TRANSAMINATION AND OXIDATIVE DEAMINATION

Question 1

Two stages of amino acid degradation

Answer 1

1) Removal of the a-amino group
2) Degradation of the remaining carbon skeleton

Question 2

two types of Removal of the a-amino group

Answer 2

a. Transamination
b. Oxidative deamination

Question 3

two amino acids produced in transamination reactions

Answer 3

glutamate (from a-ketoglutarate)
aspartate (from
oxaloacetate)

Question 3

needed enzyme for a transamination reaction

Answer 3

aminotransferase

Question 4

always involve two amino acids (one as a reactant and one as a product) and two keto acids (one as a reactant and one as a product)

Answer 4

Transamination Reactions

Question 5

TRUE / FALSE:
There is no loss or gain of amino groups in transamination

Answer 5

TRUE

Question 6

Two keto/amino acid pairs are involved, with the members of a pair having a common carbon-chain base

Answer 6

Transamination Reactions

Question 7

transamination reaction involves several steps and requires the presence of

Answer 7

pyridoxal phosphate
(vitamin B6

Question 8

two most encountered keto/amino acid pairs in transamination

Answer 8

a -ketoglutarate / glutamate
oxaloacetate / aspartate

Question 9

reactions are reversible and can easily go in either direction, depending on the reactant concentrations

Answer 9

Transamination reactions

Question 10

a biochemical reaction that involves the
interchange of the amino group of an a-amino acid with the keto group of an
a-keto acid

Answer 10

transamination reaction

Question 11

occurs primarily in liver and kidney mitochondria

Answer 11

Oxidative deamination

Question 12

This enzyme is unusual in that it is the only known enzyme that can function with either NADP+ or NAD+ as a coenzyme

Answer 12

glutamate dehydrogenase

Question 12

the basis for regulation of amino acid concentrations
in the body

Answer 12

reversibility

Question 13

This coenzyme is an integral part of the transamination process

Answer 13

pyridoxal phosphate

Question 14

two pathways for further processing of the many glutamate molecules produced via transamination

Answer 14

• second transamination reaction that produces the amino acid aspartate
• production of
  ammonium ion (NH4 +) via oxidative deamination

Question 14

the “amino group carriers,”

Answer 14

ammonium ion the aspartate

Question 14

product of oxidative deamination

Answer 14

a-ketoglutarate

Question 15

a biochemical reaction in which an a-amino acid is converted into an aketo acid with release of an ammonium ion

Answer 15

oxidative deamination
reaction