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[3Y1S] MT 6320 > U2.2.2 Gr (+) Cocci : Laboratory Diagnosis > Flashcards

U2.2.2 Gr (+) Cocci : Laboratory Diagnosis Flashcards

1
Q

Samples preferred

A

Aspirates are preferred

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2
Q

Swabs submitted

A

Swabs are submitted, it must be at least 2

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3
Q

Gram Stained Smears are used for ____

A

Direct Detection

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4
Q

Culture Media for Isolation and Identification

A

BAP, CAP, Broth, Selective Media, Selective & Differential Media

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5
Q

Broth used in Culture Media

A

Thioglycollate, BHI, Blood-Broth

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6
Q

Selective Media used in Culture Media

A

Columbia Colistin Nalidixic Agar, PEA

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7
Q

Selective and Differential Media used in Culture Media

A

MSA, CHROMagar Staph aureus

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8
Q

Colonial Characteristics

BAP

A

Round, Smooth, White or Yellow pigmented, creamy colonies

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9
Q

Hemolysis seen in S. aureus colonies

A

Beta-hemolvsis

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10
Q

Staph spp.
Colonies are small-medium sized, gray colonies; non-hemolytic

A

S. epidermidis

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11
Q

Staph spp.
medium sized colonies, yellow pigmented colonies

A

S. saprophyticus

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12
Q

Staph spp.
medium sized colonies; Beta-hemolytic

A

S. lugdunensis

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13
Q

Staph spp.
pinhead colonies (greenish discoloration) on CAP

A

S. aureus

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14
Q

detection of enzyme catalase

A

Catalase Test

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15
Q

Catalase Test
converts H2O2 to water and O2

A

Catalase

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16
Q

Catalase Test
Reagent

A

3% H2O2

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17
Q

Catalase Test
(+) Result Test

A

Bubbling/Effervescence

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18
Q

Important Test to differentiate
Staphylococcus and Micrococcus from Streptococcus

A

Catalase Test

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19
Q

Oxidase reagent reacts with cytochrome C in the cytochrome oxidase

A

Modified Oxidase Test

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20
Q

Uses Rgt: 6% tetramethyl phenylenediamine HCI
in dimethyl sulfoxide (DMSO)

A

Modified Oxidase Test

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21
Q

Modified Oxidase Test
(+) Result

A

Dark Blue

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22
Q

Differentiates Staphylococcus from
Micrococcus

A

Modified Oxidase Test

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23
Q

detection of staphylocoagulase (coagulase)

A

Coagulase Test

24
Q

uses Rabbit Plasma

A

Coagulase Test

25
Q

Two Types of Coagulase Test

A
  1. Slide Coagulase (Screening)
  2. Tube Coagulase (Confirmatory)
26
Q

to differentiate organisms based on the ability to oxidize or
ferment sugars

A

Oxidation Fermentation Test

27
Q

Oxidation Fermentation Test
(+) Color of Fermenters

A

Yellow

28
Q

Oxidation Fermentation Test
(+) Color of Oxidizers

A

Red + Yellow

29
Q

Oxidation Fermentation Test
(+) Color of Non-utilizer

A

Red

30
Q

detection of DNAse activity

A

DNAse Test

31
Q

Reagent: Use of Toluidine Blue or methyl Green

A

DNAse Test

32
Q

DNAse Test

(+) Result

A

Hydrolysis of surrounding medium (clear zone)

33
Q

formation of acetoin from glucose (Butylene Glycol Pathway)

A

Voges Proskauer Test

34
Q

Differentiates Coagulase (+) Staphylococcus

A

Voges Proskauer Test

35
Q

Voges Proskauer Test
Positive Result

A

Red coloration in MRVP Broth

36
Q

Differentiates Coagulase (+) Staphylococcus

A

PYR Test (Pyrrolidonyl Arilamadase) Test

37
Q

PYR Test (Pyrrolidonyl Arilamadase) Test
(+) Result

A

Pink or Cherry-Red coloration

38
Q

Useful Test to differentiate CONS

A

Novobiocin (5ug)
Susceptibility

39
Q

T/F Staphylococcus saprophyticus is resistant to novobiocin, indicated by the lack of a zone of inhibition around the disk.

A

T

40
Q

Helpful for CONS

A

Susceptibility to Polymyxin B (300 units)

41
Q

Most useful test for confirming S. lugdunensis

A

Ornithine Decarboxylase Test

42
Q

Ornithine Decarboxylase Test
(+) Result

A

Violet Coloration on ODC tube

43
Q

used to differentiate S. aureus from CONS

A

Commercially Available Rapid Agglutination Kits

44
Q

useful for ID MRSA

A

Commercially Available Rapid Agglutination Kits

45
Q
  • more specific and sensitive from traditional methods
  • use of plasma coated latex beads
A

Commercially Available Rapid Agglutination Kits

46
Q

Detection of Resistance
Detection of resistance to macrolides such as clindamycin

A

Macrolide Resistance

47
Q

Detection of Resistance
Method used in Macrolide Resistance

A

D-Zone Test

48
Q

Detection of Resistance
Place Erythromycin and Clindamycin Disk close together

A

D-zone Test

49
Q

Detection of Resistance
Detection of mecA gene - gold standard

A

MRSA

50
Q

Detection of Resistance
Recommended Screening for MRSA

A

Cefoxitin Test

51
Q

Detection of Resistance

MIC of VRSA

A

> 16 ug/mL

51
Q

Detection of Resistance
Use of Vancomycin Agar Plate (spot inoculation)

A

VRSA

52
Q

Detection of Resistance
T/F Healthcare Associated MRSA include sepsis, infection of surgical wound sites, pneumonia

A

T

52
Q

Detection of Resistance

MIC of MRSA

A

≥ 4ug/mL

53
Q

Detection of Resistance

Treatment of VRSA

A

Linezolid

[3Y1S] MT 6320 (8 decks)

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