Unit 3: Cells Flashcards

1
Q

Name and describe the 3 main parts of a cell.

A

1) The Plasma membrane: forms the cell’s outer surface. Which then manages the internal environment and acts as a gatekeeper to anything coming into and out of the cell.
2) Cytoplasm: consists of the cellular contents between the plasma membrane and the nucleus.
This is divided into 2 parts: cytosol and organelles.
Cytosol is the liquid portion of the cell.
Organelles which has a character structure and specific functions.
3) Nucleus: Acts as the control center for a cell, due to it containing genes. Which controls cellular structure and most cellular activities

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2
Q

What is cell biology

A

is the study of cellular structure and function

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3
Q

What is the Cytosol and what it’s composition?

A

This is the liquid portion of the cell. (intercellular fluid)
It is made up of water plus dissolved solutes and suspended particles. Site of many chemical reactions.

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4
Q

Describe the structure of the Plasma Membrane.

A

The plasma membrane is a flexible, sturdy barrier that surrounds and contains the cytoplasm of the cell.

The fluid mosaic model describes the structure of the plasma membrane, which is composed of a double layer (bilayer) of phospholipid molecules with many protein molecules dispersed within it.

The surfaces of the membrane are hydrophilic due to the polar phosphate heads. The internal portion of the membrane is hydrophobic due to the nonpolar fatty acid tails.

Integral proteins are firmly inserted into and extend across the lipid bilayer. Most of these proteins are glycoproteins, which serve as channels (pores), transporters (carriers), receptors (recognition sites), or enzymes.

Peripheral proteins lie loosely on the inner and outer surface of the cell membrane, serving as enzymes or cytoskeletal anchors.

Membranes are fluid structures, somewhat like cooking oil, because most of the membrane lipids and many of the membrane proteins easily move within the bilayer.

Cholesterol serves to stabilize the membrane and reduce membrane fluidity.

Plasma membranes are selectively permeable, meaning that some things can pass through and others cannot.

The lipid bilayer portion of the membrane is permeable to small, nonpolar, uncharged molecules, but impermeable to ions and charged or polar molecules. This bilayer also is permeable to water.

Transmembrane proteins that act as channels or transporters increase the permeability of the membrane to molecules that cannot cross the lipid bilayer.

Macromolecules are unable to pass through the plasma membrane except by vesicular transport.

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5
Q

Describe the function of the plasma membrane

A

1) Acts as a barrier separating inside and outside of the cell.
2) Controls the flow of substances into and out of the cell.
3) Helps identify the cell to other cells
4) participates in intercellular signalling.

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6
Q

what is meant by Selective Permeability

A

The membrane has selective permeability meaning it is able to control what comes into and out of the cell. The cell allows water and nonpolar (lipid-soluble) molecules to pass through the membrane however does not allow ions, and large, uncharged polar molecules to pass freely

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7
Q

Describe membrane transport, and compare and contrast the different processes.

A

Intracellular fluid is located inside the cell, and extracellular fluid is located outside the cell.

The types of extracellular fluid include the following:

  • Interstitial fluid
  • Plasma
  • Lymph

Solutes are substances dissolved in a solvent.

A concentration gradient is the difference in the concentrations of a substance between two areas (e.g., the amount of water in your cells compared to the amount of water in the lake in which you are swimming, or the amount of sodium ions in extracellular fluid compared to the amount of sodium ions in intracellular fluid).

Transport processes are classified according to two criteria:

  • Active or passive
  • Vesicular

Living cells use three passive transport processes, two of which are nonmediated (diffusion through the lipid bilayer and diffusion through a channel) and one that is mediated (facilitated diffusion).

Vesicular transport involves the formation of membrane-surrounded vesicles to move materials into or out of the cell by endocytosis or exocytosis. See also Objective 5 in this module.

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8
Q

What is simple diffusion and what substances are able to move in and out of the cell?

A

Molecules move down the concentration gradient a passively pass through cell membrane bilayer.
lipid-soluble substances include;
oxygen, carbon dioxide, nitrogen gases, fatty acids, steroids, fat-soluble vitamins (A, E, D, K), polar molecules (water, and urea)

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9
Q

What is Facilitated Diffusion? What substances are involved with this process?

A
  • require integral membrane proteins to enter cell
  • can form channels called ion channels
  • mostly found; k, cl,
    fewer channels are; Na, and Ca,
    -contain gates
    -others function as carriers bind to 1 side of cell then get released into the opposite side. Includes glucose, galactose, and fructose.
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10
Q

what is a concentration gradient? How does it work?

