Transcription Intro

Question 1

enzymes responsible for RNA synthesis are called ____ and dependent..

Answer 1

RNA polymerases and DNA dependent RNA polymerases

Question 2

RNAP reads from and synthesizes from…

Answer 2

reads template strand from 3’ to 5’ and synthesizes RNA in 5’ to 3’ direction

Question 3

what building unit does RNAP synthesize

Answer 3

RNA utilizing ribonucleoside triphosphate

Question 4

Transcription Diagram - Upstream

Answer 4

Towards 3’ direction of template strand

Question 5

Transcription Diagram - Downstream

Answer 5

toward 5’ direction of template strand

Question 6

Transcription Diagram - transcription region

Answer 6

nucleotides sequence transcribed by RNAP and located between terminator and promotor

Question 7

promotor

Answer 7

lies upstream and site where RNAP begins attachment

Question 8

terminator

Answer 8

lies downstream and at site where transcription is terminated

Question 9

start point

Answer 9

+1 , adjacent nucleotides are given positive number that increase as we go downstream transcription region (+1 , +2 , +3 )

Question 10

transcription unit

Answer 10

promotor, transcription region, terminator

Question 11

Steps of transcription

Answer 11

1. Template Binding & Formation of Pre initiation Complex
2. Initiation
3. Elongation
4. Termination

Question 12

Steps of transcription - Template binding and formation of pre initiation complex

Answer 12

binding of RNAP to promotor region

Question 13

Steps of transcription- initiation

Answer 13

RNAP moves on promotor til it reaches start point (+1)

Question 14

Steps of transcription- elongation

Answer 14

RNAP starts to synthesize complementary transcript using triphosphate (ATP, GTP, CTP, UTP) with release of pyrophosphates each time til termination signal is reached.

Question 15

Steps of transcription- termination

Answer 15

termination occurs when termination signal is reached.