Transcription - Lecture 1

Question 1

Does translation and transcription always occur simultaneously? If not - why?

Answer 1

In prokaryotes - yes

In eukaryotes - no bc transcription occurs in the nucleus and translation occurs in the cytoplasm

Question 2

What is the function of micro RNAs?

Answer 2

block expression of complementary mRNAs

Question 3

What is the function of long non-coding RNAs?

Answer 3

to regulate gene expression by binds to mRNA or micro-RNAs

Question 4

How is mRNA synthesized?

Answer 4

It is synthesized in the 5’ to 3’ direction but only using the 3’ to 5’ DNA template strand with help of RNA polymerase

Question 5

What is the function of gRNA?

Answer 5

direct the insertion of uridine bases into the mRNA by a repair polymerase through complementary pairing

Question 6

What does cytidine deaminase do?

Answer 6

Edits RNA by changing the “C” to “U”

Question 7

What effect does cytidine deaminase have on the ApoB?

Answer 7

In the ApoB gene the CAA codon can change to a stop UAA codon which truncates the protein and gives it a different function

Question 8

What is the function of the micro RNA siRNA?

Answer 8

binds to mRNA to inhibit translation

Question 9

What is the function of the micro RNA miRNA?

Answer 9

binds to mRNA and causes degradation