A
  • difference in concentration btw 2 areas
  • down gradient means, the concentration moves from high to low
  • up gradient means, the concentration moves from low to high
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11
Q

Define solute and Solvent. Then explain concentration.

A

solute - material dissolved in a solution
solvent - substance that dissolves a solute
concentration - amount of solute in a solution. more solute = more concentration.

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12
Q

what is osmotic pressure?

A

solutes particles that can’t pass through cell membrane exert pressure on the cell membrane.
The higher the concentration of solute in a cell the greater the osmotic pressure.

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13
Q

What are the different solutions based on osmotic pressure?

A

Isotonic Solution: any solution where cells maintain their normal shape and volume inside and outside of cells have even solute distribution.
Hypotonic solution: a solution contains lower concentration of solutes (higher concentration of water) the cell will burst due to internal environment of cell having a more concentrated solution
-in red blood cells, this is called hemolysis.
Hypertonic solution: a solution contains a higher concentration of solutes (lower concentration of water) causing the cell to shrink, due to internal environment of cell having a less concentrated solution.
-in red blood cells, this is called Crenation

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14
Q

What is active Transport?

A

is an energy-requiring process that moves solutes such as ions, amino acids, and monosaccharides against a concentration gradient.

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15
Q

What is Osmotic Transport?

A

Osmosis:

  • water molecules move down a concentration gradient.
  • move from area of low solute concentration to high solute concentration.
  • pass through lipid bilayer or integral proteins as water channels.
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16
Q

Define and contrast the terms diffusion, osmosis, isotonic, hypertonic, hypotonic, and facilitated diffusion.

A

Diffusion: When solutions of a high concentration move to an area of low concentration. gradually create equilibrium
Osmosis: water moves from an area of high concentration to an area of low concentration, balancing solutes.
Isotonic: A cell maintains its shape and volume because the solutions are equal to the internal and external parts of the cell.
Hypertonic: The extracellular fluid is more concentrated than internal fluid of cell causing fluid to rush out of the internal cell, causing shrinkage
Hypotonic: the extracellular fluid is less concentrated than the internal fluid of the cell causing fluid to rush into the cell, causing a rupture.
Facilitated Diffusion: require channels or carriers to enter into the cell. they still flow down the concentration gradient.

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17
Q

Define the terms active transport, endocytosis, and exocytosis.

A

Active Transport: cellular energy is used to transport substances across the cell membrane. Moves up the concentration gradient.

  • Endocytosis: molecules or particles that are too large to enter the cell by diffusion or active transport are brought into a vesicle formed from a section of the cell membrane
  • Exocytosis: materials move out of a cell by the fusion of a vesicle formed inside a cell with a plasma membrane.
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18
Q

Distinguish between endocytosis, phagocytosis, pinocytosis, and exocytosis.

A

Endocytosis: a substance that comes into a cell in a vesicle formed by the plasma membrane
Phagocytosis: type of endocytosis where large solid particles are taken in by the cell. (bacteria, viruses, aged/dead cells)
Pinocytosis: another type of endocytosis where cells take up tiny droplets of extracellular fluid
Exocytosis: This process results in the secretions of the cell. Getting rid of materials from the inside of the cell. `

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19
Q

Define cytoplasm

A

The cytoplasm consists of cytosol and organelles.

Cytosol, the intracellular fluid, is the semifluid portion of the cytoplasm that contains inclusions and dissolved solutes.

Cytosol is composed mostly of water, plus proteins, carbohydrates, lipids, and inorganic substances.

The chemicals in cytosol are either in solution or in a colloidal (suspended) form. Functionally, the cytosol is the medium in which many metabolic reactions occur.

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20
Q

List each cellular organelle.

A
Cytoskeleton
Centrosome
Cilia and Flagella
Ribosomes
Endoplasmic Reticulum
Golgi Complex
Lysosomes
Peroxisomes
Proteasomes
Mitochondria
21
Q

Describe the structure and function of the Centrosomes

A

Structure:
-composed of centrioles and pericentriolar matrix
-the 2 centrioles are perpendicular, cylindrical structures, made of 9 clusters of 3 microtubules arranged in a circular pattern,
-surrounding the centrioles is the pericentriolar matrix which contains hundreds of ring-shaped proteins called tubulins.
Function: organizing center for microtubules and mitotic spindle
More specifically; the pericentriolar matrix of the centrosomes contain tubulins that bind microtubules in nondividing cells and form the mitotic spindle during cell division

22
Q

What is the cytoskeleton and what are the different protein filaments?

