Unit 1 - Introduction to the Cell Flashcards
Transmission Emission Microscopy
looks at thin sections of tissues - the ‘guts’ of the cell
Fluorescence Microscopy
illumination and electronic image processing, sees cell components in finer detail
Yeast (Saccharomyces cerevisiae)
cell cycle/mitosis
worm - C. elegans
first animal genome to be sequenced
E.coli
DNA replication
Evidence of endosymbiont theory
M & C …
- similar size to bacteria
- double membranes
- own ribosomes
- own genomes
- genetically-similar to ‘parent’ bacteria as apposed to eukaryotic cells
Endosymbiont Theory
- early eukaryotes originated as predators
- mitochondria and chloroplasts evolved from smaller prokaryotes that were engulfed by larger cells
Mitochondria
generates energy to power cell
Golgi
collection, packaging and distribution
Rough ER
synthesis of proteins for export, insertion into membranes, lysosomes
Nucleus
stores chromosomes, DNA
Animals
- Multicellular
- Heterotrophs
- No cell walls
Plants
- Autotrophs - self-feeding
- cell walls
- multicellular
Fungi
- single celled or multi cellular
- cell walls
- heterotrophs - dependent on external source of organic compounds
Protists
- single celled
- most diverse group
- much larger compared to bacteria
Prokaryotic Cell Shapes
- Rod-like
- Spherical
- Corkscrew
Generic Prokaryotic Cell
- No membrane-bound nucleus
- DNA is a single strand, circular and free-floating
- Most have a cell wall in addition to the plasma membrane
- Ribosomes, plasmids, cytoplasm, chromosomes, flagellum (occasionally)
archeabacteria
have cell walls
(eu)bacteria
have cell walls (exception: mycoplasma)
Functions of All Cells
- Acquire and use energy
- Carry out a variety of chemical reactions (cellular metabolism)
- Engage in mechanical activities (transport, assembly/disassembly, movement)
- Respond to signals
The Cell Theory
- All living organisms are composed of cells
- The cell is the basic unit of structure for all organisms
- All cells arise from pre-existing cells
Theodore Schwann
1839, similar aspects observed in animal cells
Matthias Schleiden
Schleiden
1838, embryonic plants arose from single cell, all plant tissue composed of cells
Robert Brown
1833, noticed every plant cell had a ‘kernel’ - later names nucleus
Antonie van Leeuwenhoek
1632-1723
- worked with glass to improve magnification abilities
- first to observe: protists of pond water, bacteria from mouth, sperm, red blood cells, banded pattern in muscle cells, -labelled “Father of Microbiology”
Robert Hooke
1635-1703
- first microscope
- viewed slices of cork through microscope - said they looked like tiny little rooms
Potential Energy (in chemical reactions)
making and breaking of chemical bonds
shifting of atoms from one molecule to another
starch
- storage form of energy in animals
- consists of alpha glucose monomers linked by alpha 1,4 bonds (unbranched) and branched alpha 1,6 and 1,4 bonds
Scanning Electron Microscopy
looks at surface details of cell/other structures
Cytology
light microscopy, can see tissues clearly, ink dyes can be used to view different components of the cell
Mouse
genetics well understood