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Basic genetics > Proteomics > Flashcards

Proteomics Flashcards

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Biology 101 flashcards
1
Q

What is proteomics

A

The study of a proteome
The main aim is quantification

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2
Q

How many essential amino acids

A

20

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3
Q

Why use proteomic technologies

A

To identify and wuantify overall proteins better than genomics

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4
Q

What are the challenges with studying proteomics (6)

A

Proteins are complex and dynamic
Low abundance
Quantification
Protein protein interaction
Data analysis
Post translational modifications

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5
Q

How to overcome challenges

A

By using fractionation and seperation methods to seperate into simpler fractions
Immunodepltion to remove abundant proteins

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6
Q

What is mass spec

A

Measuring accurate molecular mass

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7
Q

What is CID

A

Collision induced dissociation
Fragmentation technique for ions most selected

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8
Q

What quantitative technologies are there (3)

A

Label free- measures peptide ion intensity
Chemical mass tagging (TMT)
In vitro metabolic labelling SILA

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9
Q

What os metabolomics

A

The study of chemical processes involving metabolites
Provides a snapchot of cellular phenotype

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10
Q

What applications are used in cancer research (5)

A

Cancer biomarker discovery
Targegted Ms based assay
Functional characterisation
P-P interactions
Identification of PTM

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11
Q

What is ELISA

A

Enzyme linked immunosorbent assay
A capture antibody, second antibody + 3rd enzyme linked HRP antibody
Detects antibodies on the blood

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12
Q

What is the Proteome Human atlas

A

Large scale IHC

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13
Q

3 parts of a mass analyser

A

Ion source
Mass analyser
Detector

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14
Q

Types of mass analyser

A

Time of flight, quadruple, ion trap, orbitrap

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15
Q

Ionisation methods

A

MALDI

Electrospray ionisation

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16
Q

Ideal sample for MS

A

Can change to gaseous state, ionisable, stable and can be digested by a protease ( trypsin)

17
Q

Protein seperation methods

A

Electrophoresis:
GEL electrophoresis- size and charge
Free flow capillary electrophoresis- based on charge only

liquid chromatography:
Size exclusion- molecular size / retention time

Ion exchange- charge

Reverse phase LC- hydrophobic interaction

Affinity- antigen- antigen ( binding properties)

18
Q

What technique is metabolomics validated by?

A

ELISA
protein arrays and large scale IHC

19
Q

Methods to improve proteomic coverage

A

Fractionation and enrichment methods

Basic genetics (9 decks)

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