A

is a network of several kinds of protein filaments that extend throughout the cytoplasm and provide a structural framework for the cell. extend throughout the cytosol.
its function is related mainly to movement and support.

1) Microfilaments:
- thinnest element found in cytoskeleton
- concentrated at the periphery of cell
- contribute to cells strength and shape

2) Intermediate Filaments:
- found in part of cells subject to tension.
- hold organelles in place
- help attach cells to one another

3) Microtubules
-long hollow tubes
are composed of a protein called tubulin, and help determine cell shape and function in the intracellular transport of organelles and the migration of chromosomes during cell division.

23
Q

Describe the structure and function of Cilia and Flagella

A

Structure:
Motile cell surface projections with an inner core of microtubules
Cilia:
-outside the cell form hairlike structures that come out from the surface of cells.
-inside the body, they propel fluids across the surfaces of cells that are firmly anchored.
Flagella:
-similar structure to the cilia but much longer.
-these move the entire cell.

24
Q

Describe the structure and function of Ribosomes

A

Structure:
Ribosomes are small globular granules (about 25 nm across) made of ribosomal RNA and protein. They are found free in the cytoplasm or attached to the endoplasmic reticulum.
(Page 51)
Function:
-Protein Synthesis.
-ribosomes associated the endoplasmic reticulum synthesize proteins destined for insertion in the plasma membrane or secretion from the cell
-free ribosomes synthesize proteins used in the cytosol.

25
Q

Describe the structure and function of the Endoplasmic Reticulum.

A

Structure:
-Membranous network of folded membranes
-rough ER is studded with ribosomes and attached to the nuclear membrane
-smooth ER lacks ribosomes
Function:
-Rough ER synthesizes glycoproteins and phospholipids
-smooth ER is the site of fatty acid and steroid synthesis
-smooth ER releases glucose into the blood
-inactivates or detoxifies drugs and other potentially harmful substances,
-stores and releases calcium ions for muscle contraction.

26
Q

Describe the structure and function of Golgi Complex

A

Structure:
-consists of 3-20 cisterns (flattened membranous sacs with bulging edges piled on each other like a stack of pita bread.)
Function:
-accepts proteins from rough ER
-forms glycoproteins and lipoproteins
-modifies, sorts, packages, and transports proteins from the rough ER

27
Q

Describe the structure and function of Lysosomes

A

Structure:
-membrane-enclosed sacs formed from Golgi complex
-contain as many as 60 different digestive enzymes
Function:
-break down molecules through endocytosis
-transport final digestive products, (monosaccharides, fatty acids, amino acids), to the cytosol
-help recycle worn-out structures (autophagy)
-can destroy the entire cell (autolysis)

28
Q

What is autophagy

A

When a worn-out organelle is ingested and recycled by the lysosomes.

29
Q

What is autolysis

A

when lysosomal enzymes destroy the entire cell.

30
Q

Describe the structure and function of Peroxisomes

A
Structure:
-Similar to lysosomes but are smaller. 
-contain several oxidases, 
Function:
-removes hydrogen from various organic substances. 
-enzymes oxidize toxic substances 
-decomposes hydrogen peroxide 
-enzymes destroy superoxide
31
Q

Describe the structure and function of Proteasomes

A

Structure:
-form a tiny barrel-shaped structure
Function:
-waste unneeded, damaged, or faulty proteins
-breaks proteins up into amino acids
-recycles old proteins into new proteins.

32
Q

Describe the structure and function of the Mitochondria

A

Structure:
-consists of 2 membranes outer and inner
-outer is smooth
-inner membrane is arranged in a series of folds called cristae
-mitochondrial matrix: central fluid-filled cavity
Function:
-generate ATP through reactions of aerobic cellular respiration.

33
Q

Describe the structure and general function of the nucleus.

A

Structure:
-circular/oval shape
-nuclear envelope: separates the nucleus from the cytoplasm
-nuclear pores: pierce the outside of the nuclear envelope
-Nucleolus: inside the nucleus are 1 or more spherical bodies (nucleoli pleural)
-Chromatin: Chromosomes which make up the inner workings of the cell.
Function:
-controls cellular structure
-directs cellular activities
-produces ribosomes in nucleoli

34
Q

Describe the sequence of events involved in protein synthesis.

