Unit 3: Cellular Energetics Flashcards
Metabolism
ALL CHEMICAL RXNS: Totality of an organism’s chemical rxns
Manage materials & Energy resources of a cell
EX: apoptosis, growing hair, being awake/alive, digestion
Metabolism RXNs
A (start molecule) -> RXN 1 (enzyme A) -> RXN 2 (enzyme B) -> RXN 3 (enzyme C) -> D (product)
Each RXN has own enzyme w/ diff shapes for diff functions
Enzymes dont get used up in RXN js help facilitate them
Catabolic Pathway
Release Energy by BREAKING down
complex molecules into simpler compounds (gain energy)
Ex: digestive enzymes break down food -> release Energy, Hydrolysis, weightloss pills
Anabolic Pathway
CONSUME Energy to BUILD complex
molecules from simpler ones
Ex: amino acids link to form muscle protein, Dehydration synthesis, Steroids
Energy
Capacity to DO WORK
Kinetic Energy
Movement, Energy associated w/ Motion
Ex: HEAT (thermal energy) is KE associated w/ random movement of atoms or molecules (how fast molecules are moving)
Potential Energy
Position-based ex: Someone at top of Building, STORED energy as a result of its position/ structure
Ex: Chemical energy is PE available for release
Energy can be…
ONLY converted from 1 form to another (Never created/destroyed)
Ex: chemical -> mechanical -> electrical (forms of PE)
Thermodynamics
Study of Energy Transformations that occur in matter
Closed System
Isolated from its surroundings
(ex: liquid in a thermos)
Open System
Energy & Matter can be TRANSFERRED between system & surrounding
Ex: organisms
1st Law of Thermodynamics (Conservation of E)
Energy of Universe = Constant
E CANT be created or destroyed only transformed/transferred
2nd Law of Thermodynamics
Every E transfer/transformation, increase Entropy (disorder) of universe
During every E transfer/ transformation, some
E is unusable, often LOST as HEAT
Free Energy
Part of system’s E available to perform work
ΔG = change in free energy
Exergonic RXN
Energy is RELEASED
Spontaneous RXN
ΔG < 0
Hydrolysis/Catabolic
Endergonic RXN
Energy is REQUIRED
Absorb Free energy
ΔG > 0
Dehydration Synthesis/Anabolic
Is a living cell at Equilibrium?
NO, constant flow of materials in/out of cell
What 3 types of WORK do cells do?
Mechanical, Transport, Chemical
What is coupling?
Cells MANAGE Energy resources to do WORK by Energy coupling
Using an Exergonic process (up) to drive an Endergonic one (Rollercoaster)
ATP
(Adenosine TriPhosphate)
Cell’s MAIN Energy source in Energy Coupling
Adenine + ribosomes + 3 phosphates (Ex: Nucleic Acids)
How does pH affect enzymes?
Changing the pH outside of this range will slow enzyme activity. Extreme pH values can cause enzymes to denature.
How does temperature affect enzymes?
Raising temperature generally speeds up a reaction, and lowering temperature slows down a reaction
Extreme high temperatures can cause an enzyme to lose its shape (denature) and stop working
How does Hydrolysis affect bonds between phosphate groups of ATP?
When the bonds between the phosphate groups are broken by hydrolysis → Energy is released
*Release of E comes from Chemical Change to State of Lower Free E, NOT in the Phosphate Bonds themselves
How ATP Preforms WORK
Exergonic release of Pi is used to do the Endergonic work of cell
When ATP is hydrolyzed, it becomes ADP (adenosine diphosphate)
Transport Work
ATP phosphorylates Transport Proteins
Mechanical Work
ATP Binds non-covalently to motor proteins & then is HYDROLYZED -> Pi + ADP (walking protein)
Catalyst
Substance that can change Rate of a RXN W/OUT being altered in the process (by lowering activation energy)
ex: Enzyme = biological catalyst
Example of Catalyst
Sucrase BREAKS apart Sucrose enzyme, hydrolysis)
Speeds up Metabolic RXN by lowering activation Energy (needed to start RXN by breaking bonds)
W/O Enzyme = slower & less efficient
Substrate Specificity of Enzymes
The reactant that an Enzyme acts on is called the enzyme’s substrate
The enzyme binds to its substrate, forming an enzyme-substrate complex
Active site of Enzyme
Region on Enzyme where
substrate binds
Cycle of Enzyme
1) Substrates enters Enzyme’s Active site
2) Substrates held in Active Site by weak interactions
3) Substrates -> converted to products
4) Products are released
5) Active site available for new substrates
Induced Fit
Enzyme fits SNUGLY AROUND SUBSTRATE
“CLASPING HANDSHAKE”
Enzyme’s Activity can be Affected by?
