DNA Structure and Replication Flashcards

1
Q

What circumstantial evidence is there to support the presence of DNA?

A

Nuclei staining put DNA in the correct place.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What did Griffith prove?

A

That a chemical substance from one cell is capable of genetically transforming another cell.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What was the chemical substance that transformed the non virulent R strain to a virulent strain?

A

DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What was used to determine whether DNA or protein is the genetic material?

A

Bacteriophage T2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the function of Bacteriophage T2?

A

It enters E.coli cells and converts them into virus making machines.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How was it determined that DNA is the genetic material?

A

DNA of bacteriophage T2 enters the E.coli not proteins.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What did Rosalind Franklin discover using x-ray diffraction?

A

The structure of DNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is Erwin Chargaff’s rule?

A

The number of A’s = number of T’s and the number of G’s = the number of C’s.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What did Crick and Watson determine?

A

The double helix.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How many nucleotides are there per turn?

A

10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Which groove has the more accessible bases?

A

The major groove.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

How many hydrogen bonds are there between Guanine and Cytosine?

A

3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How many hydrogen bonds are there between Adenine and Thymine?

A

2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is strand direction determined by?

A

Sugar phosphate bonds.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is semiconservative replication?

A

Replication where each new DNA molecule has one old strand and one new strand.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Who proved semiconservative replication?

A

Meselson-Stahl

17
Q

How does replication happen in bacteria?

A

It proceeds bidirectionally from a single origin on a circular genome.

18
Q

How do eukaryotic chromosomes differ from prokaryotic?

A

They are much longer with multiple origins.

19
Q

In what direction does DNA grow?

A

5’-3’

20
Q

What does DNA polymerase bind to?

A

The template strand.

21
Q

How is DNA polymerase shaped?

A

Like an open right hand - the “palm” brings active site and substrate into contact and the “fingers” recognise the nucleotide bases.

22
Q

What does DNA polymerase require to start synthesis?

A

A 3’OH end.

23
Q

How is an RNA primer produced?

A

By primase binding to the template strand and synthesising a primer.

24
Q

What enzyme unwinds the DNA?

A

Helicase

25
Q

What keeps the strands separated?

A

Single stranded binding proteins.

26
Q

How does synthesis on the lagging strand occur?

A

Discontinuous okazaki fragments are lay down and a primer is lay down to provide a 3’ end.

27
Q

What identifies mismatches?

A

DNA polymerase and then exonuclease removes the nucleotide and adds the correct one.

28
Q

In what phase of the cell cycle does DNA synthesis take place?

A

S phase.

29
Q

What is the process of mismatch repair?

A

Proofreading misses the error, mismatch repair proteins identify and remove the nucleotide and some of the surrounding nucleotides, DNA polymerase I adds the correct nucleotides and ligase repairs the remaining nick.

30
Q

What is the process of excision repair?

A

The nucleotide is damaged by external factors, excision repair proteins remove the damaged nucleotide and others surrounding it, DNA polymerase adds the correct nucleotides and ligase repairs the remaining nick.