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Lilac hospice > Trimming > Flashcards

Trimming Flashcards

1
Q

The process by which processed tissue is trimmed and cut into uniformly thin slices

A

MICROTOMY

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2
Q

Three parts of Microtome

A

Block holder (chuck)
Knife carrier and knife
Pawl,ratchet feed wheel and adjustment screws

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3
Q

For cutting serial sections of large blocks of paraffin embedded tissues

A

ROCKING MICROTOME

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4
Q

Invented by Minot in 1885-86
Used to cut paraffin embedded tissues

A

ROTARY MICROTOME

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5
Q

The knife and the block holder are brought together by upward and vertical motions
It is heavier and more stable than the rocking microtome

A

ROTARY MICROTOME

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6
Q

Invented by Adams in 1789

A

SLIDING MICROTOME

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7
Q

For cutting of celloidin-embedded tissues
Base sledge microtome

A

SLIDING MICROTOME

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8
Q

Invented by Queckett in 1848

A

FREEZING MICROTOME

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9
Q

A refrigerated apparatus used in fresh tissue microtomy

A

Cryostat

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10
Q

Used for cutting unembedded or gelatin-embedded blocks frozen in liquefied carbon dioxide, liquefied nitrogen,Freon and other refrigerants

A

FREEZING MICROTOME

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11
Q

Used for cutting tissue sections of 0.5 micra for electron microscopy

A

ULTRATHIN MICROTOME

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12
Q

Knives For electron microscopy

A

Glass knives

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13
Q

Knives For electron microscopy

A

Diamond knives

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14
Q

One side of the knife is flat while the other is concave

A

Plane-concave knife (25mm.in length)

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15
Q

Both sides are concave

A

Biconcave knife (120 mm. in length)

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16
Q

Both sides are straight

A

Plane-wedge knife (100mm in length)

17
Q

The angle formed between the cutting edges
27°-32°

A

Bevel angle

18
Q

The angle between the block face and lower facet of knife
10°-15°

A

Clearance angle

19
Q

Removal of gross nicks on the knife edge (coarse honing)
To remove blemishes

A

HONING

20
Q

Types of Honing

A

Belgian Yellow
Arkansas
Finecarborundum

21
Q

Gives the best result

A

Belgian Yellow

22
Q

10-20 strokes hone

A

plane-wedge knife

23
Q

20-40 strokes hone

A

biconcave knife

24
Q

Facilitates honing & removes metal cutting
Light oil
Dilute paraffin solution
Xylene

A

Lubricant

25
Q

Process whereby the “burr” formed during honing is removed and the cutting edge of the knife is polished

A

Stropping

26
Q

80-120 strokes on each side strope

A

Biconcave knife

Lilac hospice (4 decks)

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