transposons

Question 1

what is the main difference between transposons and retrotransposons

Answer 1

transposons sometimes leave a copy of dna behind whereas retrotransposons always leave a copy behind

Question 2

how do retrotransposons create two copies of DNA

Answer 2

the dna that is the transposable elements gets copied to an rna intermediate element which gets reverse transcribed by transcriptase. 2nd portion gets integrated into new section of genome

Question 3

what is Ds

Answer 3

a point on chromosome 9 in maize that is easily breakable. It is a mutated and non-functional version of Ac

Question 4

what does Ac encode for

Answer 4

transposase

Question 5

why do diff transposases have different lengths of target side duplications (TSDs)?

Answer 5

due to diff lengths of staggered cuts

Question 6

process of translocation in prokaryotes:

Answer 6

1. transposase cuts an insertion site
2. an insertion sequence is inserted
3. host dna repair mechanisms fill gaps creating a flank host duplication

Question 7

what is a terminal inverted repeat

Answer 7

recognition site for transposase cleavage on both strands

Question 8

are transposase specific?

Answer 8

yes