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BACTE LAB > COLONIAL MORPHOLOGY > Flashcards

COLONIAL MORPHOLOGY Flashcards

1
Q

QUALI OR QUANTI

what type of isolation is used for urine culture?

A

quantitative

because you do COLONY COUNTING

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2
Q

T/F:

Hemolysis can be seen on both Blood Agar and Choco Agar plates

A

False

BAP only. Choco agar RBC are already lysed while heating

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3
Q

Enumerate:

3 types of hemolysis in BAP

A
  • alpha
  • beta
  • gamma
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4
Q

hemolysis

partial; greenish zone

A

alpha hemolysis

example: S. pneumoniae

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5
Q

hemolysis

complete; clear zone

A

beta hemolysis

examples: S. pyogenes, S. aureus

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6
Q

hemolysis

no hemolysis

A

gamma hemolysis

example: E. faecalis

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7
Q

Score of a plate with colonies visible only in quadrant 1, with fewer than 10 colonies?

A

1+

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8
Q

score of a plate with colonies visible in quadrants 1 and 2, with more than 10 colonies in quadrant 1 and fewer than 10 in quadrant 2?

A

2+

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9
Q

If colonies are present in quadrants 1, 2, and 3, and more than 10 colonies are observed in quadrants 1 and 2 but fewer than 10 in quadrant 3, what is the score?

A

3+

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10
Q

A plate shows colony growth in all four quadrants, with more than 10 colonies in each quadrant, what is the score?

A

4+

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11
Q

Give an example of Double Zone of Hemolysis

A

C. perfringens

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12
Q

enumerate

sizes of colonical morphology (3)

A
  • pinhead
  • pinpoint
  • large, medium
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13
Q

sizes

typical of staphylococci

A

pinhead

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14
Q

sizes

typical of streptococci

A

pinpoint

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15
Q

sizes

tend to be G(-) bacilli

A

large, medium

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16
Q

enumerate

colony shapes

A
  • punctiform (pinpoint)
  • circular
  • filamentous
  • irregular
  • rhizoid
  • curled
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17
Q

enumerate

elevation

A
  • flat
  • raised
  • convex (dome)
  • umbilicate
  • umbonate
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18
Q

enumerate

margins

A
  • entire
  • undulate
  • filamentous
  • lobate
  • erose
  • curled
  • scalloped
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19
Q

dry, wrinkled, breadcrumb-like

A

Nocardia spp.

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20
Q

hammered copper

A

Yersinia pestis

aka plague bacilli

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21
Q

identify

what bacteria shows a green, pearlescent appearance on a BAP?

A

P. aeruginosa

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22
Q

Enumerate

pigments produced by Pseudomonas (4)

A
  • pyoverdine
  • pyocyanin
  • pyorubin
  • pyomelanin
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23
Q

color

pyoverdine

A

green

though **yellow-green **yung sabi ni pubmed

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24
Q

color

pyocyanin

A

blue

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25
Q

color

pyorubin

A

red

red-brown sabi ni pubmed

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26
Q

color

pyomelanin

A

black/brown

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27
Q

Pedicle/footlike

A

Candida albicans

this is not a bacteria, it’s YEAST

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28
Q

Density of a colony can be: (3)

A
  • transparent
  • translucent
  • opaque

to see diff densities, look through the colony while using transillumination

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29
Q

This bacteria produces semi-opaque colonies, with organisms concentrated at the center

sometimes called “bull’s eye”

A

Streptococcus agalactiae

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30
Q

T/F:

Staphylococci are usually translucent

A

False

opaque

source: mahon

31
Q

This bacteria is described as shiny (like a half-pearl) on blood-containing media

A

Bordetella pertussis

source: mahon

32
Q

color or pigment

this is used to describe a particular genus in general

A

color

33
Q

color or pigment

an inherent characteristic of a specific organism confined generally to the colony, but will sometimes diffuse through the culture medium

A

pigment

33
Q

color

coagulase negative staphylococci

A

white

34
Q

color

G(-) rods, Enterococcus spp.

A

gray

35
Q

color

S. aureus, Micrococcus spp., non-pathogenic Neisseria spp.

A

yellow or off-white

36
Q

color

diphtheroids

A

buff

37
Q

thioglycolate broth is stored at what temp?

A

room temp

38
Q

liquid media

if the growth is all throughout the broth..

A

facultative

39
Q

liquid media

if the growth is at the top only

A

obligate aerobe

40
Q

ODOR

Proteus

A

burnt chocolate/chocolate cake

41
Q

ODOR

S. aureus

A

old sock

42
Q

ODOR

bleach

A

E. corrodens

eikenella

43
Q

ODOR

corn taco/grapefruit

A

Pseudomonas aeruginosa

44
Q

ODOR

P. multocida

A

musty

45
Q

ODOR

almond like

A

Chromobacterium violaceum

46
Q

ODOR

barnyard

A

C. difficile

47
Q

ODOR

popcorn like

A

Neisseria animaloris

48
Q

ODOR

Nocardia

A

freshly plowed field

49
Q

ODOR

Haemophilus

A

musty basement

50
Q

ODOR

A. faecalis

A

freshly cut apple/strawberry

51
Q

FORM/MARGIN

medusa head

A

Bacillus anthracis

52
Q

FORM/MARGIN

Eikenella corrodens

A

fuzzy edged

53
Q

FORM/MARGIN

Proteus spp.

A

swarmers

54
Q

FORM/MARGIN

wagon-wheel

A

Moraxella catarrhalis

55
Q

FORM/MARGIN

Actinomyces

A

molar-tooth appearance

56
Q

FORM/MARGIN

M. tuberculosis

A

cauliflower-like

57
Q

FORM/MARGIN

C. albicans

A

foot/pedicle

58
Q

FORM/MARGIN

Yersinia pestis

A

stalactite pattern

59
Q

CONSISTENCY

Nocardia spp.

A

brittle (forms splinters; breadcrumb like)

60
Q

CONSISTENCY

S. aureus

A

creamy/buterous

61
Q

CONSISTENCY

Diphtheroids

A

dry and waxy

62
Q

CONSISTENCY

sticky

A

Neisseria spp.

63
Q

CONSISTENCY

beaten egg white

A

B. anthracis

64
Q

CONSISTENCY

mucoid

A

capsulated organisms (Klebsiella pneumoniae)

65
Q

Serratia marcesens is red due to..

A

prodigiosin

66
Q

pigment of C. violaceum

A

purple

67
Q

convex, entire edge, 2-3mm, creamy, yellowish, beta-hemolytic

A

Staphylococcus

basta may creamy, almost always staph:D

68
Q

convex/pulvinate, translucent, pinpoint, butyrous, wide zone of beta-hemolysis

A

Streptococcus

69
Q

umbilicate or flat, translucent, butyrous or mucoid, broad zone of alpha hemolysis

A

Pneumococcus

70
Q

pulvinate, semiopaque, gray, moist to dry, beta-hemolysis may or may not be present

A

E. coli & other enterics

71
Q

flat, gray, spreading as a thin film on agar, **burn chocolate **odor

A

Proteus

72
Q

flat, gray to greenish, margins erose or spreading, green-blue pigment, grapelike odor

A

Pseudomonas

BACTE LAB (5 decks)

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