U 2 P 2

Question 1

Cytochemistry reflects what?

Answer 1

Chemical composition of cells

Question 2

What cellular constituent does MPO stain?

Answer 2

Peroxidase in primary granules

Question 3

What AMLS are mpo negative and why?

Answer 3

M0, M5, M7

Lack of granules

Question 4

AMLS that are MPO positive?

Answer 4

M1, 2 3 4 6

Question 5

What component does Sudan black B stain?

Answer 5

Lipids and secondary granules

Question 6

What AMLS will be positive for SBB

Answer 6

M1, 2, 3, 4 6

Question 7

What component does specific esterase stain?

Answer 7

Enzyme specific esterase

Question 8

Where in the cell is specific esterase found?

Answer 8

Primary granules

Question 9

What line would see a weak positive for specific esterase stain?

Answer 9

Monocytic

Question 10

Non specific esterase is used to differentiate what two cell lines?

Answer 10

Myleocytic and monocytic

Question 11

What AMLS will react positively to non specific esterase?

Answer 11

M4, 5

Question 12

Periodic scid shiff is used for what two AMLS?

Answer 12

M6, 7

Question 13

What is myLeoperoxidase used to differentiate

Answer 13

AML

Question 14

SBB is positive in?

Answer 14

Grabulocytes and monocytes

Question 15

Specific esterase will stain positive in?

Answer 15

Neutrophils and basophils

Question 16

Tdt helps assess what?

Answer 16

Blast in bone marrow

Question 17

Tdt stains positive for?

Answer 17

90% of ALL
T cells
B cells

Question 18

Pas stains what?

Answer 18

Glycol containing parts of a cel

Glycogen, intracellular carbohydrates

Question 19

Pas will stain negative in?

Answer 19

Normal erythrocytes and myleoblasts

Question 20

LAP stains what?

Answer 20

Bands and sets

Question 21

LAP will have diminished activity in?

Answer 21

CML

Question 22

What are some differences between leukemia’s and luekemoid reactions?

Answer 22

Leukemia
WBC over 100,000
Anemia
Blasts to neutrophils

LR
WBC under 50,000
No aemia
Metas to neutrophils