UNIT 2.7

Question 1

wild strains bacteria can be improved by

Answer 1

mutagenesis
selective breeding
recombinant dna

Question 2

mutagenesis is

Answer 2

eliciting mutations with the use of radiation or mutagenic chemicals

Question 3

describe recombinant dne tech as a

Answer 3

dna molecules used to carry forge in genetic material between species

Question 4

recombinant plasmids and artificial chromosomes can be used as

Answer 4

vectors

Question 5

restriction endonuclease are enzymes that are used to

Answer 5

cut open plasmid and specific genes out of chromosomes
restriction endo n cut the dna at a target sequence often leaving sticky ends
the same REN is used to cut out specific genes of interest and the plasmid as they will produce complimentary sticky ends

Question 6

ligase enzyme is used to seal

Answer 6

the genes into the plasmid by attaching the complimentary sticky ends

Question 7

sticky ends

Answer 7

proporties at the end of dna molecules, SE are generated by restriction enzymes that break the molecules

Question 8

describe different sections in recombinant plasmids and artificial chromosomes specifically

Answer 8

• restriction site, contains a target sequence where specific endonuclease cut
• regulatory sequences, controls expression of target gene
• origin of replication, allows plasmid/artificial chromosomes to be replicated
• selectable markers, allows only micro-organisms that have taken up vector to be cultured