Translation Flashcards
How many possible codons are there?
64
What is a triplet binding assay?
A test which proves synthetic trinucleotides can promote binding of specific tRNAs to ribosomes.
How many sense codons are there?
61
Where is the code not universal?
In mitochondria
What is tRNA?
The adaptor between codons and amino acids.
How does the tRNA link to its amino acid?
Through covalent bonding.
What part of the tRNA recognises the codon?
The anticodon
What defines the cloverleaf structure of tRNA?
4 stems and 4 loops
What do the hydrogen bonds between paired bases of the tRNA result in?
Its 3D structure
What is the open reading frame?
The protein coding sequence in a gene.
What is meant by the genetic code being degenerate?
Most amino acids have more that one codon.
What happens to the tRNA when it binds the amino acid?
It becomes charged
How does the tRNA associate with the mRNA molecule?
Through the anticodon loop
What is the function of aminoacyl-tRNA synthetase?
It catalyses the covalent linkage between the 3’ end of the tRNA and its cognate amino acid.
Which tRNAs does aminoacyl-tRNA synthetase recognise?
All isoaccepting tRNAs