What happens when a ribosome reaches a stop codon?

A

mRNA and amino acid chain are released
The ribosomal subunits seperate

88
Q

Can mRNA be translated by multiple ribosomes simultaneously?

A

Yes! the mRNA is broken down more quickly afterwards
If more protein is needed, the DNA sequence will need to be transcribed again

89
Q

Transcribe the DNA sequence to RNA, then the RNA to an amino acid sequence:

3’ - ATA CAC GTC TAG CAG - 5’

A

5’ - UAU GUG CAG AUC GUC- 3’
Tyr Val Gln Ile Val
RNA builds from the 5’ to 3’ direction, adding each new nucleotide to the 3’ end of the strand

90
Q

What is the purpose of mitosis?

A

Cell division for growth and development

91
Q

What is the purpose of meiosis?

A

Cell division for sex cell/gamete production and ultimately making new organisms

92
Q

What is interphase?

A

Where a typical cell spends most of its life. It isn’t replicating, it’s just obtaining nutrients, metabolizing, growing, and preparing itself for mitosis

93
Q

What happens in G1?

A

Normal cell business and protein synthesis

94
Q

What happens in the S phase?

A

DNA replication, DNA repairs, protein synthesis, and duplication of the centrosome

95
Q

What is the centrosome?

A

Organelles that serve as the main microtubule organizing structure; Regulator of cell division

96
Q

What happens in G2?

A

Preparation for mitosis
Protein synthesis

97
Q

What are homologous chromosomes?

A

Homologous pairs have the same length, shape, and genes (though they CAN have different alleles!)
These can be replicated or unreplicated

98
Q

Before DNA replication, there are ___ chromosomes with __ chromatid per chromosome

A

Before DNA replication, there are unreplicated chromosomes with 1 chromatid per chromosome

99
Q

After DNA replication, there are ___ chromosomes with __ chromatids per chromosome

A

After DNA replication, there are replicated chromosomes with 2 chromatids per chromosome
The centromere is where these sister chromatids attach

100
Q

When do the sister chromatids split apart?

A

During mitosis, one unreplicated chromosome goes to each new cell

101
Q

What is the first phase in mitosis?

A

Prophase
This is when DNA condenses and forms visible chromosomes
The centrosomes move to opposite poles where the microtubules organize and forms the mitotic spindle

102
Q

When does the nuclear envelope break down and spindle fibers attach to centromeres?

A

Prophase

103
Q

What happens during metaphase?

A

Chromosomes line up in the center of the cell at the metaphase plate

104
Q

In what phase do chromatids separate from one another and go to opposite poles, and what are these now called?

A

Anaphase
The separated chromatids are now unreplicated chromosomes

105
Q

What happens during telophase?

A

DNA decondenses (loosens up), the nuclear envelope reforms, spindle fibers break down, and cytokinesis occurs

106
Q

Is cytokinesis a phase?

A

Nope! It is part of telophase.
This describes the physical splitting of the cell where organelles and cytoplasm are split evenly.

107
Q

What happens during cytokinesis for specifically animal cells?

A

A cleavage furrow is formed, where the cell divides from the outside in

108
Q

Which organelle breaks down toxins?

A

Peroxisomes
Lysosomes are for breaking down useful items for recycling (NOT harmful things)

109
Q

What’s the difference between primary active transport and facilitated diffusion?

A

Primary active transport is moving a substance from an area of lower to higher concentration, using ATP

Facilitated diffusion is moving a substance from an area of higher to lower concentration through a protein

110
Q

Given the following sequence of DNA, select the amino acid sequence it encodes:
AGCTACAGCTAGCGAATTGTA

A

Met-Ser-Ile-Ala

111
Q
A
112
Q

What happens first in transcription?
Two DNA strands separate
RNA polymerase attaches to the promoter sequence

A

DNA strands separate before the RNA polymerase attaches to the promoter sequence

NAS2 Challenge Exam Prep (5 decks)

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