Translation Flashcards
Where does the wobble hypothesis occur ?
3’ base of mRna
5’ base of TRNA anticodon
What does Amino Acyl TRNA synthetase do ?
Attaches activated AA to TRNA
Recognises both anticodon and attachment site of TRNA
What does shine dalgarno mRna sequence do ?
It is the 5’end of Prok Mrna
Pairs with 3’ end of 16s rRna
To what does tRna along with FMet interact with during initiation of translation ?
tRna along with FMET interacts with AUG, IF-2 and GTP
What happens once 50s ribosome attaches with Mrna bound with TRNA ?
IF-2 DISAASOCIATES
FULL 70s ribosome is ready for translation
What did EF-TU do in elongation ?
Cleaves AA from another TRNA and binds it to A-site
What is the function of EF-T in translation ?
Recyclers ADP TO ATP for EF-TU to cleave more amino acids
Which enzyme creates a peptide bond between AA in translation ?
Peptidyl transferace
What is the energy requirement for AA synthesis ?
4
What is the function of EF-G in translation ?
Hydrolyses GTP to GDP for translocation of ribosome
What are the 30s subunit inhibitors ?
Streptomycin - stops initiation
Tertracycleine - blocks A site
Puromycin - premature termination
What are the 50s subunit inhibitors ?
Chloramphenicol- inhibits peptidyl transferace and elongation
Erythromycin - inhibits translocation
What are the initiation factors of Eukaryotic translation ?
Elf-2 (binds tRna to Site)
EIF-4 (determines the placement of AUG START CODON)
Elongation factors of Eukaryotic translation ?
EEF-1- Guides AA tRna to A-site
EEF-2 - hydrolyses GTP for translocaton
What are the 2 toxins of Eukaryotic translation ?
Ricin - INACTIVATES peptidyl transferace
Diphtheria - INACTIVATES eEF-2
