DNA Replication - Lecture 1 Flashcards
1
Q
How many base pairs located per turn of a DNA strand?
A
10
2
Q
How long is each turn of DNA?
A
3.4nm long
3
Q
What is conservative DNA replication?
A
A process of DNA replication that produces one fully original double helix and one fully synthesized double helix
4
Q
What is dispersive DNA replication?
A
A process of DNA replication where two double helices are produced but each contain fragments of original and synthesized DNA
5
Q
What is semiconservative DNA replication?
A
A process of DNA replication where two double helices are produced but each contained one strand of original DNA and one strand of fully synthesized DNA
6
Q
How was it determined that DNA replicated semi conservatively?
A
- E.coli was cultured in a medium containing heavy nitrogen so that on all the DNA also contains heavy nitrogen
- This heavy DNA was then centrifuged have one heavy density band was observed
- Then light nitrogen was added to the medium and one hybrid of medium density was observed
- Later after several rounds of the centrifuge, mostly light nitrogen bands were observed with some hybrid bands
7
Q
Who determined that DNA replicates semi-conservatively?
A
Meselon and Stahl
