Dehydrating agents

Question 1

especially used for tissue sections thatgmentary biopsies require urgent examinations such as fra
A very concentrated solution (above 80%) makes tissues
Hard,brittle and difficult to cut

Answer 1

ALCOHOL

Question 2

Routine; fast; cheap
Clear,colorless,flammable fluid

Answer 2

Ethanol

Question 3

Is a toxic dehydrating agent
For blood (does not lyze RBCs) and tissue films and for smear preparation

Answer 3

Methanol (methyl alcohol)

Question 4

Slow dehydrating agent
For plant and animal microtechniques
Less shrinkage and hardening effect
Recommended for tissues which do not require rapid processing

Answer 4

Butanol (butyl alcohol)

Question 5

Substitute for alcohol
Very rapid (urgent biopsies)

Answer 5

Acetone

Question 6

Dehydrates rapidly
Ethylene glycol ethers are combustible at 110-120°F and are toxic by inhalation, skin contact and ingestion

Answer 6

Cellosolve (ethylene glycol monoethyl ether)

Question 7

Fast
Does not harden tissue
Produces very little distortion
Minimal shrinkage
Soluble in alcohol, water ,ether ,benzene ,chloroform ,acetone, and xylene

Answer 7

Triethyl phosphate

Question 8

Acts both as a dehydrant and clearant
Produces less shrinkage than alcohol
Expensive
Toxic to the liver

Answer 8

Dioxane (diethylene dioxide)

Question 9

Has a very offensive odor
Vapor causes nausea,dizziness, anesthesia

Answer 9

Tetrahydrofuran

Question 10

The process of removing intercellular and extracellular water from the tissue
Done following fixation and prior to wax impregnation
For routine tissues and if infiltration is with non-water miscible medium.

Answer 10

DEHYDRATION