Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology, Mycology, Virology > Acid Fast Bacilli staining > Flashcards

Acid Fast Bacilli staining Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

What kind of staining is an Acid Fast Stain?

A

It is a differential stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What do you differentiate in acid fast stain?

A
  1. Acid-fast positive bacteria.
  2. Acid-fast negative bacteria.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Define Acid fast bacteria

A

Is a bacteria that resist de-colorization when it’s exposed to acid alcohol.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

On acid fast positive, do they lose their stain when acid alcohol is applied?

A

No, they do not lose their stain when we add acid alcohol.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the only condition that could force the dye out?

A

If we use too much alcohol.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Ziehl Neelsen Primary stain

A

Carbol fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Ziehl Neelsen mordant

A

Heat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Ziehl Neelsen decolorizer

A

Acid alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Ziehl Neelsen secondary stain

A

Methylene blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Kinyoun primary stain

A

Carbol fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Kinyoun Mordant

A

Tergitol/Phenol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Kinyoun Decolorizer

A

Acid alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Kinyoun Secondary stain

A

Methylene blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Modified Kinyoun.Primary Stain

A

Carbol fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Modified Kinyoun Mordant

A

70% ethanol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Modified Kinyoun Decolorizer

A

Sulfuric acid

17
Q

Modified Kinyoun Secondary stain

A

Methylene blue

18
Q

What are some acid fast positive example?

A

Bacilli, and they are gram positive.

19
Q

What are the specific genera of bacteria that are acid fast positive? Give some example

A

Genera: Mycobacterium Spegmatis

20
Q

On acid fast stain, if we add the primary stain carbolfuchsin, what color will the bacteria will be?

A

AF+ and AF- will both be pink

21
Q

On acid fast stain, if we add (or apply) steam as mordant, what color will our bacteria will be?

A

AF+ and AF- will be pink

22
Q

On acid fast stain, if we add de-colorizer (Acid alcohol) what color will our bacteria will be?

A

AF+ is pink

AF- will be clear

23
Q

On acid fast stain, if we add counterstain (Methylene blue), what color will our bacteria be?

A

AF+ will be pink

AF- will be blue

Microbiology, Mycology, Virology (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions