Unit 3: Isolation and Quantitation of Bacteria

Question 1

objective of semi quantitative streaking patten

Answer 1

dilute number of org present while isolating every diff org in specimen

Question 2

what is quantitative streaking patten

Answer 2

measured amount of inoculum (e.g. a calibrated loop deliver 0.001 mL urine) spread over plate

Question 3

why do we inoculate from least to most selective

Answer 3

avoid the inhibition of org by selective agent

Question 4

amounts reported for semi quantitative streaking patten

Answer 4

+4,3,2 or 1. heavy, moderate, light or scant respectively

Question 5

amounts reported for quantitative streaking patten

Answer 5

__ x 10^6 CFU/L. convention converts # of org in 1/1000 mL to # that would be present in a litre