Unit 4 Flashcards
curved and comma-shaped, gram-negative rods
vibrio
(true or false)
vibrios are gram-positive rods
false ( gram-negative)
in what family does vibrio belongs to
vibrionaceae
vibrio are suscpetible to what acid?
gastric acid
vibrios that are not halophile
metschnikovii and mimicus
habitat of vibrio
fresh water or salt water
lab diagnosis for vibrio
catalase and oxidase positive
common test for lab diagnosis for vibrio
positive string test
responsible for all pandemics and most epidemics of cholera
O1 serogroups
based on somatic O antigen
serogroups
identified in 1992 and causes cholera outbreaks in India and Bangladesh
O139 serogroup
other name of O139 serogroup
Bengal strain
pathogenesis of cholera
fecal-oral route by ingestion
infective dose of cholera
being acid-labile
hydrolyses protective mucus layer
mucinase
adhesion to intestinal epithelium
toxin coregulated pilus
what fragment binds to the GM
fragment b
what fragment causes ADP ribosylation
fragment a
increases intestinal fluid secretion
accessory cholera toxin
increases intestinal permeability
zonula occludens toxin
modifies cell surface to increase GM
neuraminidase
required for iron acquisition
siderophore
adhesin factor
chemotaxis protein
incubation period of cholera
24-48 hrs
the stool is typically non-bilious
rice water stool
carriers of cholera
healthy people who shed the bacilli in feces
lab diagnosis for cholera
freshly collected watery stool before antibiotics
transport media of cholera
venkatraman-ramakrishnan medium
alkaline salt transport medium
cary-blair medium
autoclaved seawater
what kind of microscopy is used in cholera
direct microscopy
what can be observed in a gram staining of a cholera
fish in stream appearance with darting motility
