U2.2 Glycosylated Hemoglobin : Nycocard [L]

Question 1

Formation of a hgb compound produced when glucose reacts with the amino group of hemoglobin

Answer 1

Glycosylated Hemoglobin

Question 1

Glycosylated Hgb

The glucose molecule attaches non enzymatically to the hgb molecule to form a ________

Answer 1

Ketoamine

Question 2

T/F The rate of formation is directly proportional to the plasma glucose concentration

Answer 2

T

Question 3

Glycosylated Hgb

T/F Reflects the average blood glucose level over the previous 2 to 3 months

Answer 3

T

Question 4

More reliable method of monitoring long-term diabetes control than random plasma glucose

Answer 4

HbA1c

Question 5

HbA1c

Current ADA guidelines recommend that an HbA1c test be performed at least ___________ with patients who are meeting treatment goals and who have stable glycemic control

Answer 5

2x a year

Question 6

HbA1c

For patients whose therapy has changed or who are not meeting glycemic goals, a __________ is recommended

Answer 6

a quarterly HbA1c test

Question 7

HbA1c Specimen Collection and Storage

Specimen requirement

Answer 7

EDTA Whole blood sample

Question 8

HbA1c Specimen Collection and Storage

T/F Patients need not be in fasting state

Answer 8

T

Question 9

HbA1c Specimen Collection and Storage

Whole blood may be stored at ___°C for up to 1 week

Answer 9

4°C

Question 10

Measuring Principle

a reflectometer

Answer 10

The NycoCard READER II

Question 11

Measuring Principle

measures light reflected from the coloured surface of the NycoCard tests in three parts of the visible spectrum and calculates the test concentration

Answer 11

The NycoCard READER II

Question 12

Measuring Principle

T/F In the nycocard reader, the reflected light from the test sample is measured relative to a white sample

Answer 12

T

Question 13

The NycoCard HbAic kit materials

Answer 13

• 24 tubes prefilled with reagent
• 1 vial of washing solution
• 24 test devices

Question 14

Assay Test Procedure (4 steps)

Answer 14

1. Dilute and lyse sample
2. Apply sample
3. Apply wash solution
4. Read the result

Question 15

3 Steps to operate the NycoCard Reader

Answer 15

1. Adjusting
2. White calibration
3. Measure Test

Question 16

Nycocard Reader Results should be read within (n) minutes

Answer 16

5 minutes

Question 17

NycoCardTM HbA1c Test Principle

Answer 17

boronic acid affinity test principle

Question 18

affinity of boronic acid to the cis-diol configuration of glycated hemoglobin

Answer 18

boronic acid affinity test principle

Question 19

measures the color intensity of reflected light

Answer 19

NycoCard HbA1c Test Principle

Question 20

HbA1c Reference Range

Answer 20

Normal : <5.7%
Impaired : 5.7% - 6.4%
DM : >6.4%

Question 21

Significance of HbA1c in Diabetic Patients

T/F The incidence of retinopathy increases in patients with a HbA1c level between 6.0 to 7.0%

Answer 21

T, HbA1c level between 6.0 to 7.0%

Question 22

Significance of HbA1c in Diabetic Patients

T/F Fewer chances for retinopathy when the HbA1c level is <7.5%

Answer 22

F, should be <6.5%

Question 23

Significance of HbA1c in Diabetic Patients

T/F HbA1c level in diabetic patients recommended <7.0%

Answer 23

T

Question 24

Significance of HbA1c in Diabetic Patients T/F HbA1c should be checked at least once a year

Answer 24

F, twice a year

Question 25

Significance of HbA1c in Diabetic Patients T/F This is suggested that HbA1c level above 6.5% favor diabetes mellitus

Answer 25

T

Question 26

Limitations to interpret the HbA1c results from patients with conditions such as (6)

Answer 26

1. Hemolytic anemia or other hemolytic diseases 2. Homozygous sickle cell trait 3. Pregnancy 4. Blood loss 5. Polycythemia 6. Iron deficiency

Question 27

Limitations of HbA1c T/F Diluted samples cannot be used

Answer 27

T

Question 28

Limitations of HbA1c T/F Coagulated or hemolyzed samples can be used

Answer 28

F, cannot be used

Question 29

Limitations of HbA1c If the sample has a hemoglobin value below _____ g/dL or above _____ g/dL

Answer 29

below 6.0 g/dL or above 20.0 g/dL