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BACTE LAB > MICROSCOPY: MORPHOLOGY & ARRANGEMENT > Flashcards

MICROSCOPY: MORPHOLOGY & ARRANGEMENT Flashcards

1
Q

SIMPLE OR DIFFERENTIAL

provides morphology and arrangement

A

simple stain

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2
Q

SIMPLE OR DIFFERENTIAL

provides gram reaction, morphology, and arrangement

A

differential stain

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3
Q

SIMPLE OR DIFFERENTIAL

cocci in chains

A

simple stain

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4
Q

SIMPLE OR DIFFERENTIAL

gram positive cocci in clusters

A

differential stain

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5
Q

DIRECT OR INDIRECT

prepare smear from clinical specimens

A

direct smear

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6
Q

DIRECT OR INDIRECT

prepare smear from culture

A

indirect smear

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7
Q

DIRECT OR INDIRECT

bacterial cells, other cells, and other structures

A

direct smear

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8
Q

DIRECT OR INDIRECT

bacterial cells

A

indirect smear

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9
Q

T/F:

when focusing, focus first under LPO then shift directly to OIO

A

True

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10
Q

this shape is not usually encountered

A

spiral

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11
Q

other name for comma shaped bacilli

A

vibrio

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12
Q

give example/s

gram positive cocci in tetrads

A

Micrococcus tetragena

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13
Q

give example/s

Gram positive cocci in pairs
(coffee-bean shaped)

A

Streptococcus pneumoniae

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14
Q

give example/s

Gram negative cocci in pairs

may be found intracellularly inside PMN

A
  • Neisseria sp.
  • Neisseria meningitidis
  • Neisseria gonorrhoeae
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15
Q

give example/s

gram positive cocci in sarcinae (cubical packet)

A

Sarcina lutea

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16
Q

give example/s

Gram positive cocci in clusters

A

Staphylococcus

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17
Q

give example/s

Gram positive bacilli in chains

large bacilli with square cut ends

A

Bacillus

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18
Q

has a bamboo fishing pole appearance

A

Bacillus

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19
Q

give example/s

gram negative bacilli in pairs

A

non-fermenters such as Pseudomonas

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20
Q

give example/s

Gram negative coccobacilli in pairs, singles

A

Enterics

found in the GIT

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21
Q

give example/s

Gram-positive bacilli that are large, arranged singly or individually with spores

A
  • Clostridium tetani
  • Clostridium botulinum
  • Clostridium perfringens
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22
Q

Clostridium with

“box car appearance”

A

Clostridium perfringens

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23
Q

drumstick bacillus

A

Clostridium tetani

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24
Q

give example/s

gram negative coccobacilli straight and rod shaped

A

Haemophilus ducreyi

this is an STD

25
Q

H. ducreyi has what pattern?

A
  • school of fish
  • railroad track pattern
26
Q

the smallest pathogenic bacilli

0.2 x 0.5 um

A

H. ducreyi

27
Q

give example/s

Gram negative bacilli with rounded ends

A

Bacteroides fragilis

28
Q

This G(+) irregular bacilli is club shaped, Chinese character, X or Y, palisade, snapping arrangement

A

Corynebacterium

29
Q

give example/s

Gram positive branching bacilli with bifurcated/spatulated ends

A

Bifidobacterium

30
Q

give example/s

gram positive slender, branching, filamentous bacilli

A

Nocardia

31
Q

G(+) filamentous bacilli with a v-shaped pattern

A

Erysipelothrix rhusiopathiae

hahaha goodluck sa pagmemorize

32
Q

also called necklace bacillus

A

Streptobacillus moniliformis

33
Q

give example/s

Gram (-) bacilli arranged in long looped filamentous forms

A

Streptobacillus moniliformis

34
Q

give example/s

Gram (-) fusiform bacilli

A

Fusobacterium nucleatum

35
Q

give example/s

Gram (-) comma shaped bacilli in singles

A

Vibrio

36
Q

give example/s

Gram (-) seagull wing shaped, S shaped bacilli

A

Campylobacter

37
Q

give example/s

Gram (-) curved bacilli with two or more curves

A

Spirillum

38
Q

causative agent of red disease

A

Erysipelothrix rhusiopathiae

39
Q

Primary stain in acid fast bacilli

A

Carbol fuchsin

40
Q

mordant in AFB

A

heat or tergitol

41
Q

give example/s

AFB slender, long, arranged in serpentine cord like pattern

A

M. tuberculosis

42
Q

give example/s

AFB slender and in palisades/globi packets/cigar bundle; arranged singly

A

Mycobacterium leprae

43
Q

causative agent of leprosy

A

Mycobacterium leprae

44
Q

what type of microscope is used in viewing spirochetes?

A

dark field microscope

45
Q

spirochetes

tightly twisted; with one end or both bent to a hook

A

Leptospira interrogans

46
Q

spirochetes

tightly twisted (corkscrew)

A

Treponema pallidum

47
Q

spirochete

flexibly twisted; can be stained with Giemsa

A

Borrelia

48
Q

this is the only spirochete that can be stained

A

Borrelia

49
Q

T/F:

Pseudohypha are bacteria

A

False

50
Q

2 not medically important shapes

A
  • square shaped
  • star shaped
51
Q

shape of

Stella spp.

A

star shaped

52
Q

shape of

Haloarcula spp.

A

square shape

53
Q

AFB bacilli typically appear what color?

A

red

54
Q

DSSM stands for..

A

Direct Sputum Smear Microscopy

55
Q

_ OIF should be read before reporting the sputum smear as negative for AFB

A

300 OIF

56
Q

DSSM

reading/scanning of the smear under OIO is done in a _ manner

A

systematic

57
Q

structures seen in a sputum smear

A
  • WBCs (pus cells)
  • squamous epithelial cells
  • bacteria present: cocci in pairs
  • mucus threads
  • curschmann’s spirals
58
Q

spiral shaped structures that can be seen in asthmatics

A

CURSCHMANN’S SPIRALS

BACTE LAB (5 decks)

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