U1 LAB: LAB SAFETY Flashcards
Most important procedure in healthcare to prevent spread of infection according to WHO
Hand Hygiene
Hand hygiene prevents what kind of infections?
Nosocomial
This refers to agent transfer within healthcare premises.
Nosocomial infections
First maintenance of hand hygiene
Proper handwashing
T/F: Proper handwashing requires a specific procedure in a workplace.
True
Essentials of handwashing
- Water
- Soap
- Manner on how to do it
Most important aspect of handwashing
Friction Scrubbing
How many seconds must be allotted for friction scrubbing?
20 seconds
How many seconds/minutes must be allotted for surgical handwashing?
2 minutes
Surgical handwashing must be up until the?
arms
When should you wash your hands?
- entering, leaving the laboratory
- touching body fluids or chemicals
- changing PPEs
- in between patient contact
Second maintenance of hand washing
Hand sanitizers
Examples of hand sanitizers
70% isopropyl alcohol or ethyl alcohol
Disinfectant is used for?
surfaces
Sanitizer is used for?
living things
T/F: Alcohol is both sanitizer and disinfectant.
True
Proper order of Donning
GoMaGlo (Gown, Mask, Gloves)
Proper order of Doffing
GloGoMa (Gloves, Gown, Mask)
Pipette used in Hematology
Thoma pipette
This reagent is used to lyse RBCs.
Acetic acid
This refers to the historic way, and is prone to exposure to chemical and biohazard.
Mouth pipetting
Sharps disposal is done thru a?
Sharps container
This is an enclosed space for the sharps, and serves as both biohazard and physical hazard.
Sharps container
Characteristics of the Sharps Container
- Tight fitting lid
- Puncture proof
- Leak free
- Rigid
- Has biohazard symbol
Color of the sharps container
Red and yellow
Yellow color stands for?
Infectious substances, biohazard
Red color stands for?
Physical hazard
Sharps have this mechanism that prevent the spread of hazard.
Engineering controls
Most common injury in the lab
Needlestick injury
Dilution of bleach for decontamination
1:10
Water allows better what of the cell wall
Penetrate
Identify for what spill the procedure is:
- Wipe with absorbant pad (Tissue/paper towel)
- Discard to yellow bag
- Decontaminate area (with diluted bleach)
- Wash hands
Small spills (Wet drops)
Identify for what spill the procedure is:
- Moisten area with disinfectant (with diluted bleach)
- Absorb with tissue/towel
- Discard in yellow bag
- Disinfect area (with diluted bleach)
Small spills (Dried Drops)
Identify for what spill the procedure is:
- Use special clay/powder (chlorine based)
- Scoop/sweep, discard then disinfect
Large spill
Identify for what spill the procedure is:
- Use heavy duty gloves
- Scoop/sweep area
- Discard to sharps container then disinfect
Spills with broken glass
Solution 1
Fixative: Methanol
Solution 2
Eosin (Acidic dye)
Solution 3
Methylene blue
Solution 4
Aged distilled water/buffer
Chemicals must be properly?
labeled
This refers to the ability to transform to liquid then gas form.
Volatile
MSDS stands for?
Material Safety Data Sheet
MSDS contains?
- Name
- Manufacturer
- Hazardous ingredients
- Physical and chemical characteristics
- Documentation
- Precautionary measures
- Control measures
Fire Extinguisher A is for?
papers, wood, cloth, plastic (highly combustible)
Fire Extinguisher B is for?
flammable liquids, gases, greases
Fire Extinguisher C is for?
electricals
This is used to extinguish reactive materials
Halons
RACE stands for?
Rescue, Alarm, Contain, Extinguish
PASS stands for?
Pull, Aim, Squeeze, Sweep
Safety Program Elements
- Written safety plan
- Training programs
- Job Safety Analysis
- Risk assessment
- Safety audits (Follow ups)
- Reporting
- Investigation of accidents
- Emergency drills
What is best object to combat electrical hazard?
Glasswares
Alternative objects to use for electrical hazards
Wood or rubber