Twist NGS Product "cheat sheet"

Question 1

Twist Library Prep kits

Answer 1

takes DNA/RNA and gets it ready to be “read” by the sequencer. There are two types of ways to get the DNA/RNA library ready- Enzymatic frag or mechanical frag.

Question 2

Top selling points for library prep kits

Answer 2

1. Speed- you can go from raw genomic DNA to sequencer ready library in just 2 hours.
2. one stop shop- combines multiple steps (fixing ends, adding tails, sticking on adapters) into one “smooth move”. less mess. less stress.
3. Flexible- works with tiny DNA amounts (1 ng or bigger batches too). it’s like a recipe that works like you’re cooking just for one or a crowd.
4. High quality- gives you clean, uniform, consistent libraries for whole genome or targeted sequencing.

It’s the ultimate prepper- it gets your DNA dressed up and ready to shine without wasting time.

Question 3

Exome 2.0- The “gene hunter”

Answer 3

imagine your genome is an entire book, but you only care about the juicy chapters. (protein coding parts aka the exome). Exome 2.0 zooms in on those 1% of pages that hold 85% of the disease-causing secrets, ignoring the “noise”.

Question 4

Top selling points of Exome 2.0

Answer 4

1. super coverage- hits all the big databases (RefSeq, CCDS, Gencode, ClinVar*) plus sneaky non coding spots tied to diseases.
2. Efficiency- high uniformity and and low “oops” reads mean you get more bang for your buck and less wasted effort.
3. Disease detection work- built to spot rare inherited conditions and germline cancers.
4. customizable- add your own targets to tweak it for your needs. it’s not a one size fits all, it’s tailored to the research mission.

• Perfect for finding the “why” behind disease.

Question 5

FlexPrep (Ultra-High Throughput Kit)

Answer 5

it’s the overachiever for when you’ve got a TON of samples to sequence. (think thousands- like profiling every cow in a herd). it’s a library prep on steroids, designed for Agrigenomics (farm stuff) and population genomics (people stuff).

Question 6

Top selling points for FlexPrep:

Answer 6

1. massive scale- Handles up to 1,152 samples in one go on a single 96 well plate. (basically like cooking thanksgiving dinner for a whole town in one oven).
2. Cost efficient - cuts down on cost massively with “normalization by ligation” a fancy term for skipping annoying steps). and uses way less supplies. (12x less plastic waste than standard kits).
3. Automation ready- built for robots to take over, so humans can chill.
4. Multiplexing magic - tags samples early with barcodes, so you can mix tons of them without loosing track.

This is perfect for population genomic studies and can sequence anything from pigs to peanuts. (lol).

Question 7

RNA Seq- the RNA Rockstar of them all

Answer 7

RNA is the messenger that tells your cells what to do. (mRNA). this kit preps RNA (from fresh samples or crusty old FFPE ones) for sequencing. Either whole transcriptome (eveyrthing) or targeted (just the good bits like the RNA Exome).

Question 8

Top selling points for RNA - Seq

Answer 8

1. From RNA to library ready in 5 hours.
2. Low input, high output- works with as little as 1ng of RNA (tiny tiny) and still catches the quiet whispers of low-expression genes.
3. Exon-Aware RNA Exome- zooms into protein coding RNA without bias. catching rare isoforms and fusions (cancer clues) covers 99.9% of the “good stuff”.
4. Versatile- options for whole transcriptome or targeted panels (RNA exome or custom design). plus a depletion kit that gets rid of the pesky rRNA (ribosomal RNA) and globin noise.

This kit unlocks gene expression, fusions and variants in one slick package even from degraded samples (FFPE).