U2.2 Glycosylated Hemoglobin : Nycocard [L] Flashcards
Formation of a hgb compound produced when glucose reacts with the amino group of hemoglobin
Glycosylated Hemoglobin
Glycosylated Hgb
The glucose molecule attaches non enzymatically to the hgb molecule to form a ________
Ketoamine
T/F The rate of formation is directly proportional to the plasma glucose concentration
T
Glycosylated Hgb
T/F Reflects the average blood glucose level over the previous 2 to 3 months
T
More reliable method of monitoring long-term diabetes control than random plasma glucose
HbA1c
HbA1c
Current ADA guidelines recommend that an HbA1c test be performed at least ___________ with patients who are meeting treatment goals and who have stable glycemic control
2x a year
HbA1c
For patients whose therapy has changed or who are not meeting glycemic goals, a __________ is recommended
a quarterly HbA1c test
HbA1c Specimen Collection and Storage
Specimen requirement
EDTA Whole blood sample
HbA1c Specimen Collection and Storage
T/F Patients need not be in fasting state
T
HbA1c Specimen Collection and Storage
Whole blood may be stored at ___°C for up to 1 week
4°C
Measuring Principle
a reflectometer
The NycoCard READER II
Measuring Principle
measures light reflected from the coloured surface of the NycoCard tests in three parts of the visible spectrum and calculates the test concentration
The NycoCard READER II
Measuring Principle
T/F In the nycocard reader, the reflected light from the test sample is measured relative to a white sample
T
The NycoCard HbAic kit materials
- 24 tubes prefilled with reagent
- 1 vial of washing solution
- 24 test devices
Assay Test Procedure (4 steps)
- Dilute and lyse sample
- Apply sample
- Apply wash solution
- Read the result
