Unit 3- Molecular Genetics Flashcards
Okazaki fragments
The new piece of DNA on the lagging strands
Chargaff rule
If you know the nucleotide sequence of one strand you can uncover the sequence of the other strands
Chromosomes
A threadlike strand of DNA in the cell nucleus that carries the genes in a linear order
Locus
Specific location on a gene
Gene
A distinct sequence of nucleotides forming part of a chromosome
DNA molecule
Pentose sugar
Phosphate group
Nitrogenous base
How is the nitrogenous base attached to the carbon ring?
Glycosyl bond
How is the phosphate group attached to the 5’ carbon?
Ester bond
Complementary base pairing
Purines are always bonded to a pyrimidine
Meselson and Stahl model 1
The original parent DNA is conserved and not altered in any way. The daughter DNA molecule will be made entirely from free floating nucleotides in the nucleus
Meselson and Stahl’s model 2
Half the parent DNA will be used in the construction of the new DNA molecule. This half is conserved (semi conserved). Thus it is half parent cell and half free floating nucleotide
M and S experiment
Step1 grow E. coli bacteria in the presence of N15 only (parent)
Step2 take those bacteria and allow them to reproduce in the presence of N14 (gen 1)
Step3 take gen 1 bacteria and again allow them to reproduce in N14 (gen 2)
M and S conclusion
The DNA that contains N15 are heavier