Trans- proteomics

Question 1

What is a proteome

Answer 1

A set of proteins in an organism etc

Question 2

Targeted and non targeted method to study proteins

Answer 2

Targeted: antibodies ( myriad, multiplex)

Aptamers- DNA based
O link protomics

Non targeted:

Mass spectrometry

Question 3

How to break proteins into peptides

Answer 3

Break disulphide bonds
C. Cysteine residues to prevent reforming
Use a protease ( trypsin)

Question 4

How to measure alot of peptides in a digested proteome?

Answer 4

Overtime by reverse phase chromatography

Question 5

Discovery proteomics

Answer 5

Global protein profiling

Semi quantitatively measures protein in a specific sample for differential expression

Question 6

Profiling methods pros and cons

Answer 6

Label free labelling - iTRAQ ,SILAC

Pros cheaper more sensitive
Cons
More machine time required and increased run variability

Question 7

Use of quality control in proteomics

Answer 7

Allows assessment of accuracy , precision, reproducibility

Run 2-3 times every 5-10 samples

Question 8

How is a single cell isolated

Answer 8

Using known cell surface markers by flow cytometry

Question 9

What is spacial proteomics

Answer 9

Study of sub cellulsr localisation by high throughput imaginf or quantitative MS

Question 10

What is targeted proteomics

Answer 10

Quantitation of a specific protein in a complex sample using MRM,STM or triple quadruple mass

For clinical use

Question 11

How proteomics applied to translational medicine

Answer 11

Biomarkers:

Diagnose disease
Treatment monitoring
Strategy disease serenity