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MT6317 CLIN CHEM 1 > U4 LAB: BUN > Flashcards

U4 LAB: BUN Flashcards

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1
Q

Nitrogen-containing compounds measured after removal of proteins in specimen

A

Non-Protein Nitrogen

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2
Q

NPNs are measured by converting nitrogen to?

A

ammonia

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3
Q

Subsequent reaction with what reagent forms a yellow product?

A

Nessler’s reagent (K2[HGI4])

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4
Q

Specimen for NPNs

A

Urine and Plasma

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5
Q

Urea is also known as?

A

Carbamide

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6
Q

Highest concentration in blood, major excretory product of protein metabolism

A

Urea

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7
Q

Chemical Formula of Urea

A

CO(NH2)2

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8
Q

Clinical Applications

A
  • Evaluation of renal function
  • Assess hydration status
  • Determination of nitrogen balance
  • Aid in diagnosis of renal disease
  • Verify adequacy of dialysis
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9
Q

More appropriate term for quantification of Urea

A

Blood Urea Nitrogen (BUN)

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10
Q

Conversion factor

BUN to Urea

A

2.14

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11
Q

Conversion factor

BUN Conventional to SI

A

0.357

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12
Q

Most frequently used method

A

Enzymatic (Urease) Method

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13
Q

This enzyme catalyze hydrolysis of urea and the liberated ammonium ion is quantified.

A

Urease

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14
Q

Most common assay, best as kinetic measurement

A

Glutamate dehydrogenase (GLDH)-coupled enzymatic reaction

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15
Q

GLDH

Measures rate of disappearance of reduced ______ at what wavelength?

A

Nicotinamide adenine dinucloetide, 340 nm

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16
Q

This is used in automated systems, multilayer film reagents and dry reagent strips

A

Indicator dye

17
Q

Indicator dye measures color change due to?

A

change in pH

18
Q

This is a specific and rapid test which uses an electrode

A

Conductometric

19
Q

Conductometric

The electrode is used to measure?

A

rate of increase in conductivity procued by ammonium ions

20
Q

Reference method

A

Isotope Dilution Mass Spectrometry (IDMS)

21
Q

IDMS

Used for quantification

A

isotopically-labeled compound

22
Q

IDMS

Detects what following ionization?

A

characteristic fragments

23
Q

Samples for Urea

A

Serum, Plasma, Urine

24
Q

Urine is stable up to 4 days if stored in?

A

2-8C temp

25
Q

Reagent 1

A

R1 Tris buffer (pH 7.6)
- ADP
- a ketoglutarate
- urease
- GLDH
- sodium azide

26
Q

Reagent 2

A
  • NADH
  • sodium azide
27
Q

Standard Urea:

A

50mg/dL or 8.33 mmol/L

28
Q

Spectrophotometer must be at what wavelength?

A

340nm

29
Q

Incubation time for A1

A

27 seconds

30
Q

Incubation time for A2

A

90 seconds

31
Q

Temperature

A

37C

32
Q

Conversion Factor

BUN to Urea

A

2.14

33
Q

Reference Value

Plasma or Serum

A

6-20mg/dL

34
Q

Reference Value

24 hr urine

A

12-20 g/day

MT6317 CLIN CHEM 1 (11 decks)

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