Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology, Mycology, Virology > Types of culture media > Flashcards

Types of culture media Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Culture

A

Are growth of microorganism on a culture medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Is a liquid, semi-solid, or solid medium utilized to observe growth patterns of microorganism as well as for transport and storage

A

Culture medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Types of culture (3)

A

Pure
Mixed
Stock

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Pure culture

A

Composed only one specie

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Mixed culture

A

Composed of more than one specie

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Composed of several species contained in a separate culture medium
Should be grown in large volume of broth
Used for academic and industrial purposes

A

Stock culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Classification of culture medium according to consistency (3)

A

-Liquid medium
-Semi-solid medium
-Solid medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Contains 0% agar
Allows growth of aerobes, anaerobes and facultative anaerobes

A

Liquid medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

3 Examples of liquid medium

A

-Brain heart infusion
-Trypticase Soy Broth
-Thioglycollate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Semi-solid medium

A

Contains 0.5-1% agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Example of semi-solid medium

A

Sulfide Indole Motility

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Solid medium

A

Contains 2-3% agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

4 Examples of solid medium

A

-Triple Sugar Iron (TSI) agar
-Mac Conkey
-Blood Agar Plate
-Chocolate Agar Plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Is a sulfated polymer containing D-galactose, D-glucuronic acid; derived from red algae

A

Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Agar will melt if the temperature reaches

A

80-90

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Agar will solidify if the temperature reaches

A

40-50

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Cooling temperature for distribution of agar into Petri dish

A

55-60

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Classification of culture medium according to composition (3)

A

-Synthetic/defined Medium
-Non-synthetic/complex medium
-Tissue Culture Medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Medium where all components are known to the user
Used for research purposes

A

Synthetic/Defined medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Non-synthetic/Complex medium

A

-Composed of some unknown substances
-Useful for isolation of bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

3 Examples of non-synthetic medium

A

-Nutrient Broth (NB)
-Trypticase Soy Broth (TSB)
-Mac Conkey agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Used for obligate intracellular bacteria (Rickettsia & Chlamydia)

A

Tissue culture medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Classification of culture medium according to how the medium is dispensed/distributed (2)

A

-Plated medium
-Tube medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Tube medium (4

A

Liquid
Butt
Buttslant
Slant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Classification of culture medium according to use (6)

A

-Simple Media/General Purpose —Media/Supportive Media
-Enrichment media
-Enriched media
-Differential media
-Selective media
Special media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Routinely used in the lab and w/o added supplement
Media that support the growth of most nonfastidious bateria

A

-Simple Media/General Purpose -Media/Supportive Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Composition of Simple Media/General Purpose Media/Supportive Media

A

Meat & soybean extracts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

2 Examples of Simple Media/General Purpose Media/Supportive Media

A

-Nutrient agar (NA) and broth (NB)
-Trypticase Soy Broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Used to propagate growth of certain group of bacteria from a mixture of organisms
Contains specific type of nutrients
Liquid-type media

A

Enrichment media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Media with added supplements necessary for the growth of fastidious organisms
Solid-type media

A

Enriched/non-selective media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Standard nonselective medium

A

Blood Agar Plate (BAP)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Is with horse blood; used for isolation of hemophilus

A

Chocolate Agar Plate (CAP)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Media that allow visualization of metabolic differences between groups of bacteria

A

Differential media

34
Q

Differentiates lactose fermenters (pink colonies) from nonlactose fermenters (colorless colonies)

A

Mac Conkey

35
Q

Differentiates hemolytic pattern & what hemolytic pattern?

A

-BAP
-Streptococci

36
Q

Hemolytic patterns (3)

A

-Alpha
-Beta
-Gamma

37
Q

Greenish zone
Partial/increased hemolysis

A

Alpha

38
Q

Clear zone
Complete lysis of RBCs around colony

A

Beta

39
Q

No hemolysis
No change in agar

A

Gamma

40
Q

Media incorporated with antibiotics, dyes or chemicals to inhibit the growth of undesired organisms while promoting the growth of desired organism

A

Selective media

41
Q

6 Examples of selective media

A

-Hektoen enteric (HE) agar
-Mac Conkey agar
-Xylose Lysine Desoxycholate (XLD) agar
-Bismuth sulfite agar (BSA)
-Mannitol salt agar
-Thayer Martin agar (TMA)

42
Q

Gentamicin Blood Agar

A

Streptococci

43
Q

Bacitracin Chocolate Agar

A

Hemophilus

44
Q

Blood Agar Plate with Ampicillin

A

Aeromonas

45
Q

Phenol Ethyl Alchohol

A

Gram-positive cocci

46
Q

Columbia agar with CNA

A

Gram-positive bacteria

47
Q

Gram-negative (GN) Broth

A

-Salmonella spp.
-Shigella spp.

