Unit 2 Flashcards
Define a cell.
The fundamental unit of life.
What two ways do we study cells?
Microscopy
AND
Cell fractionation
What three things does the quality of a microscopy image depend on? Define each on.
- Magnification-ratio of an object to its size
- Resolution-measure of clarity; minimum distance of 2 distinguishable points
- Contrast- visible differences in parts of a sample
Describe the functions of a light microscope.
- Light passes through specimin>passes through lens system to magnify image
- Magnifies approx. 1000x
- Has various techniques to enhance contrast
- Can’t resolve most sub cellular structures
What are the two types of electron microscopes and describe how they work.
- Scanning electron microscope- focus beam of e- onto the surface of a specimen > 3D image> specimen must be coated in a film of gold
- Transmission Electron Microscope- focus beam of e- through a specimen> 2D image>specimen covered w/ heavy metal
Define cell fractionation.
the act of taking apart and separating cells (separating major organelles)
What four features are common in all cells?
They all have:
- Plasma membrane
- Cytosol
- Chromosomes
- Ribosomes
Describe a prokaryotic cell.
Has no nucleus, (the DNA is located in the nucleoid), no membrane bound organelles, and cytoplasm bound by the plasma membrane
Describe a eukaryotic cell.
The DNA is in a nucleus bound by a membrane envelope, membrane bound organelles, cytoplasm is the region between plasma membrane & nucleus
What are the two groups of organelles?
Endomembrane system and Energy conversion
Define the endomembrane system.
A system that consists of membranes that are directly/indirectly connected by transport vesicles.
What organelles are apart of the endomembrane system and what are their functions?
- Nucleus- information center
- Endoplasmic reticulum- factory
- Golgi apparatus- shipping and receiving
- Lysosomes- digestive compartments- dissassembly components
- Vacuoles- maintenance & storage compartments
What organelles take part in energy conversion and what are their functions?
- Mitochondria- energy production (have their own DNA)
- Chloroplast- make sugars small carbohydrates
- Peroxisome- breakdown compartment
Describe the nuclear envelope.
A double membrane (two lipid bilayers) that encloses the nucleus
What is the function of the nuclear pores on a nucleus?
To regulate entry and exit from nucleus
What is the nuclear lamina?
A protein filament that maintains the shape of the nucleus
Define chromatin.
The genetic material formed by the nucleus DNA & associated proteins. Which condenses to form chromosomes.
Define the nucleolus and its location.
The site of ribosomal RNA (rRNA) synthesis, located within the nucleus.
Describe the function of ribosomes and the two locations they are located.
They cary out protein synthesis in two locations in the
- Cytosol- proteins for use in the cytosol on the
- ER- proteins for use elsewhere
Describe the smooth er and its function.
- Lacks ribosomes
- Synthesizes lipids
- Metabolizes carbs
- Detoxifies poisons
- Stores calcium
Describe the rough er and its function.
Ribosomes on the surface secrete glycoproteins and distributes transport vesicles membrane factory for cell
The golgi apparatus consists of flattened membranous sacs called ____
cisternae
What are the functors of the golgi apparatus?
Modify products of ER
Manufacture some macromolecules sort and package materials into transport vesicles
Membranous sacs of hydrolytic enzymes that digest all four classes of biological macromolecules: ?
lysosome
Describe phagocytosis.
when a cell engulfs another cell
Describe autophagy.
Site of recycling organelles in a cell
Describe vacuoles and their function
- The container for cells
- Food vacuoles are found by phagocytosis
- Contractile vacuoles found in many freshwater protists
- Central vacuoles are found in mature plants; hold water and organic molecules
Which two organelles have a double membrane and their own DNA.
Mitochondria and Chloroplasts
Describe a mitochondria.
Has a smooth outer membrane
Highly folded inner membrane- folded into cristae inner membrane separates inter membrane space and mitochondrial matrix
Describe chloroplasts.
member of the family plastids contain green pigment chlorophyl and molecules that function in photosynthesis CO2 —> small carb Found in leaves and other green organs of plants
What is the structure of a chloroplast?
It contains thylakoids- stacks of membranous sacs called GRANUM as well as storma- internal fluid
What are peroxisomes?
Specialized metabolic compartments bound by single membrane. Use oxygen to break down molecules
Define cytoskeleton.
A network of fibers extending throughout the cytoplasm it organizes the cell’s structures and activities
Describe microtubules and state their functions.
- thickest hollow rods
- approx. 25nm
- grow out of centrosome near the nucleus
Function: shaping the cell, move organelles, separating chromosomes
Describe microfilaments and state their functions.
- A.k.a Actin Filaments
- thinnest components
- solid rods
- approx 7nm
Function: cell shape, microvilli of intestinal cells
Describe intermediate filaments and state their functions.
- middle sized
- range from 8-12 nm
- most permanent fixtures of cytoskeleton
function: cell shape, fix organelles in place
What are two things microtubules make?
Cilia and flagella
What are two types of microfilaments and what are their function?
Actin and Myosin cellular motility muscle contraction ameboid movement cytoplasmic streaming