Unit 3: Nutritional Requirements

Question 1

why is water important

Answer 1

nutrients must be H2O soluble to be removed from the environment and utilized by bacteria

Question 2

importance of carbon

Answer 2

50% of bacterial cell

most disease producing bacteria require ORGANIC sources e.g. peptones and CHO

Question 3

importance of nitrogen

Answer 3

14% of bacterial cell
for proteins, nucleic acids, coenzymes, cell walls
inorganic sources found in tissues in reduced state and in media e.g. NH4 salts
organic sources required by pathogens peptide digests

Question 4

importance of phosphate + sulphur + trace metals

Answer 4

required for ATP, ADP, coenzymes, proteins, aa, cystine, spore formation, perspiratory enzymes like cytochromes

Question 5

importance of growth factor

Answer 5

org may not be able to synthesize some organic nutrients required for growth. blood in BAP supplies most of organic nutrients

Question 6

describe nutritional requirement of Neisseria gonorrhoeae

Answer 6

requires CO2 and a Choc plate or basal medium that has added casein and animal tissue

Question 7

describe nutritional requirement of Homophiles influenzae

Answer 7

requires CO2, heme portion of hemoglobin and NAD in Choc plate

Question 8

describe nutritional requirement of Mycoplasma

Answer 8

requires many nutrients to grow fatty acids and cholesterol

Question 9

describe nutritional requirement of Bordetella

Answer 9

very susceptible to toxic materials including inhibitory peptones; charcoal (Regan-Lowe agar) and potato starch (BG agar) used as neutralizing agents

Question 10

give examples of growth factors

Answer 10

meat, yeast extract, animal tissue like brain, bodily fluids like serum

Question 11

what is semi fastidious

Answer 11

require blood to grow well often benefits fro increase CO2

Question 12

what is non fastidious

Answer 12

grows on nutrient media in absence of blood in ambient air