48
Q

Thayer Martin Agar (TMA)

A

Neisseria gonorrheae

49
Q

Special media

A

Used to isolate bacteria with specific growth requirements

50
Q

2 Examples of special media

A

-Lowenstein Jensen (LJ) medium
-Thiosulfate Citrate Bile Salt Sucrose (TCBS) agar

51
Q

Red with black centers

A

Salmonella

52
Q

Red or clear colonies without black centers

A

Shigella

53
Q

Used as a filler for the remaining percentage of the anaerobic atmosphere

A

Nitrogen gas

54
Q

Remove residual oxygen from the chamber by combining with hydrogen to form water

A

Palladium pellets

55
Q

Used to absorb the water formed when hydrogen combines with free oxygen in the presence of the catalyst

A

Silica gel (dessicant)

56
Q

An oxygen reduction indicator; it becomes colorless in the absence of oxygen

A

Methylene blue or reazurin

57
Q

Inoculation techniques (5)

A

-Streaking
-Placement if fluid specimens or swabs into culture broth
-Stabbing the medium
-Stab and streak technique
-Pour plate technique

58
Q

Most commonly performed inoculatin technique

A

Streaking

59
Q

Streaking (5)

A

-Radial streak
-Overlap streak
-Multiple streak
-Multiple interrupted
-Interrupted

60
Q

The microorganisms present in the specimen are successively diluted out as each quadrant is streaked until finally each morphotype is present as a single colony

A

Streaking fir isolation

61
Q

This technique is applied to semi-solid medium like SIM medium
Also used to inoculate streptococci into BAP

A

Stabbing

62
Q

Used to inoculate organism into the butt slant tube medium such as the TSI agar and LIA

A

Stab and streak

63
Q

Is a technique where the organism is added to a molten agar, mixed and then allows the medium to solidify

A

Pour plate technique

64
Q

pH indicators (4)

A

Phenol red
Bromthymol blue
Neutral red
Bromcresol purple

65
Q

Bromthymol blue

A

HEA
SCA
TCBS
Malonate broth

66
Q

Phenol red

A

TSI
XLD
MSI
Christensen’s urea sugar

67
Q

Neutral red

A

Mac Conkey
SSA

68
Q

Bromcresol purple

A

LIA

69
Q

Neutral Red + Acid

A

Red

70
Q

Neutral Red + Alkaline

A

Yellow

71
Q

Phenol Red + Acid

A

Yellow

72
Q

Phenol Red + Alkaline

A

Red

73
Q

Bromthymol Blue + Acid

A

Orange to Yellow

74
Q

Bromthymol Blue + Alkaline

A

Green to blue

75
Q

Thosulfate-Citrate-Bile salt Sucrose (TCBS) inhibitor, Ph Indicator, and Differentiating factor

A

Bile salt

Bromthymol blue

Sucrose

76
Q

Salmonella Shigella (SS) Agar inhibitor, Ph Indicator, and Differentiating factor

A

Bile salt

Neutral red

Lactose, H2S

77
Q

MacConkey (MAC) Agar inhibitor, Ph Indicator, and Differentiating factor

A

Crystal violet

Neutral red

Lactose

78
Q

Hektoen Enteric Agar (HEK, HE or HEA) inhibitor, Ph Indicator, and Differentiating factor

A

Bile salt

Bromthymol blue

Lactose, sucrose, salicin, H2S

79
Q

Xylose Lysine Deoxycholate Agar (XLD) inhibitor, Ph Indicator, and Differentiating factor

A

Bile salt

Phenol red

Lactose, sucrose, xylose, H2S

80
Q

Eosin Methylene Blue Agar (EMB) inhibitor, Ph Indicator, and Differentiating factor

A

Eosin Y/ MB

Eosin Y/ MB

Lactose

81
Q

Small zone of intact RBCs surrounded by a zone of Beta hemolysis

A
82
Q

Inner zone of beta hemolysis surrounded by a zone of alpha

A

Double

Microbiology, Mycology, Virology (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions