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Microbiology, Mycology, Virology > Laboratory Equipment > Flashcards

Laboratory Equipment Flashcards

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1
Q

focuses the light ging into the objective lens

A

Microscope- The Condenser

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2
Q

adjusts the amount of light going into the objective lens

A

Microscope- The Iris Diaphragm

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3
Q

holds the objective lenses and rotates to change from one objective to another

A

Microscope- Nosepiece

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4
Q

moves the stage larger distances to help get the object in focus

A

Microscope- Coarse Objective Knob

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5
Q

moves the stage smaler increments to help get the object in better focus

A

Microscope- Fine Adjustment Knob

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6
Q

magnifies the object. It is located near the object.

A

Microscope- Objective Lens

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7
Q

magnifies the image of the object from the objective lens. It is located near the eye.

A

Microscope- Occular Lens

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8
Q

Steps in using the microscope

A

1) scanner power in place
2) place slide of specimen stage in place with stage clips
3) Using the course adjustment knob, raise the stage all the way up and then down slowly until you can see image of specimen
4) Readjust with fine adjustment knob

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9
Q

Describe the purpose of the oil immersion lens

A

Used when oil is added to the slide. Oil must touch the object and objective lens to that the light does not refract futher

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10
Q

Parfocal

A

Parfocal microscopes are able to move from one objective to the next and still be in focus due to the length of the objective lenses

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11
Q

The ability to distinguish between two objects that are very close together.

A

Resolution

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12
Q

What is resolving power?

A

The measurement of resolution

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13
Q

What are two ways to increase resolving power?

A
  1. By decreasing the wavelength of light (using a blue filter) 2. Increasing the numerical aperature
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14
Q

What units are used to express resolving power?

A

nm or nanometers

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15
Q

What is better, smaller or larger resolving power?

A

Smaller is better
Light microscopes have a 200nm resolving power
Electron microscopes have a .2nm resolving power

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16
Q

What is chromatic aberration?

A

The effect when many colors are seen toward the edge of the microscope field of vision (like a prisim)

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17
Q

How are microscopes corrected for chromatic aberration?

A

With a special achromatic lens (corrected for red and blue light) or by using a blue filter. The lab has blue filters in their microscopes which also increase resolution.

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18
Q

a warm cabinet that maintains optimal temperature for microbes to grow (37 degrees Celsius)

A

incubator

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19
Q

a steam sterilizer used to sterilize lab equipment at 121 Celsius or 249 Fahrenheit

A

autoclave

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20
Q

to transfer a culture of microorganisms from point A to point B by streaking the media (first step is to always sterilize the loop)

A

inocculation loop

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21
Q

transfer a culture of microorganisms by stabbing the media. (first step is to always sterilize the needle)

A

inocculation needle

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22
Q

weigh balance

A

measures mass

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23
Q

to stir liquids using a magnet that stirs the liquid.

A

stir plate

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24
Q

heats liquids to prepare for experiments

A

hot plate

25
Q

media

A

food for bacteria (agar, broth, deep, or slant)
1. nutrient agar
2. luria bertani
-selective= grows only one type of bacteria
-differential= can distinguish between organism types on one plate

26
Q

measure, hold and stores chemicals

A

glassware

27
Q

a closet that keeps everything steril

A

biosafety cabinet

28
Q

cool samples and slow bacterial growth

A

refrigerator

29
Q

shakes bacterial samples to allow growth

A

shaker

30
Q

heats media to optimal temp

A

water bath

31
Q

filters liquids and other chemical substances

A

filter paper

32
Q

helps to see different structures and to differentiate and ID organisms

A

stains

33
Q

chemically resistant gloves that protects hands (no latex allergy)

A

nitrile gloves

34
Q

transfer small volumes of liquid (sizes= p10, p20, p200, p1000)

A

micropipettes

35
Q

scrapes, move, or apply powders and to stir

A

spatula

36
Q

used for reactions and diagnostic testing

A

test tubes

37
Q

run DNA samples, proteins(RNA) and analyze by size and charge, also DNA fingerprinting, evolution, and paternity

A

gel electrophoresis

38
Q

gets a pellet into a solution

A

vortex

39
Q

take media, hardens it and grow microorganisms

A

petri dish

40
Q

separates solids and liquids by density

A

centrafuge

41
Q

microcentrafuge tubes

A

store small samples of samples/bacteria/liquid

42
Q

used to measure ph of a base or acid

A

ph meter

43
Q

used for preserving moisture-sensitive specimen

A

desiccator

44
Q

used to amplify segments of DNA via the polymerase chain reaction

A

thermocycler

45
Q

used for mixing

A

glass rods

46
Q

Used to hold the test tube in an up right position.

A

Test tube rack

47
Q

Used for filtration. (needs filter paper)

A

Funnel

48
Q

Used to measure accurately the volume of a liquid.

A

Volumetric glassware

49
Q

Permits fluid to flow in a drop wise fashion.

A

Dropping bottle

50
Q

Used for heating liquids and preparing reagent solutions. Example: beaker.

A

Non-volumetric glassware

51
Q

Produces a single flame at varying heights. Used for heating and sterilization.

A

Bunsen burner

52
Q

Serves as a repository for thermo labile chemicals, solutions, antibiotics, serums, and biochemical reagents at cooler temperatures. Used for storage of sterilized media, so as to prevent dehydration.

A

Refrigerator

53
Q

When enumerating bacteria, it’s assumed that a single bacterium gives rise to a single visible colony. The colony counter divides the plate into several square divisions, which makes counting much easier.

A

Quebec colony counter

54
Q

Some chemicals require stirring for a long time to be dissolved in certain solvents. Used to dissolve such substances easily and quickly.
A small teflon-coated magnet.

A

Magnetic stirrer

55
Q

Used for measuring the differences in color intensity/murkiness of solutions.

A

Spectrophotometer

56
Q

Used to seal petri dishes to prevent contamination (contamination of the bacteria in the dish and of us).

A

3M Parafilm

57
Q

Angled platform to allow agar to cool.

A

Test tube slant rack

58
Q

Used to protect the high-power objective lens when using oil immersion.

A

Finger cot

Microbiology, Mycology, Virology (8 decks)

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