RNA Seq

Question 1

What are some customer pain points with RNA Seq

Answer 1

• My samples are fixed in FFPE blocks
• My technicians need to process so many samples

-Our current fusion solutions- i need more sensitivity to detect a different type of mutation.

• it would be great to cover both mutations and expression in a single assay. “i need better coverage”

Question 2

Explain the Twist standard 96-well RNA library kit

Answer 2

Designed for high-throughput RNA Seq. Ideal for processing large numbers of samples in a standardized format.

Question 3

Basics of RNA library prep kit at Twist

Answer 3

• High throughput (supports 96 samples in parallel, making it a great option for large scale RNA Seq studies)
  -The kit offers a streamlined, efficient workflow for converting RNA into Seq ready libraries, with minimal hands-on time.

-Input material- it can work with total RNA or selected RNA, making it versatile depending on the needs.

-consistent performance- the kit ensures uniform library prep.

• It is suitable for whole transcriptome seq, gene expression profiling and other RNA Seq based analysis.

Question 4

What is the Globin depletion kit?

Answer 4

This is important for removing unwanted sequences from the RNA sample before library prep to make it more “clean”. Globin depletion is our only option at Twist. We basically deplete the mRNA (drown out the additional noise) to allow for more targeted focus.

Question 5

Go into more detail about the globin depletion kit

Answer 5

This kit targets and depletes globin mRNA, which is useful when working with blood samples or other tissues where globin mRNA is abundant.

Question 6

What is really important about our RNA kit?

Answer 6

Works well with low input samples like FFPE.

Question 7

Key advantages of Twist RNA lib prep kit?

Answer 7

1. high throughput- both the standard 96 well kit and depletion options scale well for a large number of samples.
2. Flexibility- Adaptable to different types of RNA: total RNA/PolyA+ RNA) and compatible with a range of downstream sequencing platforms.
3. Quality- Twists reagents are engineered to ensure high-quality libraries with minimal bias, meaning- accurate representation of the transcriptome.

Question 8

Maximizing Twist RNA library prep on NovaSeq

Answer 8

Library quality is crucial before sample hits the sequencer**

input amount- usually 100ng of RNA is recommended.

• Since Twist offers the Ribosomal RNA Depletion and Globin depletion, we are able to reduce the pressence of the rRNA and globin sequences in your sample for efficient sequencing of the transcriptome.