Transcription

Question 1

Requires?

Answer 1

Control elements

Question 2

Control elements include?

Answer 2

1. Promoter
   - 5’TATAAAA3’
   - direct RNA polymerase to transcription start site
2. Proximal element
   - binding sites for GTP
3. Distal control elements
   - enhancers/ silences

Question 3

3 characteristics of distal control elements

Answer 3

1. Act over large distance
2. Independent on orientation
3. Independent of position

Question 4

Two types of transcription factors

Answer 4

GTF

STF

Question 5

Describe how GTF works

Answer 5

TFIID contains TATA binding protein and small TBP associated proteins;

Binds to TATA box;

Conformation change;

Attract other TF;

TIC;

RNA polymerase can bind

Question 6

Ha does enhancer works

Answer 6

Improved recruitment and interaction with promoter seq

Question 7

Describe three functions of silencers

Answer 7

1. Repressor binds to specific DNA site
   => block binding of protein req to initiate transcription
2. Repressor binds to DNA
   => interact with activator; preventing its fn
3. Interacts with GTF
   => prevent assemble of TIC