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MT6318 HEMA 1 > U2 LAB: PBS PREPARATION > Flashcards

U2 LAB: PBS PREPARATION Flashcards

1
Q

Evaluation of peripheral blood film is an intergal part of?

A

CBC or hemogram

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2
Q

PBS is required for?

A

enumeration and checking of cellular elements

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3
Q

Microscopic examination aids in providing info regarding?

A

formed elements in blood

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4
Q

Reliability of information obtained depends on?

A

well-made and well-stained films

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5
Q

T/F: Smears must be prepared immediately as possible.

A

True

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6
Q

Blood sample

A

Anticoagulated EDTA blood

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7
Q

Stain used

A

Wright or Wright-Giemsa stain

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8
Q

Systematic approach:

A
  1. 10x
  2. 40x
  3. OIO
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9
Q

Materials needd:

A
  • Glass slides, coverslips
  • Pasteur pipette
  • Anticoagulated blood
  • Tube holder
  • Pencil for labeling
  • PPEs
  • Canister with yellow bag
  • Sharps container
  • Oil cloth
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10
Q

Method 1

A

Two-Slide or Wedge method

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11
Q

Simplest and most common method for smear prep

A

Two-Slide or Wedge method

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12
Q

Procedures for Method 1

Size of drop ng blood

A

2-3mm in diameter

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13
Q

Procedures for Method 1

Drop of blood should be placed where?

A

1cm from the end

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14
Q

Procedures for Method 1

Fingers used to hold spreader slide

A

thumb and forefinger

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15
Q

Procedures for Method 1

Angle of spreader slide

A

30-45 degrees

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16
Q

Features of a Properly Made Blood Smear

The film must cover?

A

2/3 to 3/4 length

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17
Q

Features of a Properly Made Blood Smear

The film is what shape?

A

finger shaped (rounded at feather edge)

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18
Q

Features of a Properly Made Blood Smear

What edges should be visible?

A

lateral edges

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19
Q

Features of a Properly Made Blood Smear

The film is smooth without irregularities, like?

A

holes or streaks

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20
Q

Features of a Properly Made Blood Smear

When slide is held up to light, feathery edge should have a?

A

rainbow appearance

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21
Q

Portion observed in feathery edge

A

Zone of morphology

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22
Q

Unacceptable Peripheral Blood Smears and its Causes

A
  • chipped or rough edge
  • hesitation in forward motion
  • pushed too quickly
  • drop of blood was too small
  • not allowed to spread across the width of slide
  • dirt or grease
  • uneven pressure
  • time delay
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23
Q

T/F: Hematocrit levels can be attributed to smears.

A

True

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24
Q

Low hematocrit levels

A

Too thin

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25
Q

High hematocrit levels

A

Too thick

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26
Q

Reminders once you have created the smear

A good film includes a?

A

thick and thin portion, with gradual transition

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27
Q

Moving the pusher slide forward too slowly accentuates?

A

poor leukocyte distribution (pushing larger cells at very end of film)

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28
Q

The faster the film is air dried,

A

the better is the spreading

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29
Q

Slow drying causes?

A

formation of artifactual cell

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30
Q

Slide may be labeled on the frosted end or directly on the thicker end using?

A

lead pencil

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31
Q

The thickness of the film can be adjusted by?

A

changing the angle or speed of spreading

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32
Q

This can cause erroneous result in preparing the smear

A

high lipid content

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33
Q

Older technique that is used for making bone marrow aspirate smears.

A

Two-Coverslip or Two-Coverglass method

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34
Q

Method 2

A

Two-Coverslip or Two-Coverglass method

35
Q

Recommended coverslips for Method 2

A

No. 1 or 1 1/2 cover glasses 22mm

36
Q

Quality coverslip smear will appear similar to a?

A

thumb print

37
Q

Making smears on coverslips require?

A

manual dexterity

38
Q

Advantage of Method 2

A
  • excellent leukocyte distribution
  • minimal amount of bone marrow is needed but many smears are made
39
Q

Procedures for Method 2

Hold one coverglass on adjacent corners with?

A

Thumb and index finger

40
Q

Procedures for Method 2

Blood must form a?

