Laboratory Equipment Flashcards

1
Q

focuses the light ging into the objective lens

A

Microscope- The Condenser

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

adjusts the amount of light going into the objective lens

A

Microscope- The Iris Diaphragm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

holds the objective lenses and rotates to change from one objective to another

A

Microscope- Nosepiece

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

moves the stage larger distances to help get the object in focus

A

Microscope- Coarse Objective Knob

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

moves the stage smaler increments to help get the object in better focus

A

Microscope- Fine Adjustment Knob

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

magnifies the object. It is located near the object.

A

Microscope- Objective Lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

magnifies the image of the object from the objective lens. It is located near the eye.

A

Microscope- Occular Lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Steps in using the microscope

A

1) scanner power in place
2) place slide of specimen stage in place with stage clips
3) Using the course adjustment knob, raise the stage all the way up and then down slowly until you can see image of specimen
4) Readjust with fine adjustment knob

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Describe the purpose of the oil immersion lens

A

Used when oil is added to the slide. Oil must touch the object and objective lens to that the light does not refract futher

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Parfocal

A

Parfocal microscopes are able to move from one objective to the next and still be in focus due to the length of the objective lenses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

The ability to distinguish between two objects that are very close together.

A

Resolution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is resolving power?

A

The measurement of resolution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are two ways to increase resolving power?

A
  1. By decreasing the wavelength of light (using a blue filter) 2. Increasing the numerical aperature
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What units are used to express resolving power?

A

nm or nanometers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is better, smaller or larger resolving power?

A

Smaller is better
Light microscopes have a 200nm resolving power
Electron microscopes have a .2nm resolving power

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is chromatic aberration?

A

The effect when many colors are seen toward the edge of the microscope field of vision (like a prisim)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

How are microscopes corrected for chromatic aberration?

A

With a special achromatic lens (corrected for red and blue light) or by using a blue filter. The lab has blue filters in their microscopes which also increase resolution.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

a warm cabinet that maintains optimal temperature for microbes to grow (37 degrees Celsius)

A

incubator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

a steam sterilizer used to sterilize lab equipment at 121 Celsius or 249 Fahrenheit

A

autoclave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

to transfer a culture of microorganisms from point A to point B by streaking the media (first step is to always sterilize the loop)

A

inocculation loop

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

transfer a culture of microorganisms by stabbing the media. (first step is to always sterilize the needle)

A

inocculation needle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

weigh balance

A

measures mass

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

to stir liquids using a magnet that stirs the liquid.

A

stir plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

heats liquids to prepare for experiments

25
media
food for bacteria (agar, broth, deep, or slant) 1. nutrient agar 2. luria bertani -selective= grows only one type of bacteria -differential= can distinguish between organism types on one plate
26
measure, hold and stores chemicals
glassware
27
a closet that keeps everything steril
biosafety cabinet
28
cool samples and slow bacterial growth
refrigerator
29
shakes bacterial samples to allow growth
shaker
30
heats media to optimal temp
water bath
31
filters liquids and other chemical substances
filter paper
32
helps to see different structures and to differentiate and ID organisms
stains
33
chemically resistant gloves that protects hands (no latex allergy)
nitrile gloves
34
transfer small volumes of liquid (sizes= p10, p20, p200, p1000)
micropipettes
35
scrapes, move, or apply powders and to stir
spatula
36
used for reactions and diagnostic testing
test tubes
37
run DNA samples, proteins(RNA) and analyze by size and charge, also DNA fingerprinting, evolution, and paternity
gel electrophoresis
38
gets a pellet into a solution
vortex
39
take media, hardens it and grow microorganisms
petri dish
40
separates solids and liquids by density
centrafuge
41
microcentrafuge tubes
store small samples of samples/bacteria/liquid
42
used to measure ph of a base or acid
ph meter
43
used for preserving moisture-sensitive specimen
desiccator
44
used to amplify segments of DNA via the polymerase chain reaction
thermocycler
45
used for mixing
glass rods
46
Used to hold the test tube in an up right position.
Test tube rack
47
Used for filtration. (needs filter paper)
Funnel
48
Used to measure accurately the volume of a liquid.
Volumetric glassware
49
Permits fluid to flow in a drop wise fashion.
Dropping bottle
50
Used for heating liquids and preparing reagent solutions. Example: beaker.
Non-volumetric glassware
51
Produces a single flame at varying heights. Used for heating and sterilization.
Bunsen burner
52
Serves as a repository for thermo labile chemicals, solutions, antibiotics, serums, and biochemical reagents at cooler temperatures. Used for storage of sterilized media, so as to prevent dehydration.
Refrigerator
53
When enumerating bacteria, it's assumed that a single bacterium gives rise to a single visible colony. The colony counter divides the plate into several square divisions, which makes counting much easier.
Quebec colony counter
54
Some chemicals require stirring for a long time to be dissolved in certain solvents. Used to dissolve such substances easily and quickly. A small teflon-coated magnet.
Magnetic stirrer
55
Used for measuring the differences in color intensity/murkiness of solutions.
Spectrophotometer
56
Used to seal petri dishes to prevent contamination (contamination of the bacteria in the dish and of us).
3M Parafilm
57
Angled platform to allow agar to cool.
Test tube slant rack
58
Used to protect the high-power objective lens when using oil immersion.
Finger cot