Unit 1/3- infectious disease Flashcards
What is the equation for magnification?
Eye piece lens x Objective lens
What are some of the risks of the cheek cell experiment?
Glass, disinfectant, stain, microscope, cotton bud
Describe the process of the cheek cell experiment
Sterile cotton bud inside cheek, smear over a slide, stain, cover slip at 90 degrees, under microscope
Give advantages of a light microscope
Portable, living or dead, inexpensive, simple sample preperation
Give disadvantages of a light microscope
Specifics cant be seen
Maximum magnification and resolution of a light microscope?
X2000 and 200nm
Slide prep process?
Sample on glass slide, methylene blue stain added, cover slip is put on at 90 degrees
What are the different types of electron microscope?
Transmisson em, scanning em
Disadvantages of an electron microscope?
Expensive, complex sample prep, vacuum required, dead
Advantages of an electron microscope?
High magnification and resolution, more can be seen
Maximum magnification and resolution?
Magnification- X500,00 Resolution- tem, 0.5nm. Sem, 3-1-nm
What is the function of a plasma membrane?
Regulate transport, selectively permeable
Function of the cytoplasm?
Cell shape, store chemicals needed for metabolic reactions
Function of the nucelus?
Controls activity, genetic material chromatin and proteins
Function of the nucleolus?
Synthesis RNA and ribosomes
Function of the RER?
Site of protein synthesis, transportation
Function of the SER?
Synthesis and storage of lipids and carbohydrates
Function of the golgi body?
Recieves proteins and modifies them
Function of the vesicles?
Transportation
Function of lysosomes?
Breaks down waste material
Function of ribosomes?
Protein synthesis
Function of mitochondria?
Aerobic respiration
Function of centrosomes?
Form spindle fibres during cell division
What is the process of protein synthesis?
Instructions are transcribed from DNA to mRNA, mRNA leave the nucleus via pores, Vesicles transport to golgi body, modification, fusion with the cell surface membrane
Are pro or Euk small or big?
Pro- small euk- big
What are pro made up of?
Peptidoglycogen
What are euk made up of?
Cellulose
Are pro or Euk made up of membrane bound organelles?
Euk
Dna structure of pro?
Free floating
Dna structure of euk?
Held in the nucelus
Size of ribosomes in euk?
80s
Size of ribosomes in pro?
70s
are chloroplasts present in pro or euk?
euk
Are mitrochondria present in pro or euk?
Euk
are golgi, lysosomes, er present in pro or euk?
euk
are slime capsule of flagella present in pro or euk?
pro
is the cyptoplasm present in pro or euk?
both
Describe some functions of neutrophils
Initial response to infection, phagocytosis, medidate inflamation
Describe the functions of monocytes
Fighting infection, immune response regulation, wound healing
Describe the functions of eosinophils
Protection against pathogens, allergic reactions, cell response
Describe the functions of lymphocytes
Prodction of antibodies, immune response regulation, memory cells
Describe the functions of basophils
Immune response, allergic reaction, blood flow
Mast cells function?
alert system, allergic reaction, immune response
Describe the first step in the process of phagocytosis
Detection- forgein antibodies bind to the receptors on the cell surface of phagocytes
Describe the second step in the process of phagocytosis
Engulfing- phagocyte moves towards the pathogen. Cytoplasm surronds the pathogen and is engulfed. Sealed into a phagosome inside the cytoplasm
Describe the third step in the process of phagocytosis
Lysosomes- contain proteolytic enzymes. Fuse with phagosome and release these enzymes, this breaks down the pathogen
Describe how antibodies work
Produced by lympocytes, bind to antigens which contain pathogen and attack them
Virus- what karyotic?
Akayrotic
Bacteria- what kayrotic?
Porkayrotic
Fungi- what kayrotic?
Eukayrotic
Protoctista- what kayrotic?
Eukayrotic
Disease caused by viruses?
Common cold, flu, covid, hiv
Disease caused by bacteria?
Salmonella, tb, ecoli, strep
Disease caused by fungi?
Athletes foot
Disease caused by protoctista?
Malaria
What are some methods of transmission?
Direct or social contact, airbone, contaminated food, bodily fluids
Define the term endemic
When an infectious disease is always present in an area
Define the term epidemic
A sudden outbreak of disease
Define the term pandemic
If a disease spreads across a large area
How can transmission be reduced?
Travel restrictions, PPE, immunisation
What is antibiotic resistance?
A drug doesnt have an affect on killing bacteria
What causes antibiotic resistance?
Defnese, poor infection control, overuse, agriculture
What are the problems with antibiotic resistance?
Difficulty treating, allergies to alternatives
What are the problems with making new antibiotics?
Expensive, time consuming, high demand, waste, guidelines
How can antibiotic resistance be reduced?
New antibiotics, only perscribing when needed, improved sanitsation, educating doctors
Gram positive bacteria
Purple stain, peptidoglycon, periplasmic space, plasma membrane, cytoplasm, thick cell wall, penicillin kills
Gram negative bacteria
Pink/red stain, lipopolysachoride outer membrane, periplasmic space, peptidoglycon, periplasmic space, plasma membrane
What is vaccination?
Injecting a specific type of dead or weakened pathogen into someone.
How does vaccination work?
Injection stimulates immune response, memory cell production occurs
What is active immunity?
The body makes its own antibodies
What is passive immunity?
Given ready made antibodies naturally
Vaccination progamme in the uk?
Age appropriate, cares for the vunnerable
What makes a vaccination programme sucessful?
If it creates herd immunity