Unit 1.2 Cell Structure And Organisation Flashcards
Microscopes:
Comparing light microscopes to electron microscopes
What is the source of image?
Microscopes:
Comparing light microscopes to electron microscopes
How is the beam focused?
Light microscope -
Use a condenser above the light source
Electron microscope -
Using a magnetic lens
Microscopes:
Comparing light microscopes to electron microscopes
What is the max effect of magnification?
Light microscope -
x1500
Electron microscope -
x100,000 to x300,000
Microscopes:
Comparing light microscopes to electron microscopes
What is the max resolution?
Light microscope -
200nm
Electron microscope -
Around 0.050nm
Microscopes:
Comparing light microscopes to electron microscopes
Can a live specimen be used?
Light microscope -
Living and dead
Electron microscope -
No, the electrons destroy the specimen. Only use dead and dry
Microscopes:
Comparing light microscopes to electron microscopes
Section or external view of specimen?
Light microscope -
Electron microscope -
Microscopes:
Comparing light microscopes to electron microscopes
How much do they cost?
Light microscope -
Electron microscope -
Microscopes:
Comparing light microscopes to electron microscopes
Can the image be viewed directly?
Light microscope -
Yes
Electron microscope -
No, electrons cannot be observed by human eye
Microscopes:
Comparing light microscopes to electron microscopes
Do you need to stain the specimen?
Light microscope -
Yes
Electron microscope -
Yes with heavy metals?
Microscopes:
What’s the definition of magnification?
Making something bigger/enlarging something
Microscopes:
What is the definition of resolution?
How clearly you can see something
Microscopes:
What are the different magnifications?
Eyepiece: x10
Objective: x4
Overall magnification: x40
Eyepiece: x10
Objective: x10
Overall magnification: x100
Eyepiece: x10
Objective: x40
Overall magnification: x400
Eyepiece: x10
Objective: x100
Overall magnification: x1000
Microscopes:
What equation includes image size, actual size and magnification?
Image size = actual size x magnification
Unit conversion:
cm to mm
m to mm
mm to micrometers
Micrometers to nm
cm to mm = x10
m to mm = x1000
mm to micrometers = x1000
Micrometers to nm = x1000
Can you briefly summarise eukaryotic cells?