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[3Y1S] MT 6317 > U2.3 NPN : Uric Acid [L] > Flashcards

U2.3 NPN : Uric Acid [L] Flashcards

1
Q

One of the non-protein nitrogenous compounds

A

Blood Uric Acid

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2
Q

Byproduct of purine nucleic acid catabolism

A

Blood Uric Acid

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3
Q

where is BUA produced

A

liver

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4
Q

where is BUA filtered

A

glomerulus

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5
Q

% of BUA is reabsorbed in the proximal tubules

A

98-100%

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6
Q

Main form of BUA in Plasma

A

monosodium urate

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7
Q

T/F Clinical application of BUA include to confirm diagnosis and monitor treatment of gout

A

T

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8
Q

T/F Clinical application of BUA include prevent uric acid nephropathy during chemotherapeutic treatment

A

T

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9
Q

T/F Clinical application of BUA include assess inherited disorders of pyrimidine metabolism

A

F; purine

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10
Q

T/F Clinical application of BUA include to detect liver dysfunction

A

F; kidney

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11
Q

T/F Clinical application of BUA include assist in the diagnosis of renal calculi

A

T

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12
Q

BUA Determination

Uric Acid > Allantoin uses what enzyme

A

Uricase

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13
Q

BUA Determination : Chemical Method

based on reduction of phosphotungstic acid in alkaline solution to tungsten blue

A

Caraway Method

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14
Q

BUA Determination : Chemical Method

non-specific and requires protein removal

A

Caraway Method

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15
Q

BUA Determination : Chemical Method

In a carbonate solution (Na2CO3/OH-):
uric acid + H3PW12O40 + O2 →

A

allantoin + tungsten blue + CO2

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16
Q

BUA Determination

Uses uricase enzyme to catalyze oxidation of uric acid to allantoin

A

Enzymatic Methods

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17
Q

BUA Determination

More specific and more commonly used

A

Enzymatic Methods

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18
Q

BUA Determination : Enzymatic Methods

measures the hydrogen peroxide produced as uric acid is converted to allantoin

A

Coupled enzymatic reaction

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19
Q

BUA Determination : Enzymatic Methods

color produced is directly proportional to uric acid concentration

A

Coupled enzymatic reaction

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20
Q

BUA Determination : Enzymatic Methods

Coupled enzymatic reaction sources of error

A

reducing agents (bilirubin and ascorbic acid)

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21
Q

BUA Determination : Enzymatic Methods

In coupled enzymatic reactions, hydrogen peroxide produced as uric acid is converted to _____

A

allantoin

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22
Q

BUA Determination : Enzymatic Methods

measures differential absorbance before and after incubation with uricase at 293 nm

A

Spectrophotometric assay

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23
Q

BUA Determination : Enzymatic Methods

Spectrophotometric assay sources of error

A

presence of protein, hemoglobin and xanthine

24
Q

BUA Determination : Enzymatic Methods

difference is directly proportional to uric acid concentration

A

Spectrophotometric assay

25
Q

BUA Determination : Other Methods

typically uses UV detection

A

High-performance liquid chromatography (HPLC)

26
Q

BUA Determination : Other Methods

proposed candidate reference method

A

Isotope Dilution Mass Spectrometry (IDMS)

27
Q

BUA Determination : Other Methods

detects characteristic fragments following ionization

A

Isotope Dilution Mass Spectrometry (IDMS)

28
Q

BUA Determination : Other Methods

quantifies uric acid using isotopically labeled compound

A

Isotope Dilution Mass Spectrometry (IDMS)

29
Q

Specimen Considerations in BUA Determination

Specimens

A

heparinized plasma, serum or urine

30
Q

T/F In BUA Determination, fasting is not required

A

T

31
Q

Hemolytic samples

Falsely Increase/Decrease

A

Falsely decrease

32
Q

Icteric samples

Falsely Increase/Decrease

A

Falsely decrease

33
Q

Lipemic Samples

Falsely Increase/Decrease

A

Falsely increase; background interference

34
Q

Additives that should not be used for BUA determination

A

EDTA and Fluoride additives

35
Q

Assay requirements

Wavelength
Optical path
Temperature

A

Wavelength: 546 nm
Optical path: 1 cm
Temperature: 37 ͦ C

36
Q

Assay Requirements

should be read against _____

A

reagent blank

37
Q

Concentration of Standard

Urine

A

88 mg/dL

38
Q

Concentration of Standard

Serum or plasma

A

6 mg/dL

39
Q

Reference Range Plasma or Serum

Male Adult

A

3.5-7.2

40
Q

Reference Range Plasma or Serum

Female Adult

A

2.6-6.0 mg/dL

41
Q

Reference Range Plasma or Serum

Child

A

2.0-5.5 mg/dL

42
Q

Reference Range

Urine (24h)

A

250-750 mg/day

43
Q

Clinical Significance

caused by gout, lactic acidosis, chronic renal disease, drugs & poisons

A

Hyperuricemia

44
Q

Clinical Signifance

caused by liver disease, defective tubular reabsorption, chemotherapy & overtreatment w allopurinol

A

Hypouricemia

45
Q

Clinical Significance

Defective tubular reabsorption

A

Fanconi syndrome

46
Q

Sources of Error (falsely increase or falsely decrease)

Bilirubin

A

falsely increase / false positive

47
Q

Sources of Error (falsely increase or falsely decrease)

Vitamin C

A

falsely increase / falsely positive

48
Q

Sources of Error (falsely increase or falsely decrease)

Presence of protein

A

falsely increase

49
Q

Sources of Error (falsely increase or falsely decrease)

Hemoglobin

A

falsely decrease

50
Q

Sources of Error (falsely increase or falsely decrease)

Xanthine

A

falsely decrease

51
Q

Color of quinoneimine dye

A

red-violet

52
Q

enzyme that hastens oxidation of uric axid to allantoin

A

Uricase

53
Q

In the presence of uricase, uric acid is converted to ___ and _____

A

allantoin and hydrogen peroxide

54
Q

The formed hydrogen peroxide reacts under the catalysis of what enzyme?

A

Peroxidase

55
Q

Hydrogen peroxide + 4-AAP + EHSPT >

A

quinoneimine + HCl + 4H2

[3Y1S] MT 6317 (4 decks)

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