U2 Chapter 6: A Tour of the Cell Flashcards
Light Microscope (LM)
Visible light is passed through the specimen and then through glass lenses, the lenses refract (bend) the light in such a way that the image of the specimen is magnified as it is projected into the eye or into a camera
Magnification
The ratio of an object’s image size to its real size
Resolution
A measure of the clarity of the image; it is the minimum distance two points can be separated and still be distinguished as separate points
Organelles
The membrane-enclosed structures within eukaryotic cells
Electron Microscope (EM)
Focuses a beam of electrons through the specimen or onto its surface
Scanning Electron Microscope (SEM)
Useful for detailed study of the topography of a specimen where the electron beam scans the surface of the sample, usually coated with a thin film of gold
- the result is an image of the specimen’s surface that appears 3D
Transmission Electron Microscope (TEM)
Used to study the internal structure of cells where the TEM aims an electron beam through a very thin section of the specimen, much as a light microscope aims light through a sample on a slide
Cell Fractionation
Technique for studying cell structure and dunction which takes cells apart and separates major organelles and other subcellular structures from one another
Differential Centrifugation
The piece of equipment that is used for this task is the centrifuge, which spins test tubes holding mixtures of disrupted cells at a series of increasing speeds
Cytosol
Semifluid and jellylike substance where subcellular components are suspended
Eukaryotic Cell
Most of the DNA is in an organelles called the nucleus, which is bounded by a double membrane
Prokaryotic Cell
The DNA is concentrated in a region that is not membrane-enclosed nucleoid
Cytoplasm
Interior in all cells
- for eukaryotic cells, this term refers only to the region between the nucleus and the plasma membrane
Nucleus
Contains most of the genes in the eukaryotic cell (some genes are located in mitochondria and chloroplasts)
Nuclear Envelope
Encloses the nucleus, separating its contents from the cytoplasm
Nuclear Lamina
A netlike array of protein filaments (in animal cells, called intermediate filaments) that maintains the shape of the nucleus by mechanically supporting the nuclear envelope
Chromosomes
Structures that carry the genetic information that DNA units are organized into
Chromatin
Complex DNA and proteins making up chromosomes
Nucleolus (plural for nucleoli)
Appears through the electron microscope as a mass of densely stained granules and fibers adjoining part of the chromatin
Ribosomes
Complexes made of ribosomal RNAs and proteins, are the cellular components that carry out protein synthesis
Endomembrane System
Different membrane-bounded organelles of the eukaryotic cell, which includes the nuclear envelope, the endoplasmic reticulum, the Golgi apparatus, lysosomes, various kinds of vesicles and vacuoles, and the plasma membrane
Vesicles
Sacs made of membrane for membrane transfer segments
Endoplasmic Reticulum (ER)
Extensive network of membranes that it accounts for more than half the total membrane in many eukaryotic cells
Smooth ER
Named because its outer surface lacks ribosomes
Rough ER
Studded with ribosomes on the outer surface of the membrane and thus appears rough through the electron microscope
Transport Vesicles
Vesicles in transit from one part of the cell to another