A

1) Transcription: occurs in the nucleus this is where DNA is copied by RNA (codon), this is catalyzed by the enzyme polymerase. It starts at the promotor and ends at the terminator. Then RNA polymerase, mRNA, rRNA, and tRNA leave the nucleus to patriciate in the next step
2) Translation: occurs in the cytoplasm
- initiator tRNA attaches to a start codon
- large a small ribosomal subunits join to form a functional ribosome and initiator tRNA fits into P site
- anticodon of incoming tRNA Pairs with new mRNA codon at A site
- amino acid on tRNA at P site forms a peptide bond with the amino acid at A site
- the 2-peptide protein creased from the formation of the peptide bond becomes attached to tRNA at A site
- Ribosome shifts by 1 codon: tRNA previously at P site energy E site and is released from ribosomes; tRNA previously at A site is now at the P site
- Protein synthesis stops with the ribosome reaches stop codon on mRNA.
- then the cycle repeats.

35
Q

What is a base Triplet

A

3 consecutive DNA nucleotides

36
Q

What is a codon

A

3 successive RNA nucleotides

37
Q

What is messenger RNA (mRNA)

A

directs synthesis of a protein

38
Q

What is Ribosomal RNA (rRNA)

A

joins with ribosomal proteins to make ribosomes This makes up the small subunit of the ribosome in the cytosol

39
Q

What is Transfer RNA (tRNA)

A

binds to an amino acid and holds it in place on a ribosome until it is incorporated into a protein during translation. This is what gets used during translation in the cytosol.

40
Q

Discuss the stages, events, and significance of somatic cell division.

A

1) Interphase: Replicates DNA and manufactures additional organelles and cytosolic components
2) Mitotic Phase divided into 4 parts:
-early Prophase: condense into chromosomes each with a double-stranded chromatid
-late Prophase: the pericentriolar matrix starts to form an assembly of microtubules called the mitotic spindle, which move to opposite ends of cell
-metaphase: the centromeres of the chromatid pairs are aligned along the microtubules of the mitotic spindle at the exact center of the centromeres
-Anaphase: centromeres split, separating the 2 members of each chromatid pair moving to the opposite ends of the cell. Once separated they are called chromosomes.
-Telophase: when chromosomal movement stops, they uncoil and revert to the threadlike chromatin form. a new nuclear envelop forms around each chromatin mass, nucleoli appear, and the mitotic spindle breaks up.
Cytokinesis: division of the cytoplasm and organelle of a cell this usually begins late anaphase. Microfilaments in the cleavage furrow pull the plasma membrane progressively inward, pulling it into 2.

41
Q

What are the principal constituents of the plasma membrane?

A

a) Integral protein
b) Channel
c) Carbohydrate
d) Cholesterol
e) Lipid bilayer
f) Peripheral protein
(Pages 41–42; Fig. 3.2)

42
Q

Define the terms transcription and translation.

A

Transcription is the copying of a base sequence of DNA into a base sequence of RNA.

Translation is the use of a base sequence of messenger RNA to assemble a corresponding sequence of amino acids.

(Pages 56–57)

43
Q

What is passive transport?

A

involves movement from a region of high concentration to a region of low concentration without an expenditure of energy.

44
Q

What is the function of microtubules

A
  • provide shape and structural support for cells.
  • provide the mechanism for the movement of cilia and flagella
  • provide the mechanism for the movement of chromosomes during mitosis.
45
Q

What is the anticodon?

A

The anticodon is a sequence of three nucleotides on a transfer RNA molecule, which allows the t-RNA to bind to a specific sequence of three nucleotides on the messenger RNA.
(Page 57)

46
Q

What is a gene? what is it’s function?

A

A gene is a specific sequence of nucleotides in DNA that determines the amino acid sequence of a protein.
(Page 55)

47
Q

Define the terms hypotonic and hypertonic.

A

In a hypotonic solution, a lower concentration of solutes and a higher concentration of water occurs. In contrast, a hypertonic solution has a higher concentration of solutes and a lower concentration of water.
(Page 45)

48
Q

For successful somatic cell division to occur, the cell must undergo mitosis followed by cytokinesis. Explain these two terms.

A

a) Division of the nucleus
b) Division of the cytoplasm (cytokinesis)
(Page 59)

49
Q

What is a termination (stop) codon?

A

A termination codon
is a three-nucleotide sequence in messenger RNA that signifies the end of the message.
(Pages 57; Fig. 3.20)