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Hemo 2 > U 2 P 2 > Flashcards

U 2 P 2 Flashcards

1
Q

Cytochemistry reflects what?

A

Chemical composition of cells

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2
Q

What cellular constituent does MPO stain?

A

Peroxidase in primary granules

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3
Q

What AMLS are mpo negative and why?

A

M0, M5, M7

Lack of granules

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4
Q

AMLS that are MPO positive?

A

M1, 2 3 4 6

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5
Q

What component does Sudan black B stain?

A

Lipids and secondary granules

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6
Q

What AMLS will be positive for SBB

A

M1, 2, 3, 4 6

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7
Q

What component does specific esterase stain?

A

Enzyme specific esterase

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8
Q

Where in the cell is specific esterase found?

A

Primary granules

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9
Q

What line would see a weak positive for specific esterase stain?

A

Monocytic

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10
Q

Non specific esterase is used to differentiate what two cell lines?

A

Myleocytic and monocytic

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11
Q

What AMLS will react positively to non specific esterase?

A

M4, 5

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12
Q

Periodic scid shiff is used for what two AMLS?

A

M6, 7

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13
Q

What is myLeoperoxidase used to differentiate

A

AML

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14
Q

SBB is positive in?

A

Grabulocytes and monocytes

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15
Q

Specific esterase will stain positive in?

A

Neutrophils and basophils

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16
Q

Tdt helps assess what?

A

Blast in bone marrow

17
Q

Tdt stains positive for?

A

90% of ALL
T cells
B cells

18
Q

Pas stains what?

A

Glycol containing parts of a cel

Glycogen, intracellular carbohydrates

19
Q

Pas will stain negative in?

A

Normal erythrocytes and myleoblasts

20
Q

LAP stains what?

A

Bands and sets

21
Q

LAP will have diminished activity in?

A

CML

22
Q

What are some differences between leukemia’s and luekemoid reactions?

A

Leukemia
WBC over 100,000
Anemia
Blasts to neutrophils

LR
WBC under 50,000
No aemia
Metas to neutrophils

Hemo 2 (7 decks)

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