Transcription Flashcards

1
Q

Where does transcription take place in P and E

A

P - Cytoplasm
E - Nucleus

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2
Q

Name given to the stretch of DNA that is transcribed into RNA

A

Transcription unit

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3
Q

Name given to specific sequence that marks beginning of transcription

A

Promoter region

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4
Q

What are the 3 stages of transcription

A

Binding and initation
Elongation
Termination

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5
Q

Term used for the binding of RNA polymerase to the template strand

A

Template binding

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6
Q

Which strand is the antisense strand

A

Template strand

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7
Q

Where are the promoters found

A

Upstream from the point of initial transcription

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8
Q

What are used instead of primers during transcription and what is formed when RNA polymerase binds to the template strand

A

Transcription factors - Transcription initiation complex

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9
Q

What is the 4 base sequence used as a promoter in E

A

TATA

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10
Q

Why is transcription asymmetric

A

Only one strand serves as a template strand

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11
Q

Role of RNA polymerase in elongation

A

untwisting of double helix and assembling the RNA polynucleotide in the 5’ 3’ direction

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12
Q

How does termination differ in P and E

A

When the terminator sequence is transcribed in P the RNA polymerase detaches from the DNA and releases the mRNA immediately whereas in E detachment occurs 10-35 nucelotides downstream

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13
Q

What needs to occur before translation and exiting of mRNA from the nucelus

A

Post-transcriptional processing

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14
Q

Which end does capping (G) and tailing (A) occur and what are the 3 reasons for it

A

Capping - 5’
Tailing - 3’
Prevents degredation against hydrolytic enzymes
Facilitates exit of mRNA from nucleus
Facilitates ribosomal attachment

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15
Q

What does splicing remove and why does it occur

A

Introns - they are non-coding stretches of nucleotides

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16
Q

What molecule carries out splicing

A

Spliceosome