Unit 1 - Laboratory Techniques for Biologists

Question 1

What is a hazard?

Answer 1

Anything which could cause harm

Question 2

Hazards in a lab examples

Answer 2

• Toxic or corrosive chemicals
• Heat or flammable subtances
• Pathogenic organisms

Question 3

What is a risk?

Answer 3

The likelihood of harm arising from exposure to a hazard

Question 4

What is a risk assessment?

Answer 4

Identifying control measures to minimise risk

Question 5

Control method examples

Answer 5

• Wear PPE
• Use appropriate handling equipment
• Use aseptic techniques

Question 6

Example of linear dilutions

Answer 6

0.1, 0.2, 0.3

Question 7

Example of log dilutions

Answer 7

0.1, 0.01, 0.001

Question 8

What is a blank cuvette used for?

Answer 8

Calibrating the colorimeter, acts as a baseline value for comparison

Question 9

Separation techniques examples

Answer 9

Centrifuge, Chromatography, Gel electrophoresis, Isoelectric point,

Question 10

What is centrifugation?

Answer 10

Allows substances to be separated according to their density

Question 11

In centrifugation, what do dense materials form?

Answer 11

A pellet at the bottom of the tube

Question 12

In centrifugation, what are less dense materials called and where do they go?

Answer 12

Supernatants, remain in the liquid

Question 13

What is chromatography?

Answer 13

Allows components of a mixture to be identified and purified

Question 14

What are the 3 types of chromatography?

Answer 14

• Paper chromatography
• Thin layer chromatography
• Affinity chromatography