Tubeculosis

Question 1

At what temperature should skin specific mycobacterium be incubated at to identify?

Answer 1

30C (M. marinum, M. ulcerans, M. haemophilum)

Question 2

BacTec detects growth by what method?

Answer 2

Decrease in Oxygen leading to fluorescence.

Question 3

High contamination rates greater than 5% in TB should be reduced by using what?

Answer 3

Additional selective media

Question 4

How do you differentiate niacin positive Mycobacterium simiae from Myccobacterium tuberculosis complex?

Answer 4

M. simiae is positive for semi-qualitative catalase, pigment, and catalase at 68C. M. simiae is negative for nitrate, Accuprobe and NAAT.

Question 5

HPLC is used to identify mycobacteria based on what?

Answer 5

Extraction of cell wall mycolic acids

Question 6

What is the best way to prevent tuberculosis exposure?

Answer 6

Open safety cups under the bio-safety cabinet

Question 7

What is the flow rate of air in the BSC in TB?

Answer 7

70% recirculated air, inward face velocity of 75ft/min.

Question 8

What is the sensitivity of PCR when using a specimen from a smear-positive individual?

Answer 8

Greater than or equal to 95%

Question 9

What media do you use when doing the semi-qualitative catalase test?

Answer 9

Egg media (either LJ of ATS)

Question 10

Which enzyme does RFLP use?

Answer 10

Restriction endonuclease.

Question 11

Which group of mycobacterium are distinguished by salt tolerance?

Answer 11

Rapid growers

Question 12

Which media do you use for a combined Niacin-Nitrate test?

Answer 12

Egg media deeps(either LJ or ATS)

Question 13

Which mycobacterium are in the MTB complex?

Answer 13

Mycobacterium tuberculosis, M. bovis, M. africanum, M. microti, M. canetti