Tumor Translocations

Question 1

Ewing/PNET? (4 of them)

Answer 1

t(11;22)EWSR1-FLI1 (90%)
t(21;22)EWSR1-ERG
t(17;22)EWSR1-E1AF

t(7;22)EWSR1-ATF1

Question 2

Clear cell sarcoma (malignant melanoma of soft parts)?

Desmoplastic Round Cell?

Answer 2

t(12;22)EWSR1-ATF1

t(11;22)EWSR1-WT1

Question 3

Myxoid Liposarcoma (2)?

Synovial Sarcoma?

Answer 3

t(12;16)FUS-CHOP (DDIT3) OR t(12;22) EWSR1-CHOP (DDIT3)

t(X;18)SYT-SSX1 or SYT-SSX2

Question 4

Alvelolar Rhabdomyosarcoma (2 translocations)?

Extraskeletal myxoid chondrosarcoma?

Answer 4

t(2;13) PAX3-FOXO1A OR t(1;13) PAX7-FOXO1A

t(9;22) EWSR1-NR4A3

Question 5

Inflammatory myofibroblastic tumor (2 of them)?

Dermatofibrosarcoma Protuberans?

Answer 5

t(1;2) TPM3-ALK1 or t(2;19) TMP4-ALK1

t(17;22) COL1A1-PDGFB

Question 6

What collecton tubes can be used for DNA?

What used to not be able to be used but can be used now?

Answer 6

EDTA (Lavender); ACD (Yellow)

Heparin (Green)

Question 7

How long to keep reports for for inherited disorders?

Who enforces CLIA?

Answer 7

25 years

CMS and CDC; CAP is deemed and FDA can enforce CLIA waived

Question 8

FDA cleared vs FDA approval?

Answer 8

Cleared: Similar to another device on the market; 510(k)
Approved; New test

Need to confirm precision, accuracy, reference range and reportable range