UNIT 1: Laboratory Safety

Question 1

T/F:

No food is allowed but drinking is permitted inside the laboratory.

Answer 1

False

Question 3

What is used to clean the working area before and after lab exercises?

Answer 3

1:10 dilution of 5% sodium hypochlorite

Question 4

Any spillage of infectious agents must be flooded and cleaned with : dilution of _% sodium hypochlorite

Answer 4

1:5 dilution of 5% sodium hypochlorite

Question 5

Color:

for infectious and contaminated materials

used cotton, tissue, gloves, culture medium, petri dishes

Answer 5

yellow plastic bag

Question 6

Color:

for noninfectious dry materials

Answer 6

black plastic bag

Question 7

Color:

for noninfectious wet materials

Answer 7

green plastic bag

Question 8

color:

container for contaminated sharps

such as needles

Answer 8

red puncture proof container

Question 9

T/F:

contact lenses are to be worn during bacteriology lab

Answer 9

False.

not advisable as they may absorb volatile fumes

Question 10

If splashes are anticipated while working, what are advisable to be worn?

Answer 10

face shield or laboratory goggles

Question 11

T/F:

shoes worn must be open-toe

Answer 11

False

close toe. should cover the entire foot.

Question 12

Students must handle all specimens with care as p_ h_

Answer 12

potentially hazardous

Question 13

Enumerate:

(5) possible routes of infection in the microbiology laboratory

Answer 13

• airborne
• ingestion
• direct inoculation
• mucous membrane contact
• arthropod vectors

Question 14

what may form during centrifugation of **unstoppered tubes **or from heating cultures too rapidly?

clue: A_

Answer 14

aerosols

Question 15

Route:

may occur as a result of failure to wash hands or pipetting with the mouth

Answer 15

ingestion

Question 16

Route:

infection may result from needlesticks, broken glass, animal bites, or small scratches on fingers

Answer 16

direct inoculation

Question 17

Route:

infection may occur if the organism can directly enter through the mucous membranes

such as the conjunctiva of the eye

Answer 17

mucous membrane contact

Question 18

route:

infectious sources include ticks, fleas, and mosquitoes, which may harbor various microorganisms

Answer 18

arthropod vectors

Question 19

Enumerate:

3 arthropod vectors

Answer 19

• ticks
• fleas
• mosquitoes

Question 20

Orientation of continuing education for employees

Answer 20

Safety Education

Question 21

By CDC (1987)

set of preventive measures designed to reduce the risk of HIV, HBV, and other bloodborne pathogens in the health care setting

Answer 21

Universal Precautions

Question 22

Combination of Universal Precautions and Body Substance Isolation to minimize risk of infection transmission

Answer 22

Standard Precautions

Question 23

T/F:

All blood, tissue, body fluid, secretions and excretions, including sweat, are considered potentially infectious

Answer 23

False

all EXCEPT SWEAT

Question 24

Are devices that isolate or remove a hazard

Answer 24

Engineering Controls

Question 25

A _ is a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents

Answer 25

Biosafety Cabinet (BSC)

Question 26

Enumerate:

In a BSC, air that contains infectious material is sterilized by (3)

Answer 26

• heat
• UV light
• passage through HEPA filter

Question 27

What is the lowest class/level of BSC?

Answer 27

BSC Class 1

Question 28

BSC CLASS

allows unsterilized air to pass into the cabinet, sterilizing only the air to be exhausted

Answer 28

BSC Class 1

Question 29

T/F:

BSC Class 1 prevents contamination of the sample

Answer 29

False

it protects the person

Question 30

What does HEPA Filter stand for?

Answer 30

High Efficiency Particulate Air Filter

Question 31

T/F:

BSC Class 2 prevents contamination of the sample and filters the air

Answer 31

True

Question 32

BSC CLASS

sterilizes air that flows over the infectious material, as well as air to be exhausted

Answer 32

BSC Class 2

Question 33

BSC CLASS 2 TYPE _

self-contained, and 70% of the air is recirculated into the work area

Answer 33

BSC CLASS 2 TYPE A

a = contAined

Question 34

BSC CLASS 2 TYPE _

Discharged outside the building

Answer 34

BSC CLASS 2 TYPE B

b = building

Question 35

T/F:

BSC Class 3 Type C can operate as either a type A when recirculating or as type B when exhausting

Answer 35

False

Class 2 Type C

Question 36

What BSC has the greatest protection?

Answer 36

BSC Class 3

Question 37

Least preferred way to contain a hazard

Answer 37

PPE

best protection: BSC cabinet