Temperature, pH, Chemicals
bc Enzyme = protein & denatures sp ruins shape/function
Ex: stomach = low pH (acidic) so can digest stuff by enzymes
Cofactors
Nonprotein Enzyme helpers such as minerals (Ex: Zn, Fe, Cu) (bc body needs control)
Coenzymes
Organic Cofactors (ex: vitamins)
Enzyme Inhibitors
Competitive & Noncompetitive
Competitive Inhibitor
Binds to the Active Site of an
enzyme, competes w/ substrate (which will eventually win) & Enzyme will work again
Noncompetitive Inhibitor
Binds to Another part of Enzyme -> changes shape -> Active Site is Nonfunctional (SHUTS ENZYME DOWN FOREVER) bc substrate wont ever bump out noncompetitive (changes shape/function) Until enzyme destroyed
Regulation of Enzyme Activity
To regulate metabolic pathways, the cell switches on/off genes that encode specific enzymes
Allosteric Regulation
DIFF SHAPE REGULATOR
Protein’s Function at one site
is affected by binding of a regulatory molecule to a separate site (allosteric site)
Activator
Stabilizes Active Site
Inhibitor
Stabilizes Inactive form
Cooperativity
One substrate triggers shape change in other active sites → Increase catalytic activity
Feedback Inhibition
Series of Enzyme & RXN need to turn off/on certain things for balance
END PRODUCT of a Metabolic pathway shuts down Pathway by Binding to the Allosteric site of an enzyme
Prevent wasting chemical resources, increase efficiency
of cell
EX: make stomach pepsin & acid only when eating to digest food
Another Example of Feedback Inhibition
Threonine turns into isoleucine -> isoleucine shuts off threonine when enough bc wont have any more threonine (no waste) only makes more isoleucine when necessary no overproduction
How does chemicals denature enzymes?
competitive & noncompetitive inhibitors! MAINLY noncompetitive dont compete w/ substrate
In Open Systems, Plant Cells Require E to Preform Work (Chemical, Transport, Mechanical)
1) E flows into ecosystem as sunlight
2) Autotrophs (self, eat) transform it -> chemical E (O2 released as byproduct)
3) Cells use some of Chemical E in organic molecules to make ATP
4) Extra E LEAVES as HEAT
- Complex Organic Molecules
Take Catabolic (break down) pathway to make simpler waste products w/ less E & some E used to do work & dissipated as HEAT
Cellular Respiration (Exergonic Releases E) - EQ:
C6H12O6(glucose) + 6O2(oxygen) → 6H2O(water) + 6CO2 (carbon dioxide)+ ATP (+ heat)
Photosynthesis (Endergonic, Requires/Absorbs E)
6H2O(water) + 6CO2(carbon dioxide) + Light (sun) → C6H12O6(glucose) + 6O2(oxygen)
***** Why is Oxygen important?
TERMINAL ELECTRON ACCEPTOR
Energy Harvest
Energy is released as electrons “fall” from organic molecules to O2
Steps: Food (Glucose) → NADH → ElectronTransportChain → Oxygen
Steps of Energy Harvest
1) Coenzyme NAD+ = electron acceptor
2) NAD+ picks up 2e- and 2H+ → NADH (stores E)
3) NADH carries electrons to the electron transport chain (ETC)
4) ETC: transfers e- to O2 to make H2O ; releases energy (ATP!!!)
NAD+ acts AS?
Electron Shuttle: energy from food, rips hydrogens & electrons from them
Electron Transport Chain (ETC)
Gains Free E from hydrogens (high in lipids = lots of calories)
Cellular Respiration: (Controlled steps) Slowly Releases Energy instead of explosion (uncontrolled) = staying alive