Acid Fast Bacilli staining Flashcards
What kind of staining is an Acid Fast Stain?
It is a differential stain
What do you differentiate in acid fast stain?
- Acid-fast positive bacteria.
- Acid-fast negative bacteria.
Define Acid fast bacteria
Is a bacteria that resist de-colorization when it’s exposed to acid alcohol.
On acid fast positive, do they lose their stain when acid alcohol is applied?
No, they do not lose their stain when we add acid alcohol.
What is the only condition that could force the dye out?
If we use too much alcohol.
Ziehl Neelsen Primary stain
Carbol fuchsin
Ziehl Neelsen mordant
Heat
Ziehl Neelsen decolorizer
Acid alcohol
Ziehl Neelsen secondary stain
Methylene blue
Kinyoun primary stain
Carbol fuchsin
Kinyoun Mordant
Tergitol/Phenol
Kinyoun Decolorizer
Acid alcohol
Kinyoun Secondary stain
Methylene blue
Modified Kinyoun.Primary Stain
Carbol fuchsin
Modified Kinyoun Mordant
70% ethanol
Modified Kinyoun Decolorizer
Sulfuric acid
Modified Kinyoun Secondary stain
Methylene blue
What are some acid fast positive example?
Bacilli, and they are gram positive.
What are the specific genera of bacteria that are acid fast positive? Give some example
Genera: Mycobacterium Spegmatis
On acid fast stain, if we add the primary stain carbolfuchsin, what color will the bacteria will be?
AF+ and AF- will both be pink
On acid fast stain, if we add (or apply) steam as mordant, what color will our bacteria will be?
AF+ and AF- will be pink
On acid fast stain, if we add de-colorizer (Acid alcohol) what color will our bacteria will be?
AF+ is pink
AF- will be clear
On acid fast stain, if we add counterstain (Methylene blue), what color will our bacteria be?
AF+ will be pink
AF- will be blue
