Gene Regulation and Transcription Flashcards
What did Beadle and Tatum prove?
The one gene one polypeptide hypothesis
What is differential gene expression regulated by?
A combination of different transcription factors in cell differentiation.
What is the primary control point of gene expression?
Initiation of transcription genes
What causes only a fraction of genes in a cell to be expressed at one time?
Cells having distinct sets of transcription regulators and some regulators increasing transcription whereas some supress it.
How are prokaryote regulatory controlled?
By nutrient availability
What is the function of tRNA?
It is the adapter between amino acids and the mRNA sequence.
What is the function of mRNA?
It is the protein coding sequence of a gene.
What is the function of rRNA?
It is a structural and catalytic component of ribosomes.
What is the function of miRNA?
To inhibit translation.
What is the function of snRNA?
It is involved in RNA processing/splicing.
In what direction is RNA synthesised?
5’ to 3’
What is the function of RNA polymerase?
It melts DNA to expose ten base pairs at a time.
What is the transcription bubble?
It is where DNA is unwound in RNA polymerase.
Where does the phosphodiester bond form on mRNA?
Between the 5’ phosphate of the new group and the 3’ hydroxyl of the existing nucleic acid.
What are the components of transcription?
- nucleoside triphosphates (ATP, GTP, CTP and UTP)
- RNA polymerase
- DNA template
- pH buffer