UNIT 1: Laboratory Safety Flashcards
T/F:
No food is allowed but drinking is permitted inside the laboratory.
False
What is used to clean the working area before and after lab exercises?
1:10 dilution of 5% sodium hypochlorite
Any spillage of infectious agents must be flooded and cleaned with : dilution of _% sodium hypochlorite
1:5 dilution of 5% sodium hypochlorite
Color:
for infectious and contaminated materials
used cotton, tissue, gloves, culture medium, petri dishes
yellow plastic bag
Color:
for noninfectious dry materials
black plastic bag
Color:
for noninfectious wet materials
green plastic bag
color:
container for contaminated sharps
such as needles
red puncture proof container
T/F:
contact lenses are to be worn during bacteriology lab
False.
not advisable as they may absorb volatile fumes
If splashes are anticipated while working, what are advisable to be worn?
face shield or laboratory goggles
T/F:
shoes worn must be open-toe
False
close toe. should cover the entire foot.
Students must handle all specimens with care as p_ h_
potentially hazardous
Enumerate:
(5) possible routes of infection in the microbiology laboratory
- airborne
- ingestion
- direct inoculation
- mucous membrane contact
- arthropod vectors
what may form during centrifugation of **unstoppered tubes **or from heating cultures too rapidly?
clue: A_
aerosols
Route:
may occur as a result of failure to wash hands or pipetting with the mouth
ingestion
Route:
infection may result from needlesticks, broken glass, animal bites, or small scratches on fingers
direct inoculation
Route:
infection may occur if the organism can directly enter through the mucous membranes
such as the conjunctiva of the eye
mucous membrane contact
route:
infectious sources include ticks, fleas, and mosquitoes, which may harbor various microorganisms
arthropod vectors
Enumerate:
3 arthropod vectors
- ticks
- fleas
- mosquitoes
Orientation of continuing education for employees
Safety Education
By CDC (1987)
set of preventive measures designed to reduce the risk of HIV, HBV, and other bloodborne pathogens in the health care setting
Universal Precautions
Combination of Universal Precautions and Body Substance Isolation to minimize risk of infection transmission
Standard Precautions
T/F:
All blood, tissue, body fluid, secretions and excretions, including sweat, are considered potentially infectious
False
all EXCEPT SWEAT
Are devices that isolate or remove a hazard
Engineering Controls
