DNA Structure and Replication Flashcards
What circumstantial evidence is there to support the presence of DNA?
Nuclei staining put DNA in the correct place.
What did Griffith prove?
That a chemical substance from one cell is capable of genetically transforming another cell.
What was the chemical substance that transformed the non virulent R strain to a virulent strain?
DNA
What was used to determine whether DNA or protein is the genetic material?
Bacteriophage T2
What is the function of Bacteriophage T2?
It enters E.coli cells and converts them into virus making machines.
How was it determined that DNA is the genetic material?
DNA of bacteriophage T2 enters the E.coli not proteins.
What did Rosalind Franklin discover using x-ray diffraction?
The structure of DNA.
What is Erwin Chargaff’s rule?
The number of A’s = number of T’s and the number of G’s = the number of C’s.
What did Crick and Watson determine?
The double helix.
How many nucleotides are there per turn?
10
Which groove has the more accessible bases?
The major groove.
How many hydrogen bonds are there between Guanine and Cytosine?
3
How many hydrogen bonds are there between Adenine and Thymine?
2
What is strand direction determined by?
Sugar phosphate bonds.
What is semiconservative replication?
Replication where each new DNA molecule has one old strand and one new strand.