UNIT 1: Laboratory Safety Flashcards
T/F:
No food is allowed but drinking is permitted inside the laboratory.
False
What is used to clean the working area before and after lab exercises?
1:10 dilution of 5% sodium hypochlorite
Any spillage of infectious agents must be flooded and cleaned with : dilution of _% sodium hypochlorite
1:5 dilution of 5% sodium hypochlorite
Color:
for infectious and contaminated materials
used cotton, tissue, gloves, culture medium, petri dishes
yellow plastic bag
Color:
for noninfectious dry materials
black plastic bag
Color:
for noninfectious wet materials
green plastic bag
color:
container for contaminated sharps
such as needles
red puncture proof container
T/F:
contact lenses are to be worn during bacteriology lab
False.
not advisable as they may absorb volatile fumes
If splashes are anticipated while working, what are advisable to be worn?
face shield or laboratory goggles
T/F:
shoes worn must be open-toe
False
close toe. should cover the entire foot.
Students must handle all specimens with care as p_ h_
potentially hazardous
Enumerate:
(5) possible routes of infection in the microbiology laboratory
- airborne
- ingestion
- direct inoculation
- mucous membrane contact
- arthropod vectors
what may form during centrifugation of **unstoppered tubes **or from heating cultures too rapidly?
clue: A_
aerosols
Route:
may occur as a result of failure to wash hands or pipetting with the mouth
ingestion
Route:
infection may result from needlesticks, broken glass, animal bites, or small scratches on fingers
direct inoculation
Route:
infection may occur if the organism can directly enter through the mucous membranes
such as the conjunctiva of the eye
mucous membrane contact
route:
infectious sources include ticks, fleas, and mosquitoes, which may harbor various microorganisms
arthropod vectors
Enumerate:
3 arthropod vectors
- ticks
- fleas
- mosquitoes
Orientation of continuing education for employees
Safety Education
By CDC (1987)
set of preventive measures designed to reduce the risk of HIV, HBV, and other bloodborne pathogens in the health care setting
Universal Precautions
Combination of Universal Precautions and Body Substance Isolation to minimize risk of infection transmission
Standard Precautions
T/F:
All blood, tissue, body fluid, secretions and excretions, including sweat, are considered potentially infectious
False
all EXCEPT SWEAT
Are devices that isolate or remove a hazard
Engineering Controls
A _ is a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents
Biosafety Cabinet (BSC)
Enumerate:
In a BSC, air that contains infectious material is sterilized by (3)
- heat
- UV light
- passage through HEPA filter
What is the lowest class/level of BSC?
BSC Class 1
BSC CLASS
allows unsterilized air to pass into the cabinet, sterilizing only the air to be exhausted
BSC Class 1
T/F:
BSC Class 1 prevents contamination of the sample
False
it protects the person
What does HEPA Filter stand for?
High Efficiency Particulate Air Filter
T/F:
BSC Class 2 prevents contamination of the sample and filters the air
True
BSC CLASS
sterilizes air that flows over the infectious material, as well as air to be exhausted
BSC Class 2
BSC CLASS 2 TYPE _
self-contained, and 70% of the air is recirculated into the work area
BSC CLASS 2 TYPE A
a = contAined
BSC CLASS 2 TYPE _
Discharged outside the building
BSC CLASS 2 TYPE B
b = building
T/F:
BSC Class 3 Type C can operate as either a type A when recirculating or as type B when exhausting
False
Class 2 Type C
Least preferred way to contain a hazard
PPE
best protection: BSC cabinet
