Transpiration & Translocation Flashcards

1
Q

What is the xylem? What does it do

A

A tube of dead cells with their ends removed
Transports water and dissolved mineral ions from the root hair cells to the leaves in the shoot

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2
Q

Define transpiration stream

A

Flow/movement of water

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3
Q

Define transpiration

A

A loss of water - evaporation through the stomata

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4
Q

What is the transpiration stream

A

A high tension water column

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5
Q

How are xylem vessels adapted to cope with the transpiration stream

A

The xylem cells are dead so no cell contents are needed which creates a hollow tube
Cell walls break down at the ends of cells, this forms a continuous tube so the water column is unbroken
Cell walls are thick and reinforced with spiral and rings of lignin which provides strength

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6
Q

What does lignin do? Why is this good

A

Thickens vessels in a spiral arrangement rather than a continuous thickening allowing the xylem vessels to withstand tension
This is good because it means less lignin is needed so it is more efficient and also it is good because it can grow longer tubes more easily as the plant grows

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7
Q

What is the cohesion tension theory

A

The idea that water molecules form hydrogen bonds between one another and between the walls of the plant to stick together and be pulled up the transpiration stream

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8
Q

What is the process of cohesion tension

A

H2O molecules evaporate out of the stomata
The cohesivity which forms an electrostatic attraction between the positive hydrogen and negative oxygen of 2 water molecules (due to the polarity of the water) pulls a stream up and out of the xylem
This allows the movement of water through the xylem

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9
Q

Is transpiration active or passive? What does this mean

A

Passive so doesn’t require metabolic energy

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10
Q

What happens in the cohesion tension theory if a column is broken

A

Water can flow into adjacent xylem vessels via pits (gaps in the xylem tube)

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11
Q

Why wouldn’t the xylem be able to provide energy if transpiration was an active process

A

Because it is made of dead cells

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12
Q

What is the process of transpiration driven by

A

Driven by heat energy from the sun (evaporation of water)

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13
Q

Define capillary action

A

The ability of a liquid to flow in a narrow space without the assistance of, and in opposition to external forces, like gravity

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14
Q

What does capillary action propose

A

Proposes that the smaller the tube diameter, the further the liquid moves

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15
Q

What is the evidence for cohesion tension theory

A

Tree trunk diameter
The fact that water cannot move up the xylem vessel if it is broken
The fact that water does not leak out when the xylem vessel break

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16
Q

How is tree trunk diameter evidence for cohesion tension theory

A

During the day - High transpiration rate, therefore we can see there is more tension and therefore a negative pressure in the xylem. This causes a shrink in diameter
At night - Low transpiration rate, so there is less tension in the xylem, so the trunk increases in diameter
This can be observed using a data logger

17
Q

How does the fact that water cannot move up the xylem vessel if it is broken act as evidence for cohesion tension theory

A

If the xylem vessel is broken and air enters, the tree can no longer draw up water. So the continuous column is broken, because of this water molecules can no longer stick together
This is also why we shouldn’t get air bubbles in a potometer

18
Q

How does the fact that water does not leak out when the xylem vessel breaks act as evidence for cohesion tension theory

A

Air is drawn in. This is consistent with being under tension

19
Q

What factors affect the rate of transpiration

A

Water potential gradient
Light
Temperature
Air movement

20
Q

How does water potential gradient affect the rate of transpiration

A

The greater the H2O potential gradient between the inside and the outside of the leaf, the faster the water will move out and therefore the greater the rate of transpiration

21
Q

How does light affect the rate of transpiration

A

Photosynthesis occurs during the day, so the stomata will be open which means there will be water loss

22
Q

How does temperature affect the rate of transpiration

A

A rise in temperature increases the kinetic energy of water molecules. This increases evaporation and therefore transpiration
Also a rise in temperature increases the amount of water air can hold (humidity) which increases rate of transpiration

23
Q

How does air movement affect the rate of transpiration

A

Water diffuses through the stomata and accumulates as water vapor around the stomata. Wind then moves this vapor so there is less on the stomata which makes the concentration gradient higher. The higher conc grad means there is more transpiration

24
Q

What do potometers do? What are they used for

A

Measure how fast a plant cutting takes up water
They are used to work out the rate of transpiration of a plant
It can be used to investigate the uptake under different conditions

25
How does a potometer measure how fast a potometer takes up water
It does this by measuring the movement of the air bubble
26
What does the phloem do
Translocation Transports sap/sugars containing organic substances
27
What organic substances is in the sap that the phloem contains
Sucrose Amino acids Growth hormones (eg. gibberellins) mRNA
28
What is in the phloem (structure wise)
Sieve tubes Sieve plates Companion cells Plasmodesmata
29
What is the structure of sieve plates? How are cells arranged using them
Sieve plates have a small amount of cytoplasm at the edges of the cell They have no nucleus and few other organelles Cells are arranged end to end with sieve plates
30
What are sieve plates? What do they allow
Small holes in the end of the cell wall to allow the flow of sap along the sieve tube
31
What do companion cells do? What do they contain
They provide metabolic support for sieve tubes Contain all organelles
32
Where is plasmodesmata found? What does it do
Goes between companion and sieve cell Allows movement of molecules and substances through the phloem
33
What is the process of mass flow hypothesis in the phloem
Hydrogen is pumped out of the companion cell via active transport Hydrogen moves back into the companion cell, co transporting sucrose down the hydrogen concentration gradient (against the sucrose concentration gradient) Sucrose diffuses from the companion cells into the sieve cells, this lowers the water potential in the sieve cell Water moves into the sieve cell from neighboring xylem vessels The increase in the volume of water increases the hydrostatic pressure in the phloem This pressure gradient causes mass flow of phloem sap in one direction
34
Describe the process of translocation
Loading of sugar into the sieve tubes at the source reduces the water potential inside the sieve tube members. This causes the tube to take up water by osmosis This uptake of water causes a positive pressure that forces the sap to flow along the tube The pressure is relieved by the unloading of sugar and the consequent loss of water from the tube at the sink In the case of leaf to root translocation, the xylem recycles water from sink to source
35
Why do plants use sucrose and not glucose
Glucose is readily broken down by enzymes which are present in all cells as part of glycolysis, however sucrose is broken down by a less common enzyme so needs less effort to isolate and preserve for storage Sucrose is a non reducing sugar. With being less active it is easier to transport in solution, within the plants phloem
36
What is translocation
Movement of sucrose through the phloem
37
What is the mass flow hypothesis
How translocation works