MICROSCOPY: MORPHOLOGY & ARRANGEMENT

Question 1

SIMPLE OR DIFFERENTIAL

provides morphology and arrangement

Answer 1

simple stain

Question 2

SIMPLE OR DIFFERENTIAL

provides gram reaction, morphology, and arrangement

Answer 2

differential stain

Question 3

SIMPLE OR DIFFERENTIAL

cocci in chains

Answer 3

simple stain

Question 4

SIMPLE OR DIFFERENTIAL

gram positive cocci in clusters

Answer 4

differential stain

Question 5

DIRECT OR INDIRECT

prepare smear from clinical specimens

Answer 5

direct smear

Question 6

DIRECT OR INDIRECT

prepare smear from culture

Answer 6

indirect smear

Question 7

DIRECT OR INDIRECT

bacterial cells, other cells, and other structures

Answer 7

direct smear

Question 8

DIRECT OR INDIRECT

bacterial cells

Answer 8

indirect smear

Question 9

T/F:

when focusing, focus first under LPO then shift directly to OIO

Answer 9

True

Question 10

this shape is not usually encountered

Answer 10

spiral

Question 11

other name for comma shaped bacilli

Answer 11

vibrio

Question 12

give example/s

gram positive cocci in tetrads

Answer 12

Micrococcus tetragena

Question 13

give example/s

Gram positive cocci in pairs
(coffee-bean shaped)

Answer 13

Streptococcus pneumoniae

Question 14

give example/s

Gram negative cocci in pairs

may be found intracellularly inside PMN

Answer 14

• Neisseria sp.
• Neisseria meningitidis
• Neisseria gonorrhoeae

Question 15

give example/s

gram positive cocci in sarcinae (cubical packet)

Answer 15

Sarcina lutea

Question 16

give example/s

Gram positive cocci in clusters

Answer 16

Staphylococcus

Question 17

give example/s

Gram positive bacilli in chains

large bacilli with square cut ends

Answer 17

Bacillus

Question 18

has a bamboo fishing pole appearance

Answer 18

Bacillus

Question 19

give example/s

gram negative bacilli in pairs

Answer 19

non-fermenters such as Pseudomonas

Question 20

give example/s

Gram negative coccobacilli in pairs, singles

Answer 20

Enterics

found in the GIT

Question 21

give example/s

Gram-positive bacilli that are large, arranged singly or individually with spores

Answer 21

• Clostridium tetani
• Clostridium botulinum
• Clostridium perfringens

Question 22

Clostridium with

“box car appearance”

Answer 22

Clostridium perfringens

Question 23

drumstick bacillus

Answer 23

Clostridium tetani

Question 24

give example/s

gram negative coccobacilli straight and rod shaped

Answer 24

Haemophilus ducreyi

this is an STD

Question 25

H. ducreyi has what pattern?

Answer 25

* school of fish * railroad track pattern

Question 26

the smallest pathogenic bacilli | 0.2 x 0.5 um

Answer 26

*H. ducreyi*

Question 27

# give example/s Gram negative bacilli with rounded ends

Answer 27

*Bacteroides fragilis*

Question 28

This G(+) irregular bacilli is **club shaped**, Chinese character, X or Y, palisade, snapping arrangement

Answer 28

*Corynebacterium*

Question 29

# give example/s Gram positive **branching** bacilli with **bifurcated**/spatulated ends

Answer 29

*Bifidobacterium*

Question 30

# give example/s gram positive **slender**, **branching**, **filamentous** bacilli

Answer 30

***Nocardia***

Question 31

G(+) filamentous bacilli with a **v-shaped pattern**

Answer 31

*Erysipelothrix rhusiopathiae* ## Footnote hahaha goodluck sa pagmemorize

Question 32

also called **necklace bacillus**

Answer 32

Streptobacillus moniliformis

Question 33

# give example/s Gram (-) bacilli arranged in long **looped** filamentous forms

Answer 33

*Streptobacillus moniliformis*

Question 34

# give example/s Gram (-) fusiform bacilli

Answer 34

*Fusobacterium nucleatum*

Question 35

# give example/s Gram (-) **comma** shaped bacilli in singles

Answer 35

*Vibrio*

Question 36

# give example/s Gram (-) **seagull wing** shaped, S shaped bacilli

Answer 36

*Campylobacter*

Question 37

# give example/s Gram (-) curved bacilli with **two or more curves**

Answer 37

*Spirillum*

Question 38

causative agent of **red disease**

Answer 38

*Erysipelothrix rhusiopathiae*

Question 39

Primary stain in acid fast bacilli

Answer 39

**Carbol fuchsin**

Question 40

mordant in AFB

Answer 40

heat or tergitol

Question 41

# give example/s AFB slender, long, arranged in **serpentine cord like** pattern

Answer 41

*M. tuberculosis*

Question 42

# give example/s AFB slender and in palisades/globi packets/**cigar bundle;** arranged singly

Answer 42

*Mycobacterium leprae*

Question 43

causative agent of leprosy

Answer 43

*Mycobacterium leprae*

Question 44

what type of microscope is used in viewing spirochetes?

Answer 44

dark field microscope

Question 45

# spirochetes **tightly twisted;** with one end or both bent to a **hook**

Answer 45

*Leptospira interrogans*

Question 46

# spirochetes tightly twisted (**corkscrew**)

Answer 46

*Treponema pallidum*

Question 47

# spirochete flexibly twisted; can be stained with Giemsa

Answer 47

*Borrelia*

Question 48

this is the only spirochete that can be stained

Answer 48

*Borrelia*

Question 49

# T/F: Pseudohypha are bacteria

Answer 49

False

Question 50

2 not medically important shapes

Answer 50

* square shaped * star shaped

Question 51

# shape of *Stella spp.*

Answer 51

star shaped

Question 52

# shape of *Haloarcula spp.*

Answer 52

square shape

Question 53

AFB bacilli typically appear what color?

Answer 53

red

Question 54

DSSM stands for..

Answer 54

Direct Sputum Smear Microscopy

Question 55

_ OIF should be read before reporting the sputum smear as negative for AFB

Answer 55

300 OIF

Question 56

# DSSM reading/scanning of the smear under OIO is done in a _ manner

Answer 56

systematic

Question 57

structures seen in a sputum smear

Answer 57

* WBCs (pus cells) * squamous epithelial cells * bacteria present: cocci in pairs * mucus threads * curschmann's spirals

Question 58

spiral shaped structures that can be seen in asthmatics

Answer 58

CURSCHMANN'S SPIRALS