A

16-sided figure (letter B)

41
Q

Procedures for Method 2

Before blood spreads, seperate coverglass by?

A

rapid, even, horizontal and lateral pull

42
Q

Method 3

A

Spinner Method

43
Q

This method would create a uniform blood film.

A

Spinner Method

44
Q

Easy handling of wedge slide and uniform distribution of cells can be made with special types of centrifuges known as?

A

spinners

45
Q

Spinner slide produces film in which?

A

all cells are separated (monolayer) and randomly distributed

46
Q

Smears prepared using Method 3 have what that can be easily identified at any spot in the film?

A

white blood cells

47
Q

On wedge smear, disproportions of ____ occur at tip of feather edge

A

monocytes

48
Q

____ are seen just in from the feather edge

A

Neutrophils

49
Q

___________ may occur at lateral edges of the films

A

Neutrophils and Monocytes

50
Q

This is an automated slide making and staining system.

A

Sysmex SP-1000i

51
Q

Automated Smear Preparation is dependent on?

A

hematocrit values

52
Q

Film for automated smear prep is produced every ____ and with what?

A

30 seconds, printed label

53
Q

Printed label for Automated Smear Prep contains?

A
  • Patient name
  • Number
  • Date of preparation
54
Q

This prepares slides automatically based on orders received from the LIS

A

DxH Slidemaker Stainer II Cellular Analysis System by Beckman Coulter

55
Q

DxH Slidemaker Stainer II Cellular Analysis System by Beckman Coulter

This creates and stains smears from?

A
  • capped whole blood tubes
  • open-top tubes
  • pediatric whole blood tubes
56
Q

DxH Slidemaker Stainer II Cellular Analysis System by Beckman Coulter

How many slides can it create from a single 90uL sample?

A

4 slides

57
Q

DxH Slidemaker Stainer II Cellular Analysis System by Beckman Coulter

How many slides can it create from a single sample presentation?

A

12 slides

58
Q

DxH Slidemaker Stainer II Cellular Analysis System by Beckman Coulter

This creates multiple stain smear slides according to?

A

any parameter results

59
Q

Wright stain is composed of?

A
  • Eosin
  • Complex mixture of (Thiazines) including methylene blue, azure blue
60
Q

This staining technique is certified by the Biological Stain Commission and is commercially available as solution or powder.

A

Staining Jar or Dip Method

61
Q

T/F: Polychrome stains contain Eosin and Methylene blue.

A

True

62
Q

Heparin causes?

A

blue background

63
Q

Oxalate and Fluoride causes?

A

distortion of cell morphology

64
Q

Reagents in staining dip or jar method is found in?

A

coplin jars

65
Q

Solution 1

A

Methanol, 30 seconds

66
Q

This will serve as fixative.

A

Methanol

67
Q

Solution 2

A

Eosin, 6 seconds

68
Q

Free eosin is acidic and stains basic components such as?

A

Hemoglobin or eosinophilic granules (red)

69
Q

Solution 3

A

Methylene blue, 4 seconds

70
Q

Free methylene blue is basic and stains acidic cellular components such as?

A

RNA (blue)

71
Q

Solution 4

A

Buffer solution/aged distilled water, 45 seconds

72
Q

Buffer solution

A

0.05M sodium phosphate (pH 6.4)

73
Q

Aged distilled water

A

Placed in glass bottle for at least 24 hours; pH 6.4 to 6.8)

74
Q

Slide must dry in a?

A

tilted position

75
Q

Color of RBCs

A

pink to salmon

76
Q

Color of Nuclei

A

dark blue to purple

77
Q

Cytoplasmic granules of Neutrophils

A

lavender to lilac

78
Q

Cytoplasmic granules of Basophils

A

dark blue to black

79
Q

Cytoplasmic granules of Eosinophils

A

red to orange

80
Q

Area between the cells should be?

A

colorless, clean, free of precipitated stain

81
Q

Slides must be prepared within how many hours following blood collection?

A

2 to 3 hours

82
Q

Oxidation on staining causes development of?

A

Formic acid

83
Q

Other romanowsky-type stains:

A
  • Giemsa
  • Leishman’s
  • Jenner’s
  • May-grunwald
  • MacNeal’s

MT6318 HEMA 1 (6 decks)

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