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Bacti Flashcards

1
Q

A gram negative diplococci that is oxidase positive is isolated from a clinical specimen. How should it be reported?

A

Gram negative diplococci that is oxidase positive isolated.

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2
Q

A question about anaerobes. Maybe what is the cause of most anaerobic infections?

A

Endogenous (coming from within the body, ie normal flora)

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3
Q

Can Treponemas be differentiated morphologically or serologically?

A

No, but location in certain geographical areas, clinical manifestation, dark-field and serological reactions can help.

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4
Q

Distinguishing feature of Brucella abortus?

A

Positive CO2, positive H2S

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5
Q

Distinguishing feature of Group B Strep (Strep agalactiae)?

A

Positive CAMP test and infant meningitis.

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6
Q

Distinguishing feature of Streptobacillus moniliformis?

A

Puffballs or string of pearls

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7
Q

Having a terminal spore is characteristic of which organism?

A

Clostridium tetani

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8
Q

How can you differentiate between Listeria and Francisella?

A

Listera: Gram positive rod, motile, oxidase negative, catalase positive. Francisella: Gram negative rod, non-motile, oxidase negative, catalase positive.

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9
Q

How can you differentiate between Neisseria and Actinobacter?

A

Actinobacter is oxidase negative and grows at 25C.

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10
Q

How do you differentiate between Salmonella and Edwardsiella?

A

Indole (Salmonella is indole negative)

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11
Q

How do you differentiate between the various species of Brucella?

A

H2S production, growth in CO2, urea, resistance/susceptibility to fuschin (dye solubility).

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12
Q

How do you differentiate Listeria monocytogens from Listeria innocua?

A

Beta hemolysis (L. monocytogens is positive). Also CAMP test.

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13
Q

How do you differentiate Vibrio cholera from other Vibrio species?

A

Vibrio cholera does not require the addition of NaCl to media to grow. Also, TCBS media (yellow colony).

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14
Q

How does O/F media allow for determination of oxidation?

A

Can be overlaid with mineral oil to make it an anaerobic environment.

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15
Q

How does O/F media work?

A

High carbs with low peptones

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16
Q

How does Salmonella typhi differ from non-typhi Salmonella?

A

Salmonella typhi has gas from glucose, is ornathine decarboxylase positive and citrate positive.

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17
Q

In the US, what is not a common cause of meningitis?

A

Staph aureus

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18
Q

Is Staphylococcus saprophyticus sensitive or resistant to Novobiocin?

A

Resistant

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19
Q

Is the toxin produced from Staphylococcus heat labile or stable?

A

Heat stable, pre-formed toxin (think Staph aureus, food poisoning, fried rice)

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20
Q

Neisseria gonorrhea ferments which carbs? What about neisseria meningitidis?

A

gonorrhea ferments glucose. N. meningitidis ferments glucose and maltose.

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21
Q

Streptococcus bovis belongs to which Lancefield group?

A

Group D: Non-enterococcus Streptococcus

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22
Q

Vibrio cholera 01 is which of the following: Classic/El Tor biotypes, Pandemic, Toxin producing or all of the above?

A

All of the above.

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23
Q

What are the best conditions to grow Camplyobacter in?

A

5% O2, 10% CO2, 85% N2 (Microaerophilic).

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24
Q

What are the biochemcial and clinical reasons for the ONPG test?

A

To identify late lactose fermenters. O-Nitrophenol turns yellow when positive.

25
What are the significant reactions for Streptococus bovis?
Optochin resistant, positive VP and Esculin, sensitive to SXT (Trimethoprim/sulfamethoxazole).
26
What are the significant reactions for Streptococus pneumoniae?
Optochin sensitive, positive bile solubility, alpha hemolytic, positive quelling reaction.
27
What are the significant reactions of Brucella?
Gram negative rod or coccobacillus, oxidase positive, catalase positive, urea positive, non-motile.
28
What carbohydrate is fermented by ALL members of Enterobacteriacea?
Glucose
29
What disease does Treponema carateum cause?
Pinta
30
What indicator is used for ensuring anaerobic conditions in media?
Methylene blue
31
What is the best specimen for Bordetella pertussis?
Nasopharyngeal swab (NP swab)
32
What is the beta-galactosidase relationship to ONPG?
Hydrolyzes ONPG to ONP and Galactose.
33
What is the biochemical reaction that produces indole?
Tryptophan reduced to Tryptophanase (Indole)
34
What is the definition of a disinfectant?
An agent that destroys pathogenic organisms, but not necessarily spore-forming organism.
35
What is the morphology of Franciscella tularensis?
Small, coccobacillus
36
What is the purpose of the lactose pre-enrichment broth for Salmonella in food?
Used to resuscitate damaged cells and allow for low number of organisms to increase.
37
What media is used to grow Bordetella?
Regan-Lowe, Bordet-Gengou, Jones-Kendrick charcoal
38
What medias can be used to grow Neisseria?
MTM, GC-Lect, NYC, Martin-Lewis, JEMBEC
39
What organism causes non-gonococcal urethritis?
Chlamydia trachomatis
40
What organism is a gram negative rod, TSI is K/K, oxidase positive, motility positive (polar flagella), glucose/lactose negative, growth at 42C?
Pseudomonas
41
What test can be used to differentiate Clostridium and Bacillus?
Catalase and aeobic endospore formation.
42
What tests of morphology can be used to differentiate between Group A Strep and Group B Strep?
Group B Strep is CAMP and Hippurate positive.
43
What tests of morphology can be used to differentiate between Listeria and Corynebacterium?
Motility and morphology
44
What tests of morphology can be used to differentiate between Staphylococcus epidermatidis and Staphylococcus aureus?
Staph aureus is coagulase positive
45
What tests of morphology can be used to differentiate between Stapylococcus and Streptococcus?
Staph is catalase positive
46
What tests of morphology can be used to differentiate between Streptococcus viridans and Streptococcus pneumoniae?
Strep pnsumo is Optichin sensitive and Bile solubility positive
47
Which bacteria can you get from a dog bite?
Pasturella multocida
48
Which bacteria causes rat bite fever?
Streptobacillus moniliformis
49
Which gram positive cocci can be lancet shaped?
Streptococcus pneumoniae
50
Which organism is a gram positive cocci, catalase negative, hippurate positive, CAMP positive?
Group B Strep (Streptococcus agalactiae)
51
Which organism is a gram positive cocci, catalase positive, Coagulase negative that causes endocarditis?
Staphylococcus epidermatidis
52
Which organism is a gram positive short rods, oxidase positive, lactose positive, H2S production, non-motile?
Erysipelothrix
53
Which organism requires cold enrichment?
Yersinia
54
Which organism requires X and V factors?
Haemophilus (influenzae, aegyptius, haemolyticus)
55
Which STD is asymptomatic and causes PID?
Chlamydia
56
Which test can be used to differentiate E. coli from Shigella?
ONPG
57
Which test can be used to differentiate Listeria and Enterococcus? Which test CANNOT?
Catalase can be used (listeria is positive, Enterococcus is negative). Bile esculin cannot be used (both are positive).
58
You isolate a gram negative diplococci identified as Neisseria gonorrhea. Under what circumstances should this be confirmed by additional testing?
Always, regardless of where or whom it's isolated from.
59
You isolate clear colonies from MAC agar that shows a 3+ reaction with Shigella Group A antiserum and no reaction to Group B, C, or D. What is the next step?
Perform biochemical tests to rule out isolate as E. coli

PHM State Test (7